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Literature summary for 3.5.5.1 extracted from

  • Vejvoda, V.; Kaplan, O.; Klozova, J.; Masak, J.; Cejkova, A.; Jirku, V.; Stloukal, R.; Martinkova, L.
    Mild hydrolysis of nitriles by Fusarium solani strain O1 (2006), Folia Microbiol. (Praha), 51, 251-256.
    View publication on PubMed
Search for more literature for 3.5.5.1

Organism

Organism UniProt Comment Textmining
Fusarium solani
-
-
-
Fusarium solani O1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Fusarium solani

Source Tissue

Source Tissue Comment Organism Textmining
mycelium high levels of aromatic nitrilase is induced after transfer of the mycelium from a rich medium into a medium with 20 mM picolinonitrile Fusarium solani
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzonitrile + 2 H2O
-
 Fusarium solani benzoic acid + NH3
-
 ?
benzonitrile + 2 H2O
-
 Fusarium solani O1 benzoic acid + NH3
-
 ?
isonicotinonitrile + H2O
-
 Fusarium solani isonicotinic acid + NH3
-
 ?
isonicotinonitrile + H2O
-
 Fusarium solani O1 isonicotinic acid + NH3
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 soluble and immobilized enzyme Fusarium solani

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 30°C: about 50% of maximal activity, 55°C: about 50% of maximal activitysoluble enzyme Fusarium solani
30 55 35°C: about 40% of maximal activity, 55°C: about 40% of maximal activity, immobilized enzyme Fusarium solani

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 half tome: 4.5 h Fusarium solani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 soluble enzyme Fusarium solani
9 10 immobilized enzyme Fusarium solani

pH Range

pH Minimum pH Maximum Comment Organism
5 10 pH 5.0: about 40% of maximal activity, pH 10.0: about 75% of maximal activity Fusarium solani
6 11.5 pH 6.0: about 55% of maximal activity, immobilized enzyme, pH 11.5: about 50% of maximal activity Fusarium solani