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Literature summary for 3.5.5.1 extracted from

  • Ricano-Rodríguez, J.; Ramírez-Lepe, M.
    Purification and characterization of a cyanide-degrading nitrilase from Trichoderma harzianum VSL291 (2015), Turk. J. Biol., 39, 248-257 .
No PubMed abstract available
Search for more literature for 3.5.5.1

Inhibitors

Inhibitors Comment Organism Structure
2-propanol 5% (v/v), about 40% loss of activity, 50% (v/v), about 95% loss of activity Trichoderma harzianum
acetone 30% (v/v), 70% loss of activity Trichoderma harzianum
acetonitrile 5% (v/v), about 50% loss of activity, 50% (v/v), about 95% loss of activity Trichoderma harzianum
Ag+ strong inhibition of nitrilase activity. The effect on KCN (0.02 mmol/l) degradation is tested in the presence of Cu2+ and Ag+ ions (0.025 mmol/l to 1.0 mmol/l) and the enzymatic activity is not affected significantly at 0.025 mmol/l, 0.075 mmol/l, and 0.125 mmol/l concentrations. When both ions are combined, the activity of the enzyme decreases significantly Trichoderma harzianum
Al3+ strong inhibition of nitrilase activity Trichoderma harzianum
butanol 5% (v/v), about 75% loss of activity Trichoderma harzianum
Cu2+ strong inhibition of nitrilase activity. The effect on KCN (0.02 mmol/l) degradation is tested in the presence of Cu2+ and Ag+ ions (0.025 mmol/l to 1.0 mmol/l) and the enzymatic activity is not affected significantly at 0.025 mmol/l, 0.075 mmol/l, and 0.125 mmol/l concentrations. When both ions are combined, the activity of the enzyme decreases significantly Trichoderma harzianum
DTT
-
 Trichoderma harzianum
ethanol 30% (v/v), about 25% loss of activity Trichoderma harzianum
H2O2 strong inhibition of nitrilase activity Trichoderma harzianum
hexane 50% (v/v), 60% loss of activity Trichoderma harzianum
methanol 30% (v/v), about 25% loss of activity Trichoderma harzianum
Toluene 5% (v/v), about 65% loss of activity, 50% (v/v), about 85% loss of activity Trichoderma harzianum
Zn2+ strong inhibition of nitrilase activity Trichoderma harzianum

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Trichoderma harzianum 5622
-

Organism

Organism UniProt Comment Textmining
Trichoderma harzianum
-
-
-
Trichoderma harzianum VSL291
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trichoderma harzianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzonitrile + 2 H2O
-
 Trichoderma harzianum benzoic acid + NH3
-
 ?
benzonitrile + 2 H2O
-
 Trichoderma harzianum VSL291 benzoic acid + NH3
-
 ?
potassium cyanide + H2O
-
 Trichoderma harzianum potassium carbonate + NH3
-
 ?
potassium cyanide + H2O
-
 Trichoderma harzianum VSL291 potassium carbonate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
NIT1
-
 Trichoderma harzianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Trichoderma harzianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Trichoderma harzianum

pH Range

pH Minimum pH Maximum Comment Organism
6 8 the enzyme retains more than 80% of its activity between pH 6.0 and pH 8.0 Trichoderma harzianum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 24 h, complete loss of activity Trichoderma harzianum
4.5 8 24 h, less than 20% loss of activity Trichoderma harzianum
8.5
-
 24 h, about 30% loss of activity Trichoderma harzianum
9
-
 24 h, complete loss of activity Trichoderma harzianum