Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Urea | 19% increase of activity at 1% (w/v) | Aspergillus niger |
General Stability | Organism |
---|---|
the enzyme stability is markedly improved in the presence of D-sorbitol and xylitol (20% w/v), or myo-inositol (10% w/v) | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 0.1 mM | Aspergillus niger | |
Al2(SO4)3 | complete inhibition at 5 mM | Aspergillus niger | |
ammonium sulfate | 75% residual activity at 800 mM | Aspergillus niger | |
Benzamide | 66% residual activity at 25 mM | Aspergillus niger | |
Ca2+ | 96% residual activity at 5 mM | Aspergillus niger | |
Co2+ | 62% residual activity at 5 mM | Aspergillus niger | |
Cr3+ | 7% residual activity at 5 mM | Aspergillus niger | |
Cu2+ | 1% residual activity at 0.1 mM | Aspergillus niger | |
dithiothreitol | 58% residual activity at 1 mM | Aspergillus niger | |
DL-cysteine | 97% residual activity at 1% (w/v) | Aspergillus niger | |
EDTA | 93% residual activity at 15 mM | Aspergillus niger | |
Fe2+ | 60% residual activity at 5 mM | Aspergillus niger | |
Fe3+ | 71% residual activity at 5 mM | Aspergillus niger | |
H2O2 | 4% residual activity at 5 mM | Aspergillus niger | |
Hg2+ | complete inhibition at 0.1 mM | Aspergillus niger | |
iodoacetamide | 67% residual activity at 1 mM | Aspergillus niger | |
Mg2+ | 91% residual activity at 5 mM | Aspergillus niger | |
NaN3 | 88% residual activity at 5 mM | Aspergillus niger | |
Ni2+ | 44% residual activity at 5 mM | Aspergillus niger | |
p-hydroxymercuribenzoate | complete inhibition at 1 mM | Aspergillus niger | |
Pb2+ | 52% residual activity at 0.1 mM | Aspergillus niger | |
phenylhydrazine | 43% residual activity at 1 mM | Aspergillus niger | |
Zn2+ | complete inhibition at 5 mM | Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38500 | - |
SDS-PAGE, stained with Coomassie Brilliant Blue R-250 | Aspergillus niger |
38560 | - |
predicted protein size | Aspergillus niger |
60000 | - |
SDS-PAGE, stained with silver nitrate | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger K10 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Hi-Prep Sephacryl S-200 gel filtration, and Hi-Prep 16/10 Q-Sepharose column chromatography | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.9 | - |
crude extract, at pH 8.0 and 45°C | Aspergillus niger |
91.6 | - |
after 18.7fold purification, at pH 8.0 and 45°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-2-phenylpropionitrile + H2O | 1% activity compared to benzonitrile | Aspergillus niger | ? | - |
? | |
(R,S)-2-phenylpropionitrile + H2O | 1% activity compared to benzonitrile | Aspergillus niger K10 | ? | - |
? | |
1,3-dicyanobenzene + H2O | 8.4% activity compared to benzonitrile | Aspergillus niger | ? | - |
? | |
1,4-dicyanobenzene + H2O | 79.5% activity compared to benzonitrile | Aspergillus niger | ? | - |
? | |
2 benzonitrile + 3 H2O | 100% activity | Aspergillus niger | benzoic acid + benzamide + NH3 | - |
? | |
2-cyanopyridine + 2 H2O | 14.2% activity compared to benzonitrile | Aspergillus niger | pyridine-2-carboxylic acid + NH3 | - |
? | |
2-phenylacetonitrile + 2 H2O | 10.8% activity compared to benzonitrile | Aspergillus niger | 2-phenylacetic acid + NH3 | - |
? | |
3-chlorobenzonitrile + H2O | 41% activity compared to benzonitrile | Aspergillus niger | 3-chlorobenzoic acid + NH3 | - |
? | |
3-chlorobenzonitrile + H2O | 41% activity compared to benzonitrile | Aspergillus niger K10 | 3-chlorobenzoic acid + NH3 | - |
? | |
3-cyanopyridine + 2 H2O | 32.4% activity compared to benzonitrile | Aspergillus niger | pyridine-3-carboxylic acid + NH3 | - |
? | |
3-hydroxybenzonitrile + H2O | 8.4% activity compared to benzonitrile | Aspergillus niger | 3-hydroxybenzoate + NH3 | - |
? | |
3-tolunitrile + H2O | 5.5% activity compared to benzonitrile | Aspergillus niger | 3-methylbenzoic acid + NH3 | - |
? | |
4-chlorobenzonitrile + H2O | 29.8% activity compared to benzonitrile | Aspergillus niger | 4-chlorobenzoate + NH3 | - |
? | |
4-cyanopyridine + 2 H2O | 410.7% activity compared to benzonitrile | Aspergillus niger | pyridine-4-carboxylic acid + NH3 | - |
? | |
4-tolunitrile + H2O | 3.4% activity compared to benzonitrile | Aspergillus niger | 4-methylbenzoic acid + NH3 | - |
? | |
butyronitrile + H2O | 17.6% activity compared to benzonitrile | Aspergillus niger | butyric acid + NH3 | - |
? | |
butyronitrile + H2O | 17.6% activity compared to benzonitrile | Aspergillus niger K10 | butyric acid + NH3 | - |
? | |
propionitrile + H2O | 6.9% activity compared to benzonitrile | Aspergillus niger | propionic acid + NH3 | - |
? | |
propionitrile + H2O | 6.9% activity compared to benzonitrile | Aspergillus niger K10 | propionic acid + NH3 | - |
? | |
thiophen-2-acetonitrile + H2O | 56.1% activity compared to benzonitrile | Aspergillus niger | thiophen-2-ylacetic acid + NH3 | - |
? | |
valeronitrile + H2O | 19.6% activity compared to benzonitrile | Aspergillus niger | valeric acid + NH3 | - |
? | |
valeronitrile + H2O | 19.6% activity compared to benzonitrile | Aspergillus niger K10 | valeric acid + NH3 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-70 | - |
after five freeze-thaw cycles and subsequent storage for 5 days at -70°C, the activity of the enzyme decreases to about one-fifth of the original activity | Aspergillus niger |
30 | 55 | stable at 30°C, highly active at 48°C (with 92% of maximum activity), but its activity declines sharply at higher temperatures (to 53% and 19% at 50°C and 55°C, respectively), at 30°C and pH 7.2-9.0 the enzyme half-life is about 11 h, at 35°C and 40°C, the half-life decreases to 6.2 h and 2.8 h, respectively | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8.5 | - |
Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | a sharp activity decrease is observed at slightly acidic pH values, only a trace activity being observed at pH 5.5, 70% and 17% of the activity being retained at pH 9 and 10, respectively | Aspergillus niger |