BRENDA - Enzyme Database show
show all sequences of 3.5.5.5

Purification and characterization of an enantioselective arylacetonitrilase from Pseudomonas putida

Banerjee, A.; Kaul, P.; Banerjee, U.C.; Arch. Microbiol. 184, 407-418 (2006)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
-
Pseudomonas putida
Co2+
-
Pseudomonas putida
Cu2+
-
Pseudomonas putida
Fe2+
-
Pseudomonas putida
Hg2+
-
Pseudomonas putida
Ni2+
-
Pseudomonas putida
Zn2+
-
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.61
-
phenyl acetonitrile
35°C, pH 7.5
Pseudomonas putida
5.31
-
indole 3-acetonitrile
35°C, pH 7.5
Pseudomonas putida
13.4
-
mandelonitrile
35°C, pH 7.5
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
9 or 10 * 43000, SDS-PAGE
Pseudomonas putida
412000
-
gel filtration
Pseudomonas putida
Organic Solvent Stability
Organic Solvent
Commentary
Organism
dimethyl sulfoxide
2.5 or 5%, up to 20% increase in activity, decrease above
Pseudomonas putida
Ethanol
5%, 63% enhancement in activity probably due to increased availibaility of substrate. Above 10% ethanol, significant decrease in activity
Pseudomonas putida
Methanol
5%, 78% enhancement in activity probably due to increased availibaility of substrate. Above 10% methanol, significant decrease in activity
Pseudomonas putida
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas putida
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.26
-
35°C, pH 7.5
Pseudomonas putida
Storage Stability
Storage Stability
Organism
-80°C, 0.01 M phosphate, pH 7.5, 1 mM dithiothreitol, 20% glycerol, stable
Pseudomonas putida
4°C, 0.01 M phosphate, pH 7.5, 1 mM dithiothreitol, rapid loss of activity, presence of 20% glycerol stabilizes
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-chlorophenyl acetonitrile + H2O
76.1% of the activity with mandelonitrile
667345
Pseudomonas putida
2-chlorobenzoic acid + NH3
-
-
-
ir
2-methoxyphenyl acetonitrile + H2O
44.5% of the activity with mandelonitrile
667345
Pseudomonas putida
2-methoxybenzoic acid + NH3
-
-
-
ir
2-thiophene acetonitrile + H2O
137% of the activity with mandelonitrile
667345
Pseudomonas putida
thiophene-2-carboxylic acid + NH3
-
-
-
ir
4-aminophenyl acetonitrile + H2O
502.3% of the activity with mandelonitrile
667345
Pseudomonas putida
4-aminobenzoic acid + NH3
-
-
-
ir
4-chlorophenyl acetonitrile + H2O
360.3% of the activity with mandelonitrile
667345
Pseudomonas putida
4-chlorobenzoic acid + NH3
-
-
-
ir
4-hydroxy benzonitrile + H2O
23.1% of the activity with mandelonitrile
667345
Pseudomonas putida
4-hydroxybenzoate + NH3
-
-
-
ir
4-hydroxyphenyl acetonitrile + H2O
435% of the activity with mandelonitrile
667345
Pseudomonas putida
4-hydroxybenzoic acid + NH3
-
-
-
ir
adiponitrile + H2O
17.2% of the activity with mandelonitrile
667345
Pseudomonas putida
adipic acid + NH3
-
-
-
ir
cyclohexane carbonitrile + H2O
12.5% of the activity with mandelonitrile
667345
Pseudomonas putida
cyclohexlycarboxylic acid + NH3
-
-
-
ir
hex-5-enenitrile + H2O
7.9% of the activity with mandelonitrile
667345
Pseudomonas putida
hex-5-enoic acid + NH3
-
-
-
ir
indole 3-acetonitrile + H2O
319.9% of the activity with mandelonitrile
667345
Pseudomonas putida
(indol-3-yl)acetic acid + NH3
-
-
-
ir
isovaleronitrile + H2O
11.4% of the activity with mandelonitrile
667345
Pseudomonas putida
isovaleric acid + NH3
-
-
-
ir
mandelonitrile + 2 H2O
-
667345
