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Literature summary for 3.5.5.1 extracted from

  • Gong, J.S.; Li, H.; Zhu, X.Y.; Lu, Z.M.; Wu, Y.; Shi, J.S.; Xu, Z.H.
    Fungal His-tagged nitrilase from Gibberella intermedia: gene cloning, heterologous expression and biochemical properties (2012), PLoS ONE, 7, e50622.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.5.1

Activating Compound

Activating Compound Comment Organism Structure
EDTA 114% activity at 1 mM Fusarium proliferatum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta-gami (DE3) cells Fusarium proliferatum

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 1 mM Fusarium proliferatum
Ba2+ about 76% residual activity at 1 mM Fusarium proliferatum
Ca2+ about 77% residual activity at 1 mM Fusarium proliferatum
Co2+ 1 mM decreases the enzyme activity to about 90% Fusarium proliferatum
Cu2+ about 75% residual activity at 1 mM Fusarium proliferatum
ethanol a relative activity of 49% is detected in 5% (v/v) of ethanol Fusarium proliferatum
Fe2+ about 79% residual activity at 1 mM Fusarium proliferatum
Li+ about 70% residual activity at 1 mM Fusarium proliferatum
methanol the enzyme is relatively active in 5% (v/v) methanol with 60% of the enzyme activity remaining Fusarium proliferatum
Ni2+ 1 mM decreases the enzyme activity to about 90% Fusarium proliferatum
Pb2+ about 78% residual activity at 1 mM Fusarium proliferatum
Propanol a relative activity of 6% is detected in 5% (v/v) of propanol Fusarium proliferatum
Zn2+ 1 mM decreases the enzyme activity to about 90% Fusarium proliferatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12.11
-
 3-Cyanopyridine at pH 7.2 and 30°C Fusarium proliferatum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 110% activity at 1 mM Fusarium proliferatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35940
-
 x * 35940, calculated from amino acid sequence Fusarium proliferatum
37000
-
 x * 37000, SDS-PAGE Fusarium proliferatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-cyanopyridine + 2 H2O Fusarium proliferatum 100% activity, the enzyme is highly specific towards heterocyclic nitriles such as 3- and 4-cyanopyridine nicotinic acid + NH3
-
 ?
4-cyanopyridine + 2 H2O Fusarium proliferatum 69.25% activity compared to 3-cyanopyridine, the enzyme is highly specific towards heterocyclic nitriles such as 3- and 4-cyanopyridine pyridine 4-carboxylic acid + NH3
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
additional information when 20% (v/v) of organic solvent is used, the enzyme activity sharply decreases and only can be detected with very low activity except that in methanol and 2-chloromethane (40% and 10% of activity retaining, respectively) Fusarium proliferatum

Organism

Organism UniProt Comment Textmining
Fusarium proliferatum
-
-
-
Fusarium proliferatum CA3-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Fusarium proliferatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-3-cyanopyridine + H2O 0.21% activity compared to 3-cyanopyridine Fusarium proliferatum ?
-
 ?
2-chloro-4-cyanopyridine + H2O 7.48% activity compared to 3-cyanopyridine Fusarium proliferatum ?
-
 ?
3-cyanopyridine + 2 H2O 100% activity, the enzyme is highly specific towards heterocyclic nitriles such as 3- and 4-cyanopyridine Fusarium proliferatum nicotinic acid + NH3
-
 ?
4-cyanopyridine + 2 H2O 69.25% activity compared to 3-cyanopyridine, the enzyme is highly specific towards heterocyclic nitriles such as 3- and 4-cyanopyridine Fusarium proliferatum pyridine 4-carboxylic acid + NH3
-
 ?
4-cyanopyridine + 2 H2O 0.07% activity compared to 3-cyanopyridine Fusarium proliferatum pyridine 4-carboxylic acid + NH3
-
 ?
acetonitrile + H2O 2.31% activity compared to 3-cyanopyridine Fusarium proliferatum acetate + NH3
-
 ?
acetonitrile + H2O 2.31% activity compared to 3-cyanopyridine Fusarium proliferatum CA3-1 acetate + NH3
-
 ?
acrylonitrile + H2O 43.96% activity compared to 3-cyanopyridine Fusarium proliferatum acrylic acid + NH3
-
 ?
acrylonitrile + H2O 43.96% activity compared to 3-cyanopyridine Fusarium proliferatum CA3-1 acrylic acid + NH3
-
 ?
adiponitrile + H2O 106.29% activity compared to 3-cyanopyridine Fusarium proliferatum adipic acid + NH3
-
 ?
adiponitrile + H2O 106.29% activity compared to 3-cyanopyridine Fusarium proliferatum CA3-1 adipic acid + NH3
-
 ?
benzonitrile + 2 H2O 42.14% activity compared to 3-cyanopyridine Fusarium proliferatum benzoic acid + NH3
-
 ?
glycinonitrile + H2O 23.27% activity compared to 3-cyanopyridine Fusarium proliferatum ?
-
 ?
iminodiacetonitrile + 2 H2O 58.07% activity compared to 3-cyanopyridine Fusarium proliferatum iminodiacetic acid + 2 NH3
-
 ?
iminodiacetonitrile + 2 H2O 58.07% activity compared to 3-cyanopyridine Fusarium proliferatum CA3-1 iminodiacetic acid + 2 NH3
-
 ?
isovaleronitrile + H2O 47.17% activity compared to 3-cyanopyridine Fusarium proliferatum isovaleric acid + NH3
-
 ?
additional information no activity with mandelonitrile and geranyl nitrile Fusarium proliferatum ?
-
 ?
additional information no activity with mandelonitrile and geranyl nitrile Fusarium proliferatum CA3-1 ?
-
 ?
phenylacetonitrile + 2 H2O 21.59% activity compared to 3-cyanopyridine Fusarium proliferatum phenylacetate + NH3
-
 ?
succinonitrile + H2O 64.22% activity compared to 3-cyanopyridine Fusarium proliferatum succinic acid + NH3
-
 ?
valeronitrile + H2O 45.07% activity compared to 3-cyanopyridine Fusarium proliferatum valeric acid + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Fusarium proliferatum
? x * 35940, calculated from amino acid sequence Fusarium proliferatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Fusarium proliferatum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 the nitrilase activity gradually increases from 20°C to 45°C and is lost rapidly above 50°C Fusarium proliferatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Fusarium proliferatum