BRENDA - Enzyme Database show
show all sequences of 3.5.5.4

A conserved mechanism for nitrile metabolism in bacteria and plants

Howden, A.J.; Harrison, C.J.; Preston, G.M.; Plant J. 57, 243-253 (2009)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
3-Cyano-L-alanine
induction
Pseudomonas fluorescens
cyanide
induction
Pseudomonas fluorescens
cysteine
induction
Pseudomonas fluorescens
Engineering
Amino acid exchange
Commentary
Organism
additional information
PinA is able to complement a nit4 mutation in Arabidopsis thaliana. Overexpression of PinA in wild-type Arabidopsis thaliana results in increased growth in high concentrations of 3-cyano-L-alanine and in increased root elongation in the absence of exogenous 3-cyano-L-alanine
Pseudomonas fluorescens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas fluorescens
-
-
-
Pseudomonas fluorescens SBW25
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-cyano-L-alanine + H2O
-
700754
Pseudomonas fluorescens
L-aspartate + NH3
-
-
-
?
3-cyano-L-alanine + H2O
-
700754
Pseudomonas fluorescens SBW25
L-aspartate + NH3
-
-
-
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
3-Cyano-L-alanine
induction
Pseudomonas fluorescens
cyanide
induction
Pseudomonas fluorescens
cysteine
induction
Pseudomonas fluorescens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
PinA is able to complement a nit4 mutation in Arabidopsis thaliana. Overexpression of PinA in wild-type Arabidopsis thaliana results in increased growth in high concentrations of 3-cyano-L-alanine and in increased root elongation in the absence of exogenous 3-cyano-L-alanine
Pseudomonas fluorescens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-cyano-L-alanine + H2O
-
700754
Pseudomonas fluorescens
L-aspartate + NH3
-
-
-
?
3-cyano-L-alanine + H2O
-
700754
Pseudomonas fluorescens SBW25
L-aspartate + NH3
-
-
-
?
Other publictions for EC 3.5.5.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734737
O'Leary
Increased beta-cyanoalanine ni ...
Pseudomonas fluorescens
Mol. Plant
7
231-243
2014
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
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1
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1
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1
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-
1
-
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-
-
-
-
-
-
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-
1
1
-
-
-
734937
Machingura
The beta-cyanoalanine pathway ...
Arabidopsis thaliana
Plant Physiol. Biochem.
63
159-169
2013
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-
-
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1
-
1
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1
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1
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1
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1
1
-
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-
704926
Gupta
Enzymatic mechanism and bioche ...
Bacillus megaterium, Chromobacterium violaceum, Escherichia coli
J. Hazard. Mater.
176
1-13
2010
-
3
-
-
-
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3
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3
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3
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-
700754
Howden
A conserved mechanism for nitr ...
Pseudomonas fluorescens, Pseudomonas fluorescens SBW25
Plant J.
57
243-253
2009
3
-
-
-
1
-
-
-
-
-
-
-
-
2
-
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-
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2
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3
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1
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2
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713231
Janowitz
Evolution of nitrilases in glu ...
Allium cepa, Arabidopsis thaliana, Brassica rapa, Capsella rubella, Carica papaya, Curcuma longa, Glycine max, Hordeum vulgare, Lactuca sativa, Linum usitatissimum, Lotus japonicus, Lupinus angustifolius, Manihot esculenta, Medicago truncatula, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Pinus taeda, Populus trichocarpa, Ricinus communis, Saccharum officinarum, Selaginella moellendorffii, Solanum lycopersicum, Sorghum bicolor, Tarenaya spinosa, Triticum aestivum, Tropaeolum majus, Vitis vinifera, Zea mays
Phytochemistry
70
1680-1686
2009
-
-
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-
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-
32
-
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32
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32
-
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-
688087
Kriechbaumer
Maize nitrilases have a dual r ...
Zea mays
J. Exp. Bot.
58
4225-4233
2007
2
-
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2
1
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1
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1
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1
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2
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1
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2
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1
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1
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1
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2
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1
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689084
Ishikawa
Molecular cloning of Brassica ...
Brassica rapa
Mol. Plant Pathol.
8
623-637
2007
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-
1
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1
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1
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1
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1
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2
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1
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1
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1
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1
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1
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2
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1
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689769
Jenrich
Evolution of heteromeric nitri ...
Sorghum bicolor
Proc. Natl. Acad. Sci. USA
104
18848-18853
2007
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-
1
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-
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2
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2
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5
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1
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2
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670571
Piotrowski
Cyanide metabolism in higher p ...
Lupinus angustifolius
Plant Mol. Biol.
61
111-122
2006
-
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-
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1
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1
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6
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1
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3
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6
1
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2
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2
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2
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4
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6
2
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5824
Yanase
-
Metabolism of nitriles in micr ...
Pseudomonas sp., Pseudomonas sp. 13
Agric. Biol. Chem.
47
473-482
1983
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1
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1
17
1
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2
2
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3
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1
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1
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4
1
1
1
1
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1
1
1
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1
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1
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17
-
1
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2
2
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1
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1
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4
1
1
1
1
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1
1
1
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