Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhodococcus rhodochrous |
Protein Variants | Comment | Organism |
---|---|---|
C165A | no activity | Rhodococcus rhodochrous |
C165S | no activity | Rhodococcus rhodochrous |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | complete loss of activity due to modification of Cys165 | Rhodococcus rhodochrous |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzonitrile + 2 H2O | Rhodococcus rhodochrous | - |
benzoic acid + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus rhodochrous | - |
- |
- |
Purification (Comment) | Organism |
---|---|
highly purified, recombinant enzyme | Rhodococcus rhodochrous |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.04 | - |
- |
Rhodococcus rhodochrous |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzonitrile + 2 H2O | - |
Rhodococcus rhodochrous | benzoic acid + NH3 | - |
? |