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Literature summary for 3.5.5.5 extracted from

  • Kiziak, C.; Conradt, D.; Stolz, A.; Mattes, R.; Klein, J.
    Nitrilase from Pseudomonas fluorescens EBC191: cloning and heterologous expression of the gene and biochemical characterization of the recombinant enzyme (2005), Microbiology, 151, 3639-3648.
    View publication on PubMed
Search for more literature for 3.5.5.5

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas fluorescens

General Stability

General Stability Organism
significant stabilization by addition of 1 M ammonium sulfate Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37700
-
 x * 37700, calculated Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-
Pseudomonas fluorescens EBC191
-
-
-

Storage Stability

Storage Stability Organism
-20°C, pH 7.5, 14 days, 90% loss of activity, addition of 1 M ammonium sulfate almost completely stabilizes Pseudomonas fluorescens
8°C, pH 7.5, 14 days, 75% loss of activity, addition of 1 M ammonium sulfate almost completely stabilizes Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 2-phenylglycinonitrile + 3 H2O 80% of the activity with mandelonitrile. 35% enantiomeric excess for S-configuration in acid formation, 10.26% of product is amide with 92% enantiomeric excess for S-configuration Pseudomonas fluorescens 2-phenylglycine + 2-amino-2-phenylacetamide + NH3
-
 ?
2-phenylpropionitrile + 2 H2O
-
 Pseudomonas fluorescens 2-phenylpropionic acid + NH3 12.5% of the activity with mandelonitrile. 65% enantiomeric excess for S-configuration in acid formation, 0.25% of product is amide with 6% enantiomeric excess for R-configuration ?
2-phenylvaleronitrile + H2O 700% of the activity with mandelonitrile Pseudomonas fluorescens 2-phenylvaleric acid + NH3
-
 ?
2-thiopheneacetonitrile + H2O 625% of the activity with mandelonitrile, 1.5% of product is amide Pseudomonas fluorescens 2-thiopheneacetic acid + NH3
-
 ?
2-thiopheneacetonitrile + H2O 625% of the activity with mandelonitrile, 1.5% of product is amide Pseudomonas fluorescens EBC191 2-thiopheneacetic acid + NH3
-
 ?
3-thiopheneacetonitrile + H2O 107% of the activity with mandelonitrile, 1.5% of product is amide Pseudomonas fluorescens 3-thiopheneacetic acid + NH3
-
 ?
3-thiopheneacetonitrile + H2O 107% of the activity with mandelonitrile, 1.5% of product is amide Pseudomonas fluorescens EBC191 3-thiopheneacetic acid + NH3
-
 ?
indole-3-acetonitrile + H2O 22% of the activity with mandelonitrile, 0.1% of product is amide Pseudomonas fluorescens indole-3-acetic acid + NH3
-
 ?
mandelonitrile + 2 H2O 31% enantiomeric excess for R-configuration in acid formation, 19% of product is amide with 85% enantiomeric excess for S-configuration Pseudomonas fluorescens mandelic acid + NH3
-
 ?
mandelonitrile + 2 H2O 31% enantiomeric excess for R-configuration in acid formation, 19% of product is amide with 85% enantiomeric excess for S-configuration Pseudomonas fluorescens EBC191 mandelic acid + NH3
-
 ?
additional information enzyme acts as nitrilase and hydratase. relative amounts of amides formed from different nitriles increase with an increasing negative inductive effect of the substituent in 2-position. Acids and amides formed from chiral nitriles demonstrate mostly opposite enantiomeric excess Pseudomonas fluorescens ?
-
 ?
additional information enzyme acts as nitrilase and hydratase. relative amounts of amides formed from different nitriles increase with an increasing negative inductive effect of the substituent in 2-position. Acids and amides formed from chiral nitriles demonstrate mostly opposite enantiomeric excess Pseudomonas fluorescens EBC191 ?
-
 ?
phenylacetonitrile + 2 H2O 206% of the activity with mandelonitrile, 3.9% of product is amide Pseudomonas fluorescens phenylacetic acid + NH3
-
 ?
phenylacetonitrile + 2 H2O 206% of the activity with mandelonitrile, 3.9% of product is amide Pseudomonas fluorescens EBC191 phenylacetic acid + NH3
-
 ?
valeronitrile + H2O 12.5% of the activity with mandelonitrile Pseudomonas fluorescens valeric acid + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 37700, calculated Pseudomonas fluorescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas fluorescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 complete loss of activity Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Pseudomonas fluorescens