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Literature summary for 3.5.4.B9 extracted from

  • Rausch, J.W.; Chelico, L.; Goodman, M.F.; Le Grice, S.F.
    Dissecting APOBEC3G substrate specificity by nucleoside analog interference (2009), J. Biol. Chem., 284, 7047-7058.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D316R/D317R the mutations increase affinity for substrate and deamination specificity Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
x * 46000, the enzyme is active in monomeric, dimeric, and larger oligomeric states Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytosine in single-stranded viral DNA + H2O Homo sapiens the enzyme targets 5'-CCCA-3' sequences with 5'-AAACCCAAA-3' recognized most efficiently uracil in single-stranded viral DNA + NH3
-
?
additional information Homo sapiens the enzyme does not effectively bind substrates shorter than 10 nucleotides. Substrates containing 5'-methyldeoxycytidine 2'-deoxy-5-aza-5,6-dihydrocytidine, 2'-deoxy-4-ethylcytidine and 2'-deoxyzebularine at position -1 are deaminated by the enzyme with 62%, 25%, 19%, and 9% efficiency, respectively, Substrates containing N3-methyl cytidine or isocytidine at position -1 are not appreciably deaminated by the enzyme ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose resin column chromatography and DEAE FF column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AAAGAGAAAGAGAAACCCAAAGAGGAAAGGTGAGGAGAA-3' + H2O the enzyme targets 5'-CCCA-3' sequences with 5'-AAACCCAAA-3' recognized most efficiently Homo sapiens 5'-AAAGAGAAAGAGAAACCUAAAGAGGAAAGGTGAGGAGAA-3' + NH3
-
?
cytosine in single-stranded viral DNA + H2O the enzyme targets 5'-CCCA-3' sequences with 5'-AAACCCAAA-3' recognized most efficiently Homo sapiens uracil in single-stranded viral DNA + NH3
-
?
additional information the enzyme does not effectively bind substrates shorter than 10 nucleotides. Substrates containing 5'-methyldeoxycytidine 2'-deoxy-5-aza-5,6-dihydrocytidine, 2'-deoxy-4-ethylcytidine and 2'-deoxyzebularine at position -1 are deaminated by the enzyme with 62%, 25%, 19%, and 9% efficiency, respectively, Substrates containing N3-methyl cytidine or isocytidine at position -1 are not appreciably deaminated by the enzyme Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
monomer or dimer x * 46000, the enzyme is active in monomeric, dimeric, and larger oligomeric states Homo sapiens

Synonyms

Synonyms Comment Organism
A3G
-
Homo sapiens
APOBEC3G
-
Homo sapiens
apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G
-
Homo sapiens