Literature summary for 3.5.4.6 extracted from

Dieni, C.A.; Storey, K.B.
Regulation of 5'-adenosine monophosphate deaminase in the freeze tolerant wood frog, Rana sylvatica (2008), BMC Biochem., 9, 12.

Search for more literature for 3.5.4.6

Activating Compound

Activating Compound	Comment	Organism	Structure
ADP	muscle AMPD is activated by Mg ADP-	Lithobates sylvaticus
ATP	muscle AMPD is activated by Mg ATP2-	Lithobates sylvaticus
additional information	the percentage of bound AMPD activity increases from 20 to 35% with the transition to the frozen state	Lithobates sylvaticus

Inhibitors

Inhibitors	Comment	Organism	Structure
GTP	very strong inhibitor	Lithobates sylvaticus
IMP	uniquely inhibits only the bound (phosphorylated) enzyme from muscle of frozen frogs	Lithobates sylvaticus
K+	very weak inhibitor of free AMPD, but shows stronger effect on bound AMPD	Lithobates sylvaticus
Na+	very weak inhibitor of free AMPD, but shows stronger effect on bound AMPD	Lithobates sylvaticus
NH3	-	Lithobates sylvaticus

Organism

Organism	UniProt	Comment	Textmining
Lithobates sylvaticus	-	also known as Lithobates sylvaticus	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	the reversible phosphorylation adjusts enzyme function for an optimal role in controlling cellular adenylate levels in ischemic frozen muscle	Lithobates sylvaticus

Purification (Commentary)

Purification (Comment)	Organism
Sephadex G50 gel filtration	Lithobates sylvaticus

Source Tissue

Source Tissue	Comment	Organism	Textmining
skeletal muscle	-	Lithobates sylvaticus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
AMP + H2O	-	Lithobates sylvaticus	IMP + NH3	-	?

Synonyms

Synonyms	Comment	Organism
5'-adenosine monophosphate deaminase	-	Lithobates sylvaticus
AMP deaminase	-	Lithobates sylvaticus
AmpD	-	Lithobates sylvaticus

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.08	-	free AMPD from frozen skeletal muscle, in 50 mM MOPS buffer (pH 7.2), at 25°C	Lithobates sylvaticus	GTP
0.1	-	myosin-bound AMPD from frozen skeletal muscle, in 50 mM MOPS buffer (pH 7.2), at 25°C	Lithobates sylvaticus	GTP
0.23	-	myosin-bound AMPD from frozen skeletal muscle, in 50 mM MOPS buffer (pH 7.2), at 25°C	Lithobates sylvaticus	IMP
133	-	myosin-bound AMPD from frozen skeletal muscle, in 50 mM MOPS buffer (pH 7.2), at 25°C	Lithobates sylvaticus	Na+
162	-	myosin-bound AMPD from frozen skeletal muscle, in 50 mM MOPS buffer (pH 7.2), at 25°C	Lithobates sylvaticus	K+
254	-	free AMPD from frozen skeletal muscle, in 50 mM MOPS buffer (pH 7.2), at 25°C	Lithobates sylvaticus	NH3
702	-	free AMPD from frozen skeletal muscle, in 50 mM MOPS buffer (pH 7.2), at 25°C	Lithobates sylvaticus	K+
824	-	free AMPD from frozen skeletal muscle, in 50 mM MOPS buffer (pH 7.2), at 25°C	Lithobates sylvaticus	Na+

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Release 2023.1 (January 2023)