Literature summary for 3.5.4.5 extracted from

Giovannetti, E.; Laan, A.C.; Vasile, E.; Tibaldi, C.; Nannizzi, S.; Ricciardi, S.; Falcone, A.; Danesi, R.; Peters, G.J.
Correlation between cytidine deaminase genotype and gemcitabine deamination in blood samples (2008), Nucleosides Nucleotides Nucleic Acids, 27, 720-725.

Search for more literature for 3.5.4.5

Application

Application	Comment	Organism
diagnostics	evaluation of CDA genotype may be helpful in screening patients before treatment, in order to identify subjects with slower CDA-mediated gemcitabine metabolism	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cytidine + H2O	Homo sapiens	-	uridine + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
blood	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.536	-	activity in non-small cell lung cancer patients with CDA Lys27Lys protein, in 50 mM beta-mercaptoethanol, 0.1 M Tris-HCl, pH 8.0, substrate 0.25 mM gemcitabine	Homo sapiens
0.7254	-	activity in non-small cell lung cancer patients carrying the Lys27Gln polymorphism and the Gln27Gln polymorphism, in 50 mM beta-mercaptoethanol, 0.1 M Tris-HCl, pH 8.0, substrate 0.25 mM gemcitabine	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cytidine + H2O	-	Homo sapiens	uridine + NH3	-	?
gemcitabine + H2O	CDA is the major enzyme involved in gemcitabine inactivation, a cytotoxic drug commonly used in the treatment of pancreas and non-small cell lung cancer	Homo sapiens	2'-deoxy-2',2'-difluorouridine	-	?

Synonyms

Synonyms	Comment	Organism
CDA	-	Homo sapiens
cytidine deaminase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)