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Literature summary for 3.5.5.5 extracted from

  • Banerjee, A.; Kaul, P.; Banerjee, U.C.
    Purification and characterization of an enantioselective arylacetonitrilase from Pseudomonas putida (2006), Arch. Microbiol., 184, 407-418.
    View publication on PubMed
Search for more literature for 3.5.5.5

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Pseudomonas putida
Co2+
-
 Pseudomonas putida
Cu2+
-
 Pseudomonas putida
Fe2+
-
 Pseudomonas putida
Hg2+
-
 Pseudomonas putida
Ni2+
-
 Pseudomonas putida
Zn2+
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.61
-
 phenyl acetonitrile 35°C, pH 7.5 Pseudomonas putida
5.31
-
 indole 3-acetonitrile 35°C, pH 7.5 Pseudomonas putida
13.4
-
 mandelonitrile 35°C, pH 7.5 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 9 or 10 * 43000, SDS-PAGE Pseudomonas putida
412000
-
 gel filtration Pseudomonas putida

Organic Solvent Stability

Organic Solvent Comment Organism
dimethyl sulfoxide 2.5 or 5%, up to 20% increase in activity, decrease above Pseudomonas putida
Ethanol 5%, 63% enhancement in activity probably due to increased availibaility of substrate. Above 10% ethanol, significant decrease in activity Pseudomonas putida
Methanol 5%, 78% enhancement in activity probably due to increased availibaility of substrate. Above 10% methanol, significant decrease in activity Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.26
-
 35°C, pH 7.5 Pseudomonas putida

Storage Stability

Storage Stability Organism
-80°C, 0.01 M phosphate, pH 7.5, 1 mM dithiothreitol, 20% glycerol, stable Pseudomonas putida
4°C, 0.01 M phosphate, pH 7.5, 1 mM dithiothreitol, rapid loss of activity, presence of 20% glycerol stabilizes Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chlorophenyl acetonitrile + H2O 76.1% of the activity with mandelonitrile Pseudomonas putida 2-chlorobenzoic acid + NH3
-
 ir
2-methoxyphenyl acetonitrile + H2O 44.5% of the activity with mandelonitrile Pseudomonas putida 2-methoxybenzoic acid + NH3
-
 ir
2-thiophene acetonitrile + H2O 137% of the activity with mandelonitrile Pseudomonas putida thiophene-2-carboxylic acid + NH3
-
 ir
4-aminophenyl acetonitrile + H2O 502.3% of the activity with mandelonitrile Pseudomonas putida 4-aminobenzoic acid + NH3
-
 ir
4-chlorophenyl acetonitrile + H2O 360.3% of the activity with mandelonitrile Pseudomonas putida 4-chlorobenzoic acid + NH3
-
 ir
4-hydroxy benzonitrile + H2O 23.1% of the activity with mandelonitrile Pseudomonas putida 4-hydroxybenzoate + NH3
-
 ir
4-hydroxyphenyl acetonitrile + H2O 435% of the activity with mandelonitrile Pseudomonas putida 4-hydroxybenzoic acid + NH3
-
 ir
adiponitrile + H2O 17.2% of the activity with mandelonitrile Pseudomonas putida adipic acid + NH3
-
 ir
cyclohexane carbonitrile + H2O 12.5% of the activity with mandelonitrile Pseudomonas putida cyclohexlycarboxylic acid + NH3
-
 ir
hex-5-enenitrile + H2O 7.9% of the activity with mandelonitrile Pseudomonas putida hex-5-enoic acid + NH3
-
 ir
indole 3-acetonitrile + H2O 319.9% of the activity with mandelonitrile Pseudomonas putida (indol-3-yl)acetic acid + NH3
-
 ir
isovaleronitrile + H2O 11.4% of the activity with mandelonitrile Pseudomonas putida isovaleric acid + NH3
-
 ir
mandelonitrile + 2 H2O
-
 Pseudomonas putida (R)-mandelic acid + NH3 99.9% enantiomeric excess for (R)-isomer ir
phenoxy acetonitrile + H2O 232.6% of the activity with mandelonitrile Pseudomonas putida phenoxyacetate + NH3
-
 ir
phenyl acetonitrile + H2O 342.3% of the activity with mandelonitrile Pseudomonas putida benzoic acid + NH3
-
 ir
phenyl glycinenitrile + H2O 192.5% of the activity with mandelonitrile Pseudomonas putida phenylalanine + NH3
-
 ir
phenyl thioacetonitrile + H2O 187.7% of the activity with mandelonitrile Pseudomonas putida thiophenylacetate + NH3
-
 ir
phenylacetonitrile + 2 H2O
-
 Pseudomonas putida phenylacetate + NH3
-
 ir

Subunits

Subunits Comment Organism
decamer 9 or 10 * 43000, SDS-PAGE Pseudomonas putida
nonamer 9 or 10 * 43000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pseudomonas putida

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20
-
 21% of maximum activity Pseudomonas putida
70
-
 11% of maximum activity Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 6 h, 87% residual activity Pseudomonas putida
40
-
 half-life 76 min Pseudomonas putida
50
-
 half-life 9 min Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
 phenyl acetonitrile 35°C, pH 7.5 Pseudomonas putida
11.8
-
 mandelonitrile 35°C, pH 7.5 Pseudomonas putida
13.4
-
 indole 3-acetonitrile 35°C, pH 7.5 Pseudomonas putida
18.85
-
 phenyl acetonitrile 35°C, pH 7.5 Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
 half-life 36 min Pseudomonas putida
7
-
 half-life 110 min Pseudomonas putida
7.5
-
 half-life 87 min Pseudomonas putida
8
-
 half-life 53 min Pseudomonas putida