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Literature summary for 3.5.5.1 extracted from

  • Yildirim, S.; Ruinatscha, R.; Gross, R.; Wohlgemuth, R.; Kohler, H.E.; Witholt, B.; Schmid, A.
    Selective hydrolysis of the nitrile group of cis-dihydrodiols from aromatic nitriles (2006), J. Mol. Catal. B, 38, 76-83.
No PubMed abstract available
Search for more literature for 3.5.5.1

Application

Application Comment Organism
synthesis biotransformation of trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylonitrile to trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylic acid with isolated enzyme, immobilized enzyme and with recombinant cells containing AtNIT1 Arabidopsis thaliana
synthesis biotransformation of trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylonitrile to trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylic acid with isolated enzyme, immobilized enzyme and with recombinant cells containing AtNIT1 Rhodococcus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana
expression in Escherichia coli Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Rhodococcus sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-1,2-dihydroxy-3-cyanocyclohexa-3,5-diene + H2O
-
 Rhodococcus sp. 5,6-dihydroxycyclohexa-1,3-diene-1-carboxylic acid + NH3
-
 ?
trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylonitrile + H2O
-
 Arabidopsis thaliana trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylic acid + NH3
-
 ?
trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylonitrile + H2O
-
 Rhodococcus sp. trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylic acid + NH3
-
 ?

Synonyms

Synonyms Comment Organism
nitrilase AtNIT1
-
 Arabidopsis thaliana
nitrilase AtNIT1
-
 Rhodococcus sp.