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Literature summary for 3.5.5.1 extracted from

  • Cai, W.; Su, E.; Zhu, S.; Ren, Y.; Ren, Y.; Wei, D.
    Characterization of a nitrilase from Arthrobacter aurescens cyc705 for synthesis of iminodiacetic acid (2015), J. Gen. Appl. Microbiol., 60, 207-214 .
No PubMed abstract available
Search for more literature for 3.5.5.1

Cloned(Commentary)

Cloned (Comment) Organism
a recombinant Escherichia coli BL21(DE3)/pET28a-cyc705 was constructed to achieve the heterologous expression of cyc705 Paenarthrobacter aurescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
43.17
-
 iminodiacetonitrile pH 7.2, 30°C Paenarthrobacter aurescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36700
-
 SDS-PAGE, monmomer Paenarthrobacter aurescens
520000
-
 gel filtration Paenarthrobacter aurescens

Organism

Organism UniProt Comment Textmining
Paenarthrobacter aurescens S5M6X9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paenarthrobacter aurescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-cyanopyridine + 2 H2O activity is 3.27% compared to activity with iminodiacetonitrile Paenarthrobacter aurescens pyridine 2-carboxylic acid + NH3
-
 ?
3-hydroxyglutaronitrile + 4 H2O activity is 1.15% compared to activity with iminodiacetonitrile Paenarthrobacter aurescens 3-hydroxyglutaric acid + 2 NH3
-
 ?
3-hydroxypropionitrile + H2O activity is 2.57% compared to activity with iminodiacetonitrile Paenarthrobacter aurescens 3-hydroxypropanoate + NH3
-
 ?
3-phenylpropionitrile + H2O activity is 7.24% compared to activity with iminodiacetonitrile Paenarthrobacter aurescens 3-phenylpropanoate + NH3
-
 ?
4-chlorobutyronitrile + H2O activity is 11.1% compared to activity with iminodiacetonitrile Paenarthrobacter aurescens 4-chlorobutyrate + NH3
-
 ?
4-cyanopyridine + H2O activity is 2.48% compared to activity with iminodiacetonitrile Paenarthrobacter aurescens pyridine 4-carboxylic acid + NH3
-
 ?
adiponitrile + H2O activity is 27.1% compared to activity with iminodiacetonitrile Paenarthrobacter aurescens adipic acid + NH3
-
 ?
cinnamonitrile + H2O activity is 7.87% compared to activity with iminodiacetonitrile Paenarthrobacter aurescens cinnamic acid + NH3
-
 ?
iminodiacetonitrile + H2O
-
 Paenarthrobacter aurescens iminodiacetic acid + NH3
-
 ?
malononitrile + H2O activity is 78.9% compared to activity with iminodiacetonitrile Paenarthrobacter aurescens malonic acid + NH3
-
 ?
mandelonitrile + H2O activity is 2.38% compared to activity with iminodiacetonitrile Paenarthrobacter aurescens mandelic acid + NH3
-
 ?
additional information the suitable substrates for the purified nitrilase were short-chain aliphatic dinitriles Paenarthrobacter aurescens ?
-
-
succinonitrile + H2O activity is 104.8% compared to activity with iminodiacetonitrile Paenarthrobacter aurescens succinate + NH3
-
 ?
valeronitrile + H2O activity is 4.9% compared to activity with iminodiacetonitrile Paenarthrobacter aurescens valeric acid + NH3
-
 ?

Subunits

Subunits Comment Organism
tetradecamer 14 * 36700, SDS-PAGE Paenarthrobacter aurescens

Synonyms

Synonyms Comment Organism
cyc705
-
 Paenarthrobacter aurescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Paenarthrobacter aurescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.57
-
 iminodiacetonitrile pH 7.2, 30°C Paenarthrobacter aurescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
 Paenarthrobacter aurescens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.036
-
 iminodiacetonitrile pH 7.2, 30°C Paenarthrobacter aurescens