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Literature summary for 3.5.4.9 extracted from
- A 5,10-methylenetetrahydrofolate dehydrogenase: 5,10-methenyltetrahydrofolate cyclohydrolase protein from Pisum sativum (1995), Phytochemistry, 39, 1309-1317.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dihydrofolate | - |
Pisum sativum |  |
| folic acid | - |
Pisum sativum | |
| NADP+ | - |
Pisum sativum | |
| Pyridine nucleotide | - |
Pisum sativum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Pisum sativum | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
SDS-PAGE | Escherichia coli |
| 38500 | - |
SDS-PAGE | Pisum sativum |
| 40000 | - |
Western blot analysis, cross-reactivity with purified pea enzyme | Vicia faba |
| 40000 | - |
Western blot analysis, cross-reactivity with purified pea enzyme | Triticum aestivum |
| 40000 | - |
Western blot analysis, cross-reactivity with purified pea enzyme | Hordeum vulgare |
| 40000 | - |
Western blot analysis, cross-reactivity with purified pea enzyme | Zea mays |
| 40000 | - |
Western blot analysis, cross-reactivity with purified pea enzyme | Neurospora sp. |
| 58000 | - |
gel filtration | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methenyltetrahydrofolate + H2O | Vicia faba | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Escherichia coli | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Triticum aestivum | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Hordeum vulgare | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Spinacia oleracea | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Pisum sativum | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Zea mays | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Moorella thermoacetica | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Neurospora sp. | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Gottschalkia acidurici | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Escherichia coli BE | - |
10-formyltetrahydrofolate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
bifunctional enzyme | - |
| Escherichia coli BE | - |
bifunctional enzyme | - |
| Gottschalkia acidurici | - |
- |
- |
| Hordeum vulgare | - |
barley | - |
| Moorella thermoacetica | - |
bifunctional enzyme | - |
| Neurospora sp. | - |
trifunctional enzyme | - |
| Pisum sativum | - |
bifunctional enzyme | - |
| Spinacia oleracea | - |
spinach | - |
| Triticum aestivum | - |
wheat | - |
| Vicia faba | - |
bean | - |
| Zea mays | - |
corn | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Pisum sativum | - |
| leaf | - |
Vicia faba | - |
| leaf | - |
Triticum aestivum | - |
| leaf | - |
Hordeum vulgare | - |
| leaf | - |
Spinacia oleracea | - |
| leaf | - |
Pisum sativum | - |
| leaf | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.5 | - |
- |
Pisum sativum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 25% glycerol, 20% cyclohydrolase activity remains after 1 month | Pisum sativum |
| 4°C, 25% glycerol, 60% cyclohydrolase activity remains after 1 month | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methenyltetrahydrofolate + H2O | - |
Vicia faba | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Escherichia coli | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Triticum aestivum | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Hordeum vulgare | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Spinacia oleracea | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Pisum sativum | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Zea mays | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Moorella thermoacetica | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Neurospora sp. | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Gottschalkia acidurici | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Escherichia coli BE | 10-formyltetrahydrofolate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 31000, SDS-PAGE | Escherichia coli |
| monomer | 1 * 58000 | Pisum sativum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5,10-CH+-H4PteGlu cyclohydrolase | - |
Pisum sativum |
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