General Stability | Organism |
---|---|
unstable in absence of reducing agents, completely inactive without cysteine, cysteine, sodium hydrosulfite, glutathione, sodium thioglycolate and ascorbic acid stabilizes enzyme activity | Clostridium cylindrosporum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Amino-1,2,4-triazole | - |
Clostridium cylindrosporum | |
4-Hydroxy-1,2,3-triazole | less effective inhibitor | Clostridium cylindrosporum | |
Co2+ | addition of 0.002 M reduces activity by 50% or more | Clostridium cylindrosporum | |
EDTA | - |
Clostridium cylindrosporum | |
histamine | less effective inhibitor | Clostridium cylindrosporum | |
Mg2+ | addition of 0.002 M reduces activity by 50% or more | Clostridium cylindrosporum | |
Mn2+ | addition of 0.002 M reduces activity by 50% or more | Clostridium cylindrosporum | |
Zn2+ | addition of 0.002 M reduces activity by 50% or more | Clostridium cylindrosporum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
4-aminoimidazole | - |
Clostridium cylindrosporum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | restores activity of EDTA treated enzyme to 83% of the original activity | Clostridium cylindrosporum | |
Fe2+ | divalent cation required for activity | Clostridium cylindrosporum | |
Mn2+ | restores activity of EDTA treated enzyme to 60% of the original activity | Clostridium cylindrosporum | |
Ni2+ | restores activity of EDTA treated enzyme to 23% of the original activity | Clostridium cylindrosporum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminoimidazole + H2O | Clostridium cylindrosporum | - |
formiminoglycine + NH3 + unidentified product | alkali-labile derivative of glycine, can be hydrolyzed to yield glycine | ? | |
4-aminoimidazole + H2O | Clostridium cylindrosporum HC1 | - |
formiminoglycine + NH3 + unidentified product | alkali-labile derivative of glycine, can be hydrolyzed to yield glycine | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium cylindrosporum | - |
- |
- |
Clostridium cylindrosporum HC1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Clostridium cylindrosporum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
140 | - |
- |
Clostridium cylindrosporum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminoimidazole + H2O | - |
Clostridium cylindrosporum | formiminoglycine + NH3 + unidentified product | - |
? | |
4-aminoimidazole + H2O | - |
Clostridium cylindrosporum | formiminoglycine + NH3 + unidentified product | alkali-labile derivative of glycine, can be hydrolyzed to yield glycine | ? | |
4-aminoimidazole + H2O | - |
Clostridium cylindrosporum HC1 | formiminoglycine + NH3 + unidentified product | - |
? | |
4-aminoimidazole + H2O | - |
Clostridium cylindrosporum HC1 | formiminoglycine + NH3 + unidentified product | alkali-labile derivative of glycine, can be hydrolyzed to yield glycine | ? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
in triethanolamine hydrochloride | Clostridium cylindrosporum |