Literature summary for 3.5.5.1 extracted from

  • Liu, Z.Q.; Zhou, M.; Zhang, X.H.; Xu, J.M.; Xue, Y.P.; Zheng, Y.G.
    Biosynthesis of iminodiacetic acid from iminodiacetonitrile by immobilized recombinant Escherichia coli harboring nitrilase (2012), J. Mol. Microbiol. Biotechnol., 22, 35-47.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme is useful fpr synthesis of iminodiacetic acid, which is widely used as an intermediate in the manufacture of chelating agents, glyphosate herbicides and surfactants Acidovorax facilis

Cloned(Commentary)

Cloned (Comment) Organism
functional expression in Escherichia coli strain BL21 (DE3) Acidovorax facilis

Engineering

Protein Variants Comment Organism
additional information immobilization and stabilization of Escherichia coli cells in 8.0% polyvinyl alcohol and 1.0% sodium alginate copolymer recombinantly expressing the Acidovorax facilis nitrilase for stable production of iminoacetic acid, method optimization, overview. Maximum relative nitrilase activity with 1.0% CaCl2, and 5.0% wet cells, with 1.0% iminodiacetonitrile in distilled water at 40°C. Substrate specificity of immobilized and free cells, overview. Ca2+ can stabilize the cells, but is toxic at concentrations above 1% leading to a sharp decrease in nitrilase activity of immobilized cells. Immobilized and free cells display different sensitivity towards temperatures ranging from 25°C to 70°C, with optimal tempartures of 40°C and 45°C, respectively. Comparison of operational and storage stability of free and immobilized cells, overview Acidovorax facilis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ at concentrations of CaCl2 of 1.0-5.0%, the activity of nitrilase decreases sharply, higher concentration of CaCl 2 seem to have a poisoning effect on the cells Acidovorax facilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000, about, sequence calculation and SDS-PAGE Acidovorax facilis

Organism

Organism UniProt Comment Textmining
Acidovorax facilis Q1W2L4
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
44
-
enzyme activity in immobilized cells, pH and temperature not specified in the publication Acidovorax facilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity of immobilized and free Escherichia coli cells recombinantly expressing the Acidovorax facilis nitrilase, overview. No activity with mandelonitrile, o-methoxyphenylacetonitrile, m-methoxyphenylacetonitrile, p-methoxyphenylacetonitrile, and phenylacetonitrile Acidovorax facilis ?
-
?
iminodiacetonitrile + 2 H2O high activity Acidovorax facilis iminodiacetic acid + 2 NH3
-
?
benzonitrile + 2 H2O
-
Acidovorax facilis benzoic acid + NH3
-
?
acetonitrile + 2 H2O
-
Acidovorax facilis acetic acid + NH3
-
?
acrylonitrile + 2 H2O very low activity Acidovorax facilis acrylic acid + NH3
-
?
adiponitrile + 2 H2O high activity Acidovorax facilis adipic acid + NH3
-
?
2,2-dimethylcyclopropyl cyanide + 2 H2O
-
Acidovorax facilis 2,2-dimethylcyclopropyl carboxylate + NH3
-
?
2-amino-2,3-dimethylbutanenitrile + 2 H2O
-
Acidovorax facilis ? + NH3
-
?
3-hydroxy-glutaronitrile + 2 H2O
-
Acidovorax facilis 3-hydroxy-glutaric acid + NH3
-
?
4-aminobenzonitrile + 2 H2O
-
Acidovorax facilis 4-aminobenzoic acid + NH3
-
?
4-methoxybenzyl cyanide + 2 H2O very low activity Acidovorax facilis 4-methoxyphenylacetic acid + NH3
-
?
2-aminobutyronitrile + 2 H2O high activity Acidovorax facilis 2-aminobutyric acid + NH3
-
?
glycolonitrile + 2 H2O high activity Acidovorax facilis glycolic acid + NH3
-
?

Subunits

Subunits Comment Organism
? x * 43000, about, sequence calculation and SDS-PAGE Acidovorax facilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
in vivo assay, recombinant cells Acidovorax facilis