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Literature summary for 3.5.5.1 extracted from

  • Kaplan, O.; Vejvoda, V.; Plihal, O.; Pompach, P.; Kavan, D.; Bojarova, P.; Bezouska, K.; Mackova, M.; Cantarella, M.; Jirku, V.; Kren, V.; Martinkova, L.
    Purification and characterization of a nitrilase from Aspergillus niger K10 (2006), Appl. Microbiol. Biotechnol., 73, 567-575.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Urea 19% increase of activity at 1% (w/v) Aspergillus niger

General Stability

General Stability Organism
the enzyme stability is markedly improved in the presence of D-sorbitol and xylitol (20% w/v), or myo-inositol (10% w/v) Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 0.1 mM Aspergillus niger
Al2(SO4)3 complete inhibition at 5 mM Aspergillus niger
ammonium sulfate 75% residual activity at 800 mM Aspergillus niger
Benzamide 66% residual activity at 25 mM Aspergillus niger
Ca2+ 96% residual activity at 5 mM Aspergillus niger
Co2+ 62% residual activity at 5 mM Aspergillus niger
Cr3+ 7% residual activity at 5 mM Aspergillus niger
Cu2+ 1% residual activity at 0.1 mM Aspergillus niger
dithiothreitol 58% residual activity at 1 mM Aspergillus niger
DL-cysteine 97% residual activity at 1% (w/v) Aspergillus niger
EDTA 93% residual activity at 15 mM Aspergillus niger
Fe2+ 60% residual activity at 5 mM Aspergillus niger
Fe3+ 71% residual activity at 5 mM Aspergillus niger
H2O2 4% residual activity at 5 mM Aspergillus niger
Hg2+ complete inhibition at 0.1 mM Aspergillus niger
iodoacetamide 67% residual activity at 1 mM Aspergillus niger
Mg2+ 91% residual activity at 5 mM Aspergillus niger
NaN3 88% residual activity at 5 mM Aspergillus niger
Ni2+ 44% residual activity at 5 mM Aspergillus niger
p-hydroxymercuribenzoate complete inhibition at 1 mM Aspergillus niger
Pb2+ 52% residual activity at 0.1 mM Aspergillus niger
phenylhydrazine 43% residual activity at 1 mM Aspergillus niger
Zn2+ complete inhibition at 5 mM Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38500
-
SDS-PAGE, stained with Coomassie Brilliant Blue R-250 Aspergillus niger
38560
-
predicted protein size Aspergillus niger
60000
-
SDS-PAGE, stained with silver nitrate Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger K10
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Hi-Prep Sephacryl S-200 gel filtration, and Hi-Prep 16/10 Q-Sepharose column chromatography Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.9
-
crude extract, at pH 8.0 and 45°C Aspergillus niger
91.6
-
after 18.7fold purification, at pH 8.0 and 45°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-2-phenylpropionitrile + H2O 1% activity compared to benzonitrile Aspergillus niger ?
-
?
(R,S)-2-phenylpropionitrile + H2O 1% activity compared to benzonitrile Aspergillus niger K10 ?
-
?
1,3-dicyanobenzene + H2O 8.4% activity compared to benzonitrile Aspergillus niger ?
-
?
1,4-dicyanobenzene + H2O 79.5% activity compared to benzonitrile Aspergillus niger ?
-
?
2 benzonitrile + 3 H2O 100% activity Aspergillus niger benzoic acid + benzamide + NH3
-
?
2-cyanopyridine + 2 H2O 14.2% activity compared to benzonitrile Aspergillus niger pyridine-2-carboxylic acid + NH3
-
?
2-phenylacetonitrile + 2 H2O 10.8% activity compared to benzonitrile Aspergillus niger 2-phenylacetic acid + NH3
-
?
3-chlorobenzonitrile + H2O 41% activity compared to benzonitrile Aspergillus niger 3-chlorobenzoic acid + NH3
-
?
3-chlorobenzonitrile + H2O 41% activity compared to benzonitrile Aspergillus niger K10 3-chlorobenzoic acid + NH3
-
?
3-cyanopyridine + 2 H2O 32.4% activity compared to benzonitrile Aspergillus niger pyridine-3-carboxylic acid + NH3
-
?
3-hydroxybenzonitrile + H2O 8.4% activity compared to benzonitrile Aspergillus niger 3-hydroxybenzoate + NH3
-
?
3-tolunitrile + H2O 5.5% activity compared to benzonitrile Aspergillus niger 3-methylbenzoic acid + NH3
-
?
4-chlorobenzonitrile + H2O 29.8% activity compared to benzonitrile Aspergillus niger 4-chlorobenzoate + NH3
-
?
4-cyanopyridine + 2 H2O 410.7% activity compared to benzonitrile Aspergillus niger pyridine-4-carboxylic acid + NH3
-
?
4-tolunitrile + H2O 3.4% activity compared to benzonitrile Aspergillus niger 4-methylbenzoic acid + NH3
-
?
butyronitrile + H2O 17.6% activity compared to benzonitrile Aspergillus niger butyric acid + NH3
-
?
butyronitrile + H2O 17.6% activity compared to benzonitrile Aspergillus niger K10 butyric acid + NH3
-
?
propionitrile + H2O 6.9% activity compared to benzonitrile Aspergillus niger propionic acid + NH3
-
?
propionitrile + H2O 6.9% activity compared to benzonitrile Aspergillus niger K10 propionic acid + NH3
-
?
thiophen-2-acetonitrile + H2O 56.1% activity compared to benzonitrile Aspergillus niger thiophen-2-ylacetic acid + NH3
-
?
valeronitrile + H2O 19.6% activity compared to benzonitrile Aspergillus niger valeric acid + NH3
-
?
valeronitrile + H2O 19.6% activity compared to benzonitrile Aspergillus niger K10 valeric acid + NH3
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-70
-
after five freeze-thaw cycles and subsequent storage for 5 days at -70°C, the activity of the enzyme decreases to about one-fifth of the original activity Aspergillus niger
30 55 stable at 30°C, highly active at 48°C (with 92% of maximum activity), but its activity declines sharply at higher temperatures (to 53% and 19% at 50°C and 55°C, respectively), at 30°C and pH 7.2-9.0 the enzyme half-life is about 11 h, at 35°C and 40°C, the half-life decreases to 6.2 h and 2.8 h, respectively Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
7.2 8.5
-
Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 10 a sharp activity decrease is observed at slightly acidic pH values, only a trace activity being observed at pH 5.5, 70% and 17% of the activity being retained at pH 9 and 10, respectively Aspergillus niger