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Literature summary for 3.5.5.1 extracted from

  • Liu, D.; Xi, L.; Han, D.; Dou, K.; Su, S.; Liu, J.
    Cloning, expression, and characterization of a novel nitrilase, PaCNit, from Pannonibacter carbonis Q4.6 (2019), Biotechnol. Lett., 41, 583-589 .
    View publication on PubMed
Search for more literature for 3.5.5.1

Activating Compound

Activating Compound Comment Organism Structure
acetone 5% (v/v), 1.2fold activation Pannonibacter carbonis
dithiothreitol 1 mM, 1.2fold activation Pannonibacter carbonis
EDTA 1 mM, 1.35fold activation Pannonibacter carbonis
glycerin 1% (v/v), 1.3fold activation Pannonibacter carbonis
L-cysteine 1 mM, 1.3fold activation Pannonibacter carbonis
L-glutathione 1 mM, 1.3fold activation Pannonibacter carbonis
SDS 1% (v/v), 1.4fold activation Pannonibacter carbonis
Span-80 1% (v/v), 1.2fold activation Pannonibacter carbonis

Application

Application Comment Organism
synthesis the broad range of substrate specificity, the high activity towards aliphatic, aromatic, and arylacetonitriles, and the highly stability, in terms of temperature, pH, and metal ions make the enzyme a promising biocatalyst for mild nitrile hydrolysis Pannonibacter carbonis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Pannonibacter carbonis

Inhibitors

Inhibitors Comment Organism Structure
BaCl2 5 mM, 35% inhibition Pannonibacter carbonis
CaCl2 5 mM, 34% inhibition Pannonibacter carbonis
dimethyl sulfoxide 1% (v/v) Pannonibacter carbonis
dithiothreitol 5 mM, 37% inhibition Pannonibacter carbonis
DMSO 5% (v/v), % inhibition Pannonibacter carbonis
ether 5% (v/v), 23% inhibition Pannonibacter carbonis
ethyl acetate 5% (v/v), 35% inhibition Pannonibacter carbonis
FeCl3 5 mM, 23% inhibition Pannonibacter carbonis
FeSO4 5 mM, 47% inhibition Pannonibacter carbonis
glycerin 5% (v/v), 52% inhibition Pannonibacter carbonis
KCl 5 mM, 58% inhibition Pannonibacter carbonis
methanol 5% (v/v), 29% inhibition Pannonibacter carbonis
MgCl2 5 mM, 18% inhibition Pannonibacter carbonis
n-heptane 5% (v/v), 25% inhibition Pannonibacter carbonis
NaCl 5 mM, 26% inhibition Pannonibacter carbonis
SDS 5% (v/v), 37% inhibition Pannonibacter carbonis
Triton X-100 5% (v/v), 14% inhibition Pannonibacter carbonis
ZnCl2 5 mM, 32% inhibition Pannonibacter carbonis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10.31
-
 3-Cyanopyridine pH 7.0, 65°C Pannonibacter carbonis

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ 1 mM, 3.2fold activation Pannonibacter carbonis
Cr3+ 5 mM, 2.8fold activation Pannonibacter carbonis
Cu2+ 5 mM, 1.3fold activation Pannonibacter carbonis
Hg2+ 5 mM, 5.2fold activation Pannonibacter carbonis
Ni2+ 5 mM, 3fold activation Pannonibacter carbonis

Organism

Organism UniProt Comment Textmining
Pannonibacter carbonis
-
-
-
Pannonibacter carbonis Q4.6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pannonibacter carbonis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylglutaronitrile + H2O
-
 Pannonibacter carbonis ? + NH3
-
 ?
3-cyanopyridine + 2 H2O
-
 Pannonibacter carbonis nicotinic acid + NH3
-
 ?
4-chlorobutyronitrile + H2O
-
 Pannonibacter carbonis 4-chlorobutyrate + NH3
-
 ?
acetonitrile + H2O
-
 Pannonibacter carbonis acetic acid + NH3
-
 ?
acrylonitrile + H2O
-
 Pannonibacter carbonis acrylic acid + NH3
-
 ?
benzonitrile + 2 H2O
-
 Pannonibacter carbonis benzoic acid + NH3
-
 ?
dicyanobutane + H2O
-
 Pannonibacter carbonis ? + NH3
-
 ?
dicyanooctane + H2O
-
 Pannonibacter carbonis ? + NH3
-
 ?
dodecanenitrile + H2O
-
 Pannonibacter carbonis dodecanoic acid + NH3
-
 ?
fumaronitrile + H2O
-
 Pannonibacter carbonis fumaric acid + NH3
-
 ?
iminodiacetonitrile + H2O
-
 Pannonibacter carbonis iminodiacetic acid + NH3
-
 ?
indole-3-acetonitrile + H2O
-
 Pannonibacter carbonis indol-3-acetic acid + NH3
-
 ?
mandelonitrile + H2O
-
 Pannonibacter carbonis mandelic acid + NH3
-
 ?
pentanenitrile + H2O
-
 Pannonibacter carbonis pentanoic acid + NH3
-
 ?
phenylacetonitrile + 2 H2O
-
 Pannonibacter carbonis phenylacetic acid + NH3
-
 ?
phenylenediacetonitrile + H2O
-
 Pannonibacter carbonis ? + NH3
-
 ?
phenylpropionitrile + 2 H2O
-
 Pannonibacter carbonis phenylpropionic acid + NH3
-
 ?
succinonitrile + H2O
-
 Pannonibacter carbonis succinic acid + NH3
-
 ?
thiophene + H2O
-
 Pannonibacter carbonis ? + NH3
-
 ?

Synonyms

Synonyms Comment Organism
PaCNit
-
 Pannonibacter carbonis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Pannonibacter carbonis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 90 30°C: about 60% of maximal activity, 90°C: about 50% of maximal activity Pannonibacter carbonis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pannonibacter carbonis

pH Range

pH Minimum pH Maximum Comment Organism
4 11 pH 4.0: about 40% of maximal activity, pH 11.0: about 50% of maximal activity Pannonibacter carbonis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 relative enzyme activity is retained at pH 6.0 and 7.0 after incubating for 12 h at 4°C Pannonibacter carbonis