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Literature summary for 3.5.4.9 extracted from
- Methylene tetrahydrofolate dehydrogenase/cyclohydrolase and the synthesis of 10-CHO-THF are essential in Leishmania major (2009), Mol. Microbiol., 71, 1386-1401.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| hexa-His tagged DHCH1 is expressed in Escherichia coli, production of a genetic knock-out | Leishmania major |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Leishmania major | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methenyltetrahydrofolate + H2O | Leishmania major | - |
10-formyltetrahydrofolate | - |
r |  |
| additional information | Leishmania major | dehydrogenase and cyclohydrogenase activities are contained in the single bifunctional protein DHCH1 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania major | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant DHCH1 protein is purified by metal-affinity chromatography | Leishmania major |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
cyclohydrolase activity is monitored by following the consumption of 5,10-methylenetetrahydrofolate yielding 10-formyltetrahydrofolate | Leishmania major |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methenyltetrahydrofolate + H2O | - |
Leishmania major | 10-formyltetrahydrofolate | - |
r | |
| additional information | dehydrogenase and cyclohydrogenase activities are contained in the single bifunctional protein DHCH1 | Leishmania major | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydrogenase | - |
Leishmania major |
| DHCH1 | - |
Leishmania major |
| methylene tetrahydrofolate dehydrogenase/cyclohydrolase | - |
Leishmania major |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | - |
assay at | Leishmania major |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
assay at | Leishmania major |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Leishmania major | |
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Release 2023.1 (January 2023)
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