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Literature summary for 3.5.5.1 extracted from

  • Rucka, L.; Volkova, O.; Pavlik, A.; Kaplan, O.; Kracik, M.; Ne?vera, J.; Martinkova, L.; Patek, M.
    Expression control of nitrile hydratase and amidase genes in Rhodococcus erythropolis and substrate specificities of the enzymes (2014), Antonie van Leeuwenhoek, 105, 1179-1190.
    View publication on PubMed
Search for more literature for 3.5.5.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, SDS-PAGE Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis B1GY01
-
-
Rhodococcus erythropolis A4 B1GY01
-
-

Purification (Commentary)

Purification (Comment) Organism
ion exchange and hydrophobic and hydrophobic interaction chromatography Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-chlorophenyl)(hydroxy)acetonitrile + H2O
-
 Rhodococcus erythropolis 2-(2-chlorophenyl)-2-hydroxyacetamide
-
 ?
(2-chlorophenyl)(hydroxy)acetonitrile + H2O
-
 Rhodococcus erythropolis A4 2-(2-chlorophenyl)-2-hydroxyacetamide
-
 ?
(2-ethylphenyl)(hydroxy)acetonitrile + H2O
-
 Rhodococcus erythropolis 2-(2-ethylphenyl)-2-hydroxyacetamide
-
 ?
(2-ethylphenyl)(hydroxy)acetonitrile + H2O
-
 Rhodococcus erythropolis A4 2-(2-ethylphenyl)-2-hydroxyacetamide
-
 ?
(3E)-2-hydroxypent-3-enenitrile + H2O
-
 Rhodococcus erythropolis (3E)-2-hydroxypent-3-enamide
-
 ?
(3E)-2-hydroxypent-3-enenitrile + H2O
-
 Rhodococcus erythropolis A4 (3E)-2-hydroxypent-3-enamide
-
 ?
benzamide + H2O
-
 Rhodococcus erythropolis benzoate + NH3
-
 ?
benzamide + H2O
-
 Rhodococcus erythropolis A4 benzoate + NH3
-
 ?
hydroxy(3-phenoxyphenyl)acetonitrile + H2O
-
 Rhodococcus erythropolis 2-hydroxy-2-(3-phenoxyphenyl)acetamide
-
 ?
hydroxy(3-phenoxyphenyl)acetonitrile + H2O
-
 Rhodococcus erythropolis A4 2-hydroxy-2-(3-phenoxyphenyl)acetamide
-
 ?
hydroxy(4-methylphenyl)acetonitrile + H2O
-
 Rhodococcus erythropolis 2-hydroxy-2-(4-methylphenyl)acetamide
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Rhodococcus erythropolis