Cloned (Comment) | Organism |
---|---|
gene nit1, DNA and amino acid sequence determination and analysis, expression of His-tagged Nit1 in Escherichia coli strain BL21(DE3) | uncultured bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
47 | - |
glutaronitrile | pH 7.0, 30°C, recombinant His-tagged Nit1 | uncultured bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, about, SDS-PAGE | uncultured bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | E1A0Z9 | gene nit1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged Nit1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | uncultured bacterium |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | no growth on 4-tolunitrile or 4-hydroxybenzonitrile as sole nitrogen sources | uncultured bacterium | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylglutaronitrile + 2 H2O | regioselectivity of Nit1 | uncultured bacterium | 4-cyanopentanoic acid + NH3 | - |
? | |
3-cyanopyridine + 2 H2O | - |
uncultured bacterium | pyridine 3-carboxylic acid + NH3 | - |
? | |
3-phenylpropionitrile + H2O | - |
uncultured bacterium | 3-phenylpropionic acid + NH3 | - |
? | |
cinnamonitrile + H2O | - |
uncultured bacterium | ? | - |
? | |
glutaronitrile + H2O | - |
uncultured bacterium | glutaric acid + NH3 | - |
? | |
additional information | Nit1 substrate specificity, overview. Significant activities are observed with the aromatic substrates 3-phenylpropionitrile and cinnamonitrile. To a minor degree, the heterocyclic 3-cyanopyridine is also accepted as substrate. In contrast to aliphatic dinitriles, the aliphatic mononitriles acetonitrile, propionitrile, and butyronitrile are almost not converted by Nit1, no activity on malononitrile | uncultured bacterium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, about, SDS-PAGE | uncultured bacterium |
Synonyms | Comment | Organism |
---|---|---|
NIT1 | - |
uncultured bacterium |
nitrilase | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant His-tagged Nit1 | uncultured bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant His-tagged Nit1, 1 h, stable | uncultured bacterium |
50 | - |
purified recombinant His-tagged Nit1, 30 min, loss of 50% activity | uncultured bacterium |
60 | - |
purified recombinant His-tagged Nit1, 10 in, complete inactivation | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant His-tagged Nit1 | uncultured bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | over 50% of maximal activity within this range | uncultured bacterium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
glutaronitrile | pH 7.0, 30°C, recombinant His-tagged Nit1 | uncultured bacterium |