BRENDA - Enzyme Database
show all sequences of 3.5.4.B9

Dissecting APOBEC3G substrate specificity by nucleoside analog interference

Rausch, J.W.; Chelico, L.; Goodman, M.F.; Le Grice, S.F.; J. Biol. Chem. 284, 7047-7058 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Sf9 insect cells
Homo sapiens
Engineering
Protein Variants
Commentary
Organism
D316R/D317R
the mutations increase affinity for substrate and deamination specificity
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
contains zinc
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
-
x * 46000, the enzyme is active in monomeric, dimeric, and larger oligomeric states
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
cytosine in single-stranded viral DNA + H2O
Homo sapiens
the enzyme targets 5'-CCCA-3' sequences with 5'-AAACCCAAA-3' recognized most efficiently
uracil in single-stranded viral DNA + NH3
-
-
?
additional information
Homo sapiens
the enzyme does not effectively bind substrates shorter than 10 nucleotides. Substrates containing 5'-methyldeoxycytidine 2'-deoxy-5-aza-5,6-dihydrocytidine, 2'-deoxy-4-ethylcytidine and 2'-deoxyzebularine at position -1 are deaminated by the enzyme with 62%, 25%, 19%, and 9% efficiency, respectively, Substrates containing N3-methyl cytidine or isocytidine at position -1 are not appreciably deaminated by the enzyme
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
glutathione-Sepharose resin column chromatography and DEAE FF column chromatography
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5'-AAAGAGAAAGAGAAACCCAAAGAGGAAAGGTGAGGAGAA-3' + H2O
the enzyme targets 5'-CCCA-3' sequences with 5'-AAACCCAAA-3' recognized most efficiently
722665
Homo sapiens
5'-AAAGAGAAAGAGAAACCUAAAGAGGAAAGGTGAGGAGAA-3' + NH3
-
-
-
?
cytosine in single-stranded viral DNA + H2O
the enzyme targets 5'-CCCA-3' sequences with 5'-AAACCCAAA-3' recognized most efficiently
722665
Homo sapiens
uracil in single-stranded viral DNA + NH3
-
-
-
?
additional information
the enzyme does not effectively bind substrates shorter than 10 nucleotides. Substrates containing 5'-methyldeoxycytidine 2'-deoxy-5-aza-5,6-dihydrocytidine, 2'-deoxy-4-ethylcytidine and 2'-deoxyzebularine at position -1 are deaminated by the enzyme with 62%, 25%, 19%, and 9% efficiency, respectively, Substrates containing N3-methyl cytidine or isocytidine at position -1 are not appreciably deaminated by the enzyme
722665
Homo sapiens
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer or dimer
x * 46000, the enzyme is active in monomeric, dimeric, and larger oligomeric states
Homo sapiens
Synonyms
Synonyms
Commentary
Organism
A3G
-
Homo sapiens
APOBEC3G
-
Homo sapiens
apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G
-
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Sf9 insect cells
Homo sapiens
Engineering (protein specific)
Protein Variants
Commentary
Organism
D316R/D317R
the mutations increase affinity for substrate and deamination specificity
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
contains zinc
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
-
x * 46000, the enzyme is active in monomeric, dimeric, and larger oligomeric states
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
cytosine in single-stranded viral DNA + H2O
Homo sapiens
the enzyme targets 5'-CCCA-3' sequences with 5'-AAACCCAAA-3' recognized most efficiently
uracil in single-stranded viral DNA + NH3
-
-
?
additional information
Homo sapiens
the enzyme does not effectively bind substrates shorter than 10 nucleotides. Substrates containing 5'-methyldeoxycytidine 2'-deoxy-5-aza-5,6-dihydrocytidine, 2'-deoxy-4-ethylcytidine and 2'-deoxyzebularine at position -1 are deaminated by the enzyme with 62%, 25%, 19%, and 9% efficiency, respectively, Substrates containing N3-methyl cytidine or isocytidine at position -1 are not appreciably deaminated by the enzyme
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
glutathione-Sepharose resin column chromatography and DEAE FF column chromatography
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5'-AAAGAGAAAGAGAAACCCAAAGAGGAAAGGTGAGGAGAA-3' + H2O
the enzyme targets 5'-CCCA-3' sequences with 5'-AAACCCAAA-3' recognized most efficiently
722665
Homo sapiens
5'-AAAGAGAAAGAGAAACCUAAAGAGGAAAGGTGAGGAGAA-3' + NH3
-
-
-
?
cytosine in single-stranded viral DNA + H2O
the enzyme targets 5'-CCCA-3' sequences with 5'-AAACCCAAA-3' recognized most efficiently
722665
Homo sapiens
uracil in single-stranded viral DNA + NH3
-
-
-
?
additional information
the enzyme does not effectively bind substrates shorter than 10 nucleotides. Substrates containing 5'-methyldeoxycytidine 2'-deoxy-5-aza-5,6-dihydrocytidine, 2'-deoxy-4-ethylcytidine and 2'-deoxyzebularine at position -1 are deaminated by the enzyme with 62%, 25%, 19%, and 9% efficiency, respectively, Substrates containing N3-methyl cytidine or isocytidine at position -1 are not appreciably deaminated by the enzyme
722665
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer or dimer
x * 46000, the enzyme is active in monomeric, dimeric, and larger oligomeric states
Homo sapiens
Other publictions for EC 3.5.4.B9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734319
Lu
Crystal structure of DNA cytid ...
Homo sapiens
J. Biol. Chem.
290
4010-4021
2015
-
-
1
1
12
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13
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1
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2
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1
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1
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2
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13
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1
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12
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13
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1
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1
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1
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13
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12
12
735015
Zhu
Host APOBEC3G protein inhibits ...
Homo sapiens
PLoS ONE
10
e0121608
2015
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-
-
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1
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1
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1
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1
1
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754123
Lu
Crystal structure of DNA cyti ...
Homo sapiens
J. Biol. Chem.
290
4010-4021
2015
-
-
1
1
13
-
-
26
-
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2
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3
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2
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19
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1
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1
13
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26
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3
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19
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23
23
721999
Jaszczur
AID and Apobec3G haphazard dea ...
Homo sapiens
Cell. Mol. Life Sci.
70
3089-3108
2013
-
-
-
-
1
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1
1
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3
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1
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3
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1
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2
1
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-
-
-
-
-
1
1
-
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-
734795
Holtz
APOBEC3G cytosine deamination ...
Homo sapiens
Nucleic Acids Res.
41
6139-6148
2013
-
-
-
-
-
-
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-
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1
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1
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2
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718465
Li
First-in-class small molecule ...
Homo sapiens
ACS Chem. Biol.
7
506-517
2012
-
-
-
1
-
-
34
-
-
-
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1
-
5
-
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1
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1
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1
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3
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34
-
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1
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34
34
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1
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1
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1
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1
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720014
Senavirathne
Single-stranded DNA scanning a ...
Homo sapiens
J. Biol. Chem.
287
15826-15835
2012
1
-
-
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2
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1
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2
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1
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2
1
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1
1
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721668
Shlyakhtenko
Nanoscale structure and dynami ...
Homo sapiens
Biochemistry
51
6432-6440
2012
-
-
-
-
-
-
-
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1
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1
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1
1
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1
1
-
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722064
Olson
Small-molecule APOBEC3G DNA cy ...
Homo sapiens
ChemMedChem
8
112-117
2012
-
-
-
-
2
-
5
-
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1
-
1
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1
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1
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1
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2
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4
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2
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4
5
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1
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1
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1
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1
-
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-
-
-
-
-
-
1
1
-
-
-
723071
Sadler
APOBEC3G contributes to HIV-1 ...
Homo sapiens
J. Virol.
84
7396-7404
2012
-
-
1
-
-
-
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-
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1
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1
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1
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1
1
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723754
Monajemi
Emerging complexities of APOBE ...
Homo sapiens
Retrovirology
9
35
2012
-
-
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1
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1
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1
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2
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1
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2
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1
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1
1
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719945
Demorest
Phosphorylation directly regul ...
Homo sapiens, Mus musculus
J. Biol. Chem.
286
26568-26575
2011
-
-
2
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7
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2
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5
-
2
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7
2
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2
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2
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7
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2
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2
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721103
Li
Functional analysis of the two ...
Homo sapiens
Virology
414
130-136
2011
-
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1
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1
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1
1
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-
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722698
Feng
Intensity of deoxycytidine dea ...
Homo sapiens
J. Biol. Chem.
286
11415-11426
2011
-
-
1
-
2
-
-
-
-
-
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2
-
2
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1
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2
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2
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1
1
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712428
Chelico
Structural model for deoxycyti ...
Homo sapiens
J. Biol. Chem.
285
16195-16205
2010
-
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5
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1
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1
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2
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1
1
5
1
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5
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1
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719342
Carpenter
Determinants of sequence-speci ...
Homo sapiens
DNA Repair
9
579-587
2010
-
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1
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1
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1
1
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722114
Furukawa
Structure, interaction and rea ...
Homo sapiens
EMBO J.
28
440-451
2009
-
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1
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10
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1
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1
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1
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10
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1
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1
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722665
Rausch
Dissecting APOBEC3G substrate ...
Homo sapiens
J. Biol. Chem.
284
7047-7058
2009
-
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1
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1
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1
1
2
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1
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1
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3
1
3
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1
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1
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1
1
2
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1
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3
1
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723261
Holden
Crystal structure of the anti- ...
Homo sapiens
Nature
456
121-124
2008
-
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1
1
10
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1
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1
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1
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1
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1
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1
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1
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1
10
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1
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1
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1
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1
-
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722104
Pham
DNA deaminases AID and APOBEC3 ...
Homo sapiens
DNA Repair
6
689-692
2007
-
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1
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1
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1
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1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722103
Coker
The nuclear DNA deaminase AID ...
Homo sapiens
DNA Repair
6
235-243
2006
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
1
-
-
-
-
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1
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1
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1
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1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
723256
Chelico
APOBEC3G DNA deaminase acts pr ...
Homo sapiens
Nat. Struct. Mol. Biol.
13
392-399
2006
-
-
1
-
-
-
-
-
1
-
1
2
-
2
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
723285
Suspene
APOBEC3G is a single-stranded ...
Homo sapiens
Nucleic Acids Res.
32
2421-2429
2004
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
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3
-
1
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1
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-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
723260
Zhang
The cytidine deaminase CEM15 i ...
Homo sapiens
Nature
424
94-98
2003
-
-
1
-
4
-
-
-
-
1
-
1
-
3
-
-
1
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
1
-
1
-
-
-
1
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1
-
-
1
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1
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