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Literature summary for 3.5.5.4 extracted from

  • Kriechbaumer, V.; Park, W.J.; Piotrowski, M.; Meeley, R.B.; Gierl, A.; Glawischnig, E.
    Maize nitrilases have a dual role in auxin homeostasis and beta-cyanoalanine hydrolysis (2007), J. Exp. Bot., 58, 4225-4233.
    View publication on PubMed
Search for more literature for 3.5.5.4

Activating Compound

Activating Compound Comment Organism Structure
3-Cyano-L-alanine induces protein expression Zea mays
additional information a 2fold induction by light is observed Zea mays

Inhibitors

Inhibitors Comment Organism Structure
jasmonic acid application of jasmonic acid represses protein expression Zea mays
additional information gibberellic acid, kinetin, abscisic acid, or epi-brassinolide have no obvious influence on activity Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
 3-Cyano-L-alanine
-
 Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
 line B73
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and HiLoad 16/60 Superdex 200 gel filtration Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-cyano-L-alanine + H2O
-
 Zea mays L-aspartate + NH3
-
 ?
additional information does not use indole-3-acetonitrile as a substrate Zea mays ?
-
 ?

Synonyms

Synonyms Comment Organism
NIT1
-
 Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 3-Cyano-L-alanine
-
 Zea mays