Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3/RIL) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F202A | the mutation causes a decrease of the enzyme activity | Homo sapiens |
F241K | the mutation causes a decrease of the enzyme activity | Homo sapiens |
L235A | the mutation causes a decrease of the enzyme activity | Homo sapiens |
L235K | the mutation causes a decrease of the enzyme activity | Homo sapiens |
L242A | the mutation causes a decrease of the enzyme activity | Homo sapiens |
L242K | the mutation causes a decrease of the enzyme activity | Homo sapiens |
T203A | the mutation causes a decrease of the enzyme activity | Homo sapiens |
V233A | the mutation causes a decrease of the enzyme activity | Homo sapiens |
V233K | the mutation causes a decrease of the enzyme activity | Homo sapiens |
W232A | the mutation causes a decrease of the enzyme activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9HC16 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni Sepharose resin column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytosine in single-stranded viral DNA + H2O | minus strand deamination occurs preferentially at a CCCA sequence. The third cytidine of the d(CCCA) segment is deaminated at an early stage and that then the second one is deaminated at a late stage, so the deamination is carried out in a strict 3'-5' order | Homo sapiens | uracil in single-stranded viral DNA + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APOBEC3G | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme exhibits anti-human immunodeficiency virus-1 (HIV-1) activity by deaminating cytidines of the minus strand of HIV-1. Virus infectivity factor of HIV-1 counteracts the anti-HIV-1 activity of the enzyme | Homo sapiens |