Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 CodonPlus (DE3) RIL cells | Sorghum bicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.86 | - |
3-Cyano-L-alanine | isozyme complex NIT4A/B1, in 50 mM potassium phosphate (pH 8.0), 1 mM dithiothreitol, at 37°C | Sorghum bicolor | |
0.89 | - |
3-Cyano-L-alanine | isozyme complex NIT4A/B2, in 50 mM potassium phosphate (pH 8.0), 1 mM dithiothreitol, at 37°C | Sorghum bicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
isozyme NIT4A, SDS-PAGE | Sorghum bicolor |
42000 | - |
isozyme NIT4B2, SDS-PAGE | Sorghum bicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Ni-NTA column chromatography | Sorghum bicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-cyano-L-alanine + H2O | high activity substrate for isozyme complexes NIT4A/B2 and NIT4A/B1 | Sorghum bicolor | L-aspartate + NH3 | - |
ir | |
additional information | recombinant NIT4 isozymes NIT4A, NIT4B1, and NIT4B2 do not hydrolyze 3-cyano-L-alanine, benzonitrile, 4-hydroxybenzonitrile, butyronitrile, and 4-pentenenitrile | Sorghum bicolor | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cyanoalanine hydratase | - |
Sorghum bicolor |
NIT4A/B1 | active isozyme complex | Sorghum bicolor |
NIT4A/B2 | actice isozyme complex | Sorghum bicolor |
nitrilase 4 | - |
Sorghum bicolor |