Improvement of the amides forming capacity of the arylacetonitrilase from Pseudomonas fluorescens EBC191 by site-directed mutagenesis

Sosedov, O.; Stolz, A.; Appl. Microbiol. Biotechnol. 99, 2623-2635 (2015)

Data extracted from this reference:

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

(R,S)-2-phenylpropionitrile + H2O

-

733193

Pseudomonas fluorescens

(S)-2-phenylpropionic acid + NH3

ee 66%

-

-

?

(R,S)-2-phenylpropionitrile + H2O

-

733193

Pseudomonas fluorescens EBC191

(S)-2-phenylpropionic acid + NH3

ee 66%

-

-

?

(R,S)-mandelonitrile + H2O

-

733193

Pseudomonas fluorescens

(R)-mandelic acid + NH3

ee 22%

-

-

?

(R,S)-mandelonitrile + H2O

-

733193

Pseudomonas fluorescens EBC191

(R)-mandelic acid + NH3

ee 22%

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

(R,S)-2-phenylpropionitrile + H2O

-

733193

Pseudomonas fluorescens

(S)-2-phenylpropionic acid + NH3

ee 66%

-

-

?

(R,S)-2-phenylpropionitrile + H2O

-

733193

Pseudomonas fluorescens EBC191

(S)-2-phenylpropionic acid + NH3

ee 66%

-

-

?

(R,S)-mandelonitrile + H2O

-

733193

Pseudomonas fluorescens

(R)-mandelic acid + NH3

ee 22%

-

-

?

(R,S)-mandelonitrile + H2O

-

733193

Pseudomonas fluorescens EBC191

(R)-mandelic acid + NH3

ee 22%

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

733193

Sosedov

Improvement of the amides form ...

Pseudomonas fluorescens, Pseudomonas fluorescens EBC191

Appl. Microbiol. Biotechnol.

99

2623-2635

2015

-

-

1

-

1

-

-

-

-

-

-

-

-

11

-

-

-

-

-

-

-

-

4

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

733558

Sun

Cloning, purification and eval ...

Luminiphilus syltensis, Luminiphilus syltensis NOR5-1B

Biotechnol. Lett.

37

1655-1661

2015

-

-

1

-

-

-

-

1

-

-

1

-

-

4

-

-

1

-

-

-

-

-

41

1

2

1

1

1

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

1

-

-

-

-

41

1

1

1

1

-

1

-

-

-

-

-

-

-

-

-

733546

Bhatia

Purification and characterizat ...

Alcaligenes sp., Alcaligenes sp. MTCC 10675

Biotechnol. Appl. Biochem.

61

459-465

2014

-

-

-

-

-

1

10

1

-

1

2

-

-

3

-

-

1

-

-

-

-

-

8

1

2

1

-

1

1

1

-

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-

-

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-

-

-

-

-

-

1

-

10

-

1

-

1

2

-

-

-

-

1

-

-

-

-

8

1

1

-

1

1

1

-

-

-

-

-

-

-

-

-

718648

Baum

Conversion of sterically deman ...

Pseudomonas fluorescens, Pseudomonas fluorescens EBC191

Appl. Environ. Microbiol.

78

48-57

2012

-

1

1

-

11

-

-

1

-

-

-

2

-

10

-

-

-

-

-

-

-

-

14

-

1

1

-

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2

-

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-

-

1

1

-

-

11

-

-

-

-

1

-

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2

-

-

-

-

-

-

-

-

14

-

1

-

-

-

2

-

-

-

-

-

-

-

-

-

718670

Petrickova

Purification and characterizat ...

Aspergillus niger, Aspergillus niger CBS 513.88, Neurospora crassa, Neurospora crassa OR74A

Appl. Microbiol. Biotechnol.

93

1553-1561

2012

-

-

2

-

-

-

-

6

-

-

-

6

-

7

-

-

2

-

-

-

-

-

24

-

-

2

-

8

-

2

-

9

-

-

-

-

-

-

3

-

-

-

-

-

-

-

9

-

-

-

6

-

-

-

3

-

-

-

-

24

-

3

-

12

-

3

-

14

-

-

1

1

-

-

-

734496

Petrickova

-

Influence of point mutations n ...

Aspergillus niger, Aspergillus niger CBS 513.88, Neurospora crassa, Neurospora crassa DSM 1257

J. Mol. Catal. B

77

74-80

2012

-

-

2

-

4

-

-

-

-

-

-

-

-

5

-

-

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8

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4

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4

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2

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4

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-

-

-

-

-

-

8

-

-

-

-

-

2

4

-

-

-

-

-

-

-

-

719297

Detzel

-

Autodisplay of nitrilase from ...

Alcaligenes faecalis, Alcaligenes faecalis ATCC 8750

ChemCatChem

3

719-725

2011

-

-

1

-

-

-

-

-

-

-

-

-

-

4

-

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2

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1

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1

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1

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2

-

1

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-

-

1

-

-

-

-

-

-

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-

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719629

Liu

Gene cloning, expression, and ...

Alcaligenes faecalis, Alcaligenes faecalis ZJUTB10

J. Agric. Food Chem.

59

11560-11570

2011

-

1

1

-

3

-

11

1

-

2

1

2

-

2

-

-

1

1

-

-

-

-

12

2

-

1

1

1

-

1

-

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-

-

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-

1

1

-

-

3

-

-

11

-

1

-

2

1

2

-

-

-

1

-

-

-

-

12

2

1

1

1

-

1

-

-

-

-

1

1

-

-

-

710940

Sosedov

Construction and application o ...

Pseudomonas fluorescens

Appl. Environ. Microbiol.

76

3668-3674

2010

-

-

-

-

14

-

-

-

-

-

-

-

-

5

-

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2

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-

-

14

-

-

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-

2

-

-

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-

-

-

-

-

-

-

-

-

-

-

-

712643

He

Biocatalytic synthesis of (R)- ...

Alcaligenes faecalis

J. Ind. Microbiol. Biotechnol.

37

741-750

2010

-

-

-

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-

-

-

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4

-

2

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5

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4

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-

5

-

-

-

-

-

-

-

-

-

1

-

-

1

-

-

710930

Kiziak

Identification of amino acid r ...

Pseudomonas fluorescens

Appl. Environ. Microbiol.

75

5592-5599

2009

-

-

1

-

8

-

-

-

-

-

-

-

-

5

-

-

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-

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2

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1

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1

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8

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2

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-

-

-

-

712260

Thuku

Microbial nitrilases: Versatil ...

Alcaligenes faecalis, Alcaligenes faecalis ATCC 8750

J. Appl. Microbiol.

106

703-727

2009

-

-

-

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2

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4

-

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6

1

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1

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1

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2

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6

1

1

-

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-

1

-

-

-

-

-

-

-

-

-

713073

Howden

Pseudomonas syringae pv. syrin ...

no activity in no activity in Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. syringae, Pseudomonas syringae pv. syringae B728a

Mol. Plant Pathol.

10

857-865

2009

-

-

-

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-

-

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-

10

-

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4

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4

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-

717051

Sosedov

-

Synthesis of enantiomerically ...

Pseudomonas fluorescens, Pseudomonas fluorescens EBC191

Adv. Synth. Catal.

351

1531 - 1538

2009

-

-

1

-

-

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5

-

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2

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1

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-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

684655

Rustler

Simultaneous expression of an ...

Pseudomonas fluorescens, Pseudomonas fluorescens EBC191

Appl. Microbiol. Biotechnol.

80

87-97

2008

-

-

1

-

-

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-

-

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13

-

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2

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-

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-

1

1

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1

-

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-

2

-

-

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-

1

1

-

-

-

-

-

-

-

687882

Zhu

A new nitrilase from Bradyrhiz ...

Bradyrhizobium japonicum, Bradyrhizobium japonicum USDA 110

J. Biotechnol.

133

327-333

2008

-

-

1

-

-

-

-

1

-

-

3

-

-

24

-

-

1

-

-

-

-

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26

1

1

1

-

1

1

1

1

-

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-

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-

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-

1

-

-

-

-

-

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1

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3

-

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-

1

-

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-

-

26

1

1

-

1

1

1

1

-

-

-

-

-

-

-

-

694107

Banerjee

enantioselective nitrilase fro ...

Pseudomonas putida, Pseudomonas putida MTCC 5110

Mol. Biotechnol.

41

35-41

2008

-

1

1

-

-

-

-

-

-

-

1

2

-

2

-

-

1

-

-

-

10

-

2

-

2

1

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-

1

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-

1

1

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-

1

2

-

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-

1

-

-

10

-

2

-

1

-

-

-

1

-

-

-

-

-

-

-

-

-

700670

Agerbirk

Sinapis phylogeny and evolutio ...

Sinapis alba

Phytochemistry

69

2937-2949

2008

-

-

-

-

-

-

-

-

-

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1

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3

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-

-

-

3

-

-

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-

-

-

-

-

-

-

-

-

-

-

701254

Rustler

Characterisation of the substr ...

Exophiala oligosperma, Exophiala oligosperma R1

Stud. Mycol.

61

165-174

2008

1

-

-

-

-

-

-

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2

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12

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1

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12

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-

689852

Kiziak

Influence of different carboxy ...

Pseudomonas fluorescens, Pseudomonas fluorescens EBC191

Protein Eng. Des. Sel.

20

385-396

2007

-

-

1

-

1

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10

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1

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4

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1

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1

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1

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4

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-

-

667345

Banerjee

Purification and characterizat ...

Pseudomonas putida

Arch. Microbiol.

184

407-418

2006

-

-

-

-

-

-

7

3

-

-

2

-

3

2

-

-

1

-

-

-

1

2

18

2

-

1

2

3

4

1

-

4

-

-

-

-

-

-

-

-

-

-

-

-

7

-

3

-

-

2

-

3

-

-

1

-

-

1

2

18

2

1

2

3

4

1

-

4

-

-

-

-

-

-

-

718003

Mateo

-

Stabilisation of oxygen-labile ...

Pseudomonas fluorescens, Pseudomonas fluorescens EBC191

J. Mol. Catal. B

38

154-157

2006

-

-

-

-

-

1

-

-

-

-

-

-

-

5

1

-

-

-

-

-

1

-

2

-

-

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-

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-

-

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1

-

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-

-

-

-

1

-

-

-

-

1

-

2

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

718410

Mateo

-

Synthesis of enantiomerically ...

Pseudomonas fluorescens, Pseudomonas fluorescens EBC191

Tetrahedron Asymmetry

17

320-323

2006

-

-

-

-

-

-

-

-

-

-

-

-

-

5

-

-

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-

-

-

-

-

2

-

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-

-

2

-

-

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-

-

-

-

-

-

-

1

1

-

-

-

670163

Kiziak

Nitrilase from Pseudomonas flu ...

Pseudomonas fluorescens, Pseudomonas fluorescens EBC191

Microbiology

151

3639-3648

2005

-

-

1

-

-

1

-

-

-

-

1

-

-

10

-

-

-

-

-

-

-

2

15

1

-

1

-

1

-

1

-

-

-

-

-

-

-

-

1

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

2

15

1

1

-

1

-

1

-

-

-

-

-

-

-

-

-

652959

Layh

-

Characterization and partial p ...

Pseudomonas fluorescens

J. Mol. Catal. B

5

467-474

1998

2

-

-

-

-

-

5

2

-

1

4

-

-

1

-

-

1

-

-

-

1

-

4

1

1

1

-

-

-

1

-

-

-

-

-

-

2

-

-

-

-

-

-

-

5

-

2

-

1

4

-

-

-

-

1

-

-

1

-

4

1

1

-

-

-

1

-

-

-

-

-

-

-

-

-

32502

Mauger

-

Occurrence of a novel nitrilas ...

Alcaligenes faecalis, Alcaligenes faecalis JM3

Arch. Microbiol.

155

1-6

1990

2

-

-

-

-

-

-

-

-

1

-

1

-

4

-

-

-

-

-

-

-

-

20

-

-

1

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

20

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

32503

Nagasawa

A novel nitrilase, arylacetoni ...

Alcaligenes faecalis, Alcaligenes faecalis JM3

Eur. J. Biochem.

194

765-772

1990

3

-

-

-

-

-

27

13

-

-

5

1

1

5

-

-

1

-

-

-

1

4

33

1

-

2

1

3

-

1

1

-

-

-

-

-

3

-

-

-

-

-

-

-

27

-

13

-

-

5

1

1

-

-

1

-

-

1

4

33

1

2

1

3

-

1

1

-

-

-

-

-

-

-

-