Literature summary for 3.5.4.5 extracted from

  • Lemaire, M.; