Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.5.1 extracted from

  • Xie, Z.; Feng, J.; Garcia, E.; Bernett, M.; Yazbeck, D.; Tao, J.
    Cloning and optimization of a nitrilase for the synthesis of (3S)-3-cyano-5-methyl hexanoic acid (2006), J. Mol. Catal. B, 41, 75-80.
No PubMed abstract available
Search for more literature for 3.5.5.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strains BL21 (DE3), BRLplys (DE3), Rosetta (DE3), and Tuner (DE3) Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
A200G 37% increased activity compared to the wild type enzyme Arabidopsis thaliana
C236S mutant has a nearly 3fold increase in reactivity Arabidopsis thaliana
H101Q/C239S/D246E 47% increased activity compared to the wild type enzyme Arabidopsis thaliana
I29L 113% increased activity compared to the wild type enzyme Arabidopsis thaliana
P172S/C236S/V291I 84% increased activity compared to the wild type enzyme Arabidopsis thaliana
R89S/G179G 89% increased activity compared to the wild type enzyme Arabidopsis thaliana
T28S 30% increased activity compared to the wild type enzyme Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
440000
-
 gel filtration Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin chromatography and Superdex 75 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S)-isobutylsuccinonitrile + H2O specific for the (3S)-isomer Arabidopsis thaliana (3S)-3-cyano-5-methyl hexanoic acid
-
 ?

Subunits

Subunits Comment Organism
dodecamer gel filtration Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
NIT1
-
 Arabidopsis thaliana