Pseudomonas putida
(R)-mandelic acid + NH3
99.9% enantiomeric excess for (R)-isomer
-
-
ir
phenoxy acetonitrile + H2O
232.6% of the activity with mandelonitrile
667345
Pseudomonas putida
phenoxyacetate + NH3
-
-
-
ir
phenyl acetonitrile + H2O
342.3% of the activity with mandelonitrile
667345
Pseudomonas putida
benzoic acid + NH3
-
-
-
ir
phenyl glycinenitrile + H2O
192.5% of the activity with mandelonitrile
667345
Pseudomonas putida
phenylalanine + NH3
-
-
-
ir
phenyl thioacetonitrile + H2O
187.7% of the activity with mandelonitrile
667345
Pseudomonas putida
thiophenylacetate + NH3
-
-
-
ir
phenylacetonitrile + H2O
-
667345
Pseudomonas putida
phenylacetate + NH3
-
-
-
ir
Subunits
Subunits
Commentary
Organism
decamer
9 or 10 * 43000, SDS-PAGE
Pseudomonas putida
nonamer
9 or 10 * 43000, SDS-PAGE
Pseudomonas putida
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Pseudomonas putida
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
-
21% of maximum activity
Pseudomonas putida
70
-
11% of maximum activity
Pseudomonas putida
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
6 h, 87% residual activity
Pseudomonas putida
40
-
half-life 76 min
Pseudomonas putida
50
-
half-life 9 min
Pseudomonas putida
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1
-
phenyl acetonitrile
35°C, pH 7.5
Pseudomonas putida
11.8
-
mandelonitrile
35°C, pH 7.5
Pseudomonas putida
13.4
-
indole 3-acetonitrile
35°C, pH 7.5
Pseudomonas putida
18.85
-
phenyl acetonitrile
35°C, pH 7.5
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Pseudomonas putida
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
-
half-life 36 min
Pseudomonas putida
7
-
half-life 110 min
Pseudomonas putida
7.5
-
half-life 87 min
Pseudomonas putida
8
-
half-life 53 min
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
-
Pseudomonas putida
Co2+
-
Pseudomonas putida
Cu2+
-
Pseudomonas putida
Fe2+
-
Pseudomonas putida
Hg2+
-
Pseudomonas putida
Ni2+
-
Pseudomonas putida
Zn2+
-
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.61
-
phenyl acetonitrile
35°C, pH 7.5
Pseudomonas putida
5.31
-
indole 3-acetonitrile
35°C, pH 7.5
Pseudomonas putida
13.4
-
mandelonitrile
35°C, pH 7.5
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
9 or 10 * 43000, SDS-PAGE
Pseudomonas putida
412000
-
gel filtration
Pseudomonas putida
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
dimethyl sulfoxide
2.5 or 5%, up to 20% increase in activity, decrease above
Pseudomonas putida
Ethanol
5%, 63% enhancement in activity probably due to increased availibaility of substrate. Above 10% ethanol, significant decrease in activity
Pseudomonas putida
Methanol
5%, 78% enhancement in activity probably due to increased availibaility of substrate. Above 10% methanol, significant decrease in activity
Pseudomonas putida
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.26
-
35°C, pH 7.5
Pseudomonas putida
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, 0.01 M phosphate, pH 7.5, 1 mM dithiothreitol, 20% glycerol, stable
Pseudomonas putida
4°C, 0.01 M phosphate, pH 7.5, 1 mM dithiothreitol, rapid loss of activity, presence of 20% glycerol stabilizes
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-chlorophenyl acetonitrile + H2O
76.1% of the activity with mandelonitrile
667345
Pseudomonas putida
2-chlorobenzoic acid + NH3
-
-
-
ir
2-methoxyphenyl acetonitrile + H2O
44.5% of the activity with mandelonitrile
667345
Pseudomonas putida
2-methoxybenzoic acid + NH3
-
-
-
ir
2-thiophene acetonitrile + H2O
137% of the activity with mandelonitrile
667345
Pseudomonas putida
thiophene-2-carboxylic acid + NH3
-
-
-
ir
4-aminophenyl acetonitrile + H2O
502.3% of the activity with mandelonitrile
667345
Pseudomonas putida
4-aminobenzoic acid + NH3
-
-
-
ir
4-chlorophenyl acetonitrile + H2O
360.3% of the activity with mandelonitrile
667345
Pseudomonas putida
4-chlorobenzoic acid + NH3
-
-
-
ir
4-hydroxy benzonitrile + H2O
23.1% of the activity with mandelonitrile
667345
Pseudomonas putida
4-hydroxybenzoate + NH3
-
-
-
ir
4-hydroxyphenyl acetonitrile + H2O
435% of the activity with mandelonitrile
667345
Pseudomonas putida
4-hydroxybenzoic acid + NH3
-
-
-
ir
adiponitrile + H2O
17.2% of the activity with mandelonitrile
667345
Pseudomonas putida
adipic acid + NH3
-
-
-
ir
cyclohexane carbonitrile + H2O
12.5% of the activity with mandelonitrile
667345
Pseudomonas putida
cyclohexlycarboxylic acid + NH3
-
-
-
ir
hex-5-enenitrile + H2O
7.9% of the activity with mandelonitrile
667345
Pseudomonas putida
hex-5-enoic acid + NH3
-
-
-
ir
indole 3-acetonitrile + H2O
319.9% of the activity with mandelonitrile
667345
Pseudomonas putida
(indol-3-yl)acetic acid + NH3
-
-
-
ir
isovaleronitrile + H2O
11.4% of the activity with mandelonitrile
667345
Pseudomonas putida
isovaleric acid + NH3
-
-
-
ir
mandelonitrile + 2 H2O
-
667345
Pseudomonas putida
(R)-mandelic acid + NH3
99.9% enantiomeric excess for (R)-isomer
-
-
ir
phenoxy acetonitrile + H2O
232.6% of the activity with mandelonitrile
667345
Pseudomonas putida
phenoxyacetate + NH3
-
-
-
ir
phenyl acetonitrile + H2O
342.3% of the activity with mandelonitrile
667345
Pseudomonas putida
benzoic acid + NH3
-
-
-
ir
phenyl glycinenitrile + H2O
192.5% of the activity with mandelonitrile
667345
Pseudomonas putida
phenylalanine + NH3
-
-
-
ir
phenyl thioacetonitrile + H2O
187.7% of the activity with mandelonitrile
667345
Pseudomonas putida
thiophenylacetate + NH3
-
-
-
ir
phenylacetonitrile + H2O
-
667345
Pseudomonas putida
phenylacetate + NH3
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
decamer
9 or 10 * 43000, SDS-PAGE
Pseudomonas putida
nonamer
9 or 10 * 43000, SDS-PAGE
Pseudomonas putida
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Pseudomonas putida
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
-
21% of maximum activity
Pseudomonas putida
70
-
11% of maximum activity
Pseudomonas putida
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
6 h, 87% residual activity
Pseudomonas putida
40
-
half-life 76 min
Pseudomonas putida
50
-
half-life 9 min
Pseudomonas putida
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1
-
phenyl acetonitrile
35°C, pH 7.5
Pseudomonas putida
11.8
-
mandelonitrile
35°C, pH 7.5
Pseudomonas putida
13.4
-
indole 3-acetonitrile
35°C, pH 7.5
Pseudomonas putida
18.85
-
phenyl acetonitrile
35°C, pH 7.5
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Pseudomonas putida
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
-
half-life 36 min
Pseudomonas putida
7
-
half-life 110 min
Pseudomonas putida
7.5
-
half-life 87 min
Pseudomonas putida
8
-
half-life 53 min
Pseudomonas putida
Other publictions for EC 3.5.5.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733193
Sosedov
Improvement of the amides form ...
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-
-
1
-
1
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733558
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37
1655-1661
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-
-
1
-
-
-
-
1
-
-
1
-
-
4
-
-
1
-
-
-
-
-
41
1
1
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
-
41
1
1
1
1
-
1
-
-
-
-
-
-
-
-
-
733546
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61
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-
-
-
-
-
1
10
1
-
1
2
-
-
3
-
-
1
-
-
-
-
-
8
1
1
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
10
-
1
-
1
2
-
-
-
-
1
-
-
-
-
8
1
1
-
1
1
1
-
-
-
-
-
-
-
-
-
718648
Baum
Conversion of sterically deman ...
Pseudomonas fluorescens, Pseudomonas fluorescens EBC191
Appl. Environ. Microbiol.
78
48-57
2012
-
1
1
-
11
-
-
1
-
-
-
2
-
10
-
-
-
-
-
-
-
-
14
-
1
-
-
-
2
-
-
-
-
-
-
-
1
1
-
-
11
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
14
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
718670
Petrickova
Purification and characterizat ...
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Appl. Microbiol. Biotechnol.
93
1553-1561
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-
-
2
-
-
-
-
6
-
-
-
6
-
7
-
-
2
-
-
-
-
-
24
-
2
-
8
-
2
-
9
-
-
-
-
-
-
3
-
-
-
-
-
-
-
9
-
-
-
6
-
-
-
3
-
-
-
-
24
-
3
-
12
-
3
-
14
-
-
1
1
-
-
-
734496
Petrickova
-
Influence of point mutations n ...
Aspergillus niger, Aspergillus niger CBS 513.88, Neurospora crassa, Neurospora crassa DSM 1257
J. Mol. Catal. B
77
74-80
2012
-
-
2
-
4
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
8
-
-
-
-
-
2
4
-
-
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
2
4
-
-
-
-
-
-
-
-
719297
Detzel
-
Autodisplay of nitrilase from ...
Alcaligenes faecalis, Alcaligenes faecalis ATCC 8750
ChemCatChem
3
719-725
2011
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
719629
Liu
Gene cloning, expression, and ...
Alcaligenes faecalis, Alcaligenes faecalis ZJUTB10
J. Agric. Food Chem.
59
11560-11570
2011
-
1
1
-
3
-
11
1
-
2
1
2
-
2
-
-
1
1
-
-
-
-
12
2
1
1
1
-
1
-
-
-
-
-
-
-
1
1
-
-
3
-
-
11
-
1
-
2
1
2
-
-
-
1
-
-
-
-
12
2
1
1
1
-
1
-
-
-
-
1
1
-
-
-
710940
Sosedov
Construction and application o ...
Pseudomonas fluorescens
Appl. Environ. Microbiol.
76
3668-3674
2010
-
-
-
-
14
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712643
He
Biocatalytic synthesis of (R)- ...
Alcaligenes faecalis
J. Ind. Microbiol. Biotechnol.
37
741-750
2010
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
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5
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1
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1
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1
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8
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2
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6
1
1
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1
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2
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6
1
1
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1
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713073
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Pseudomonas syringae pv. syrin ...
no activity in no activity in Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. syringae, Pseudomonas syringae pv. syringae B728a
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4
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1
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2
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1
1
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1
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2
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1
1
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1
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2
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1
1
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1
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26
1
1
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1
1
1
1
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1
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1
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3
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1
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26
1
1
-
1
1
1
1
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694107
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10
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2
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1
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1
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-
1
1
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1
2
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1
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10
-
2
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1
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1
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12
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1
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12
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Kiziak
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1
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1
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4
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1
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1
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1
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4
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1
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1
2
18
2
1
2
3
4
1
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4
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7
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3
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2
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3
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1
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1
2
18
2
1
2
3
4
1
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4
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Mateo
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1
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1
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1
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2
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1
1
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Mateo
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Synthesis of enantiomerically ...
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Kiziak
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1
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1
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1
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2
15
1
1
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13
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1
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1
4
33
1
2
1
3
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1
1
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