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Literature summary for 3.5.5.1 extracted from

  • Luo, H.; Fan, L.; Chang, Y.; Ma, J.; Yu, H.; Shen, Z.
    Gene cloning, overexpression, and characterization of the nitrilase from Rhodococcus rhodochrous tg1-A6 in E. coli (2008), Appl. Biochem. Biotechnol., 160, 393-400.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned into expression vector pET-28a and expressed in Escherichia coli strain BL21(DE3) Rhodococcus rhodochrous
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) under the control of T7 lac promoter using pET-28a to give pET-Nit, lactose is best as inducer in different media, method optimization Rhodococcus rhodochrous

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
SDS-PAGE Rhodococcus rhodochrous

Organism

Organism UniProt Comment Textmining
Rhodococcus rhodochrous A4LA85
-
-
Rhodococcus rhodochrous tg1-A6 A4LA85
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-cyanopyridine + 2 H2O about 50% activity compared to acrylonitrile Rhodococcus rhodochrous nicotinic acid + NH3
-
?
3-cyanopyridine + 2 H2O about 50% activity compared to acrylonitrile Rhodococcus rhodochrous tg1-A6 nicotinic acid + NH3
-
?
3-cyanopyridine + 2 H2O 50% of the activity with acrylonitrile Rhodococcus rhodochrous pyridine 3-carboxylic acid + NH3
-
?
3-cyanopyridine + 2 H2O 50% of the activity with acrylonitrile Rhodococcus rhodochrous tg1-A6 pyridine 3-carboxylic acid + NH3
-
?
acrylonitrile + 2 H2O best substrate Rhodococcus rhodochrous acrylic acid + NH3
-
?
acrylonitrile + H2O 100% activity, the optimum substrate concentration is 225 mM acrylonitrile Rhodococcus rhodochrous acrylic acid + NH3
-
?
acrylonitrile + H2O 100% activity, the optimum substrate concentration is 225 mM acrylonitrile Rhodococcus rhodochrous tg1-A6 acrylic acid + NH3
-
?
aminoacetonitrile + 2 H2O 25% of the activity with acrylonitrile Rhodococcus rhodochrous aminoacetic acid + NH3
-
?
aminoacetonitrile + H2O about 30% activity compared to acrylonitrile Rhodococcus rhodochrous ?
-
?
glycolonitrile + 2 H2O 40% of the activity with acrylonitrile Rhodococcus rhodochrous glycolic acid + NH3
-
?
glycolonitrile + H2O about 40% activity compared to acrylonitrile Rhodococcus rhodochrous ?
-
?
iminodiacetonitrile + 2 H2O about 25% activity compared to acrylonitrile Rhodococcus rhodochrous iminodiacetic acid + 2 NH3
-
?
iminodiacetonitrile + 2 H2O 20% of the activity with acrylonitrile Rhodococcus rhodochrous iminodiacetic acid + 2 NH3
-
?
iminodiacetonitrile + 2 H2O about 25% activity compared to acrylonitrile Rhodococcus rhodochrous tg1-A6 iminodiacetic acid + 2 NH3
-
?
iminodiacetonitrile + 2 H2O 20% of the activity with acrylonitrile Rhodococcus rhodochrous tg1-A6 iminodiacetic acid + 2 NH3
-
?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Rhodococcus rhodochrous
? x * 42000, recombinant enzyme, SDS-PAGE Rhodococcus rhodochrous

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Rhodococcus rhodochrous
45
-
recombinant nitrilase Rhodococcus rhodochrous

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Rhodococcus rhodochrous
8
-
recombinant nitrilase Rhodococcus rhodochrous