Activating Compound | Comment | Organism | Structure |
---|---|---|---|
indole-3-acetonitrile | induces protein expression | Zea mays | |
additional information | a 2fold induction by light is observed | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
jasmonic acid | application of jasmonic acid represses protein expression | Zea mays | |
additional information | gibberellic acid, kinetin, abscisic acid, or epi-brassinolide have no obvious influence on activity | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
indole-3-acetonitrile | - |
Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
line B73 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and HiLoad 16/60 Superdex 200 gel filtration | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
indole-3-acetonitrile + H2O | preferred substrate of NIT2 | Zea mays | indole-3-acetic acid + NH3 | - |
? | |
additional information | NIT2 does not use 3-cyano-L-alanine as a substrate, however, turnover of 3-cyano-L-alanine function is acquired when NIT2 forms heteromers with the homologue NIT1 | Zea mays | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NIT2 | - |
Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
indole-3-acetonitrile | - |
Zea mays |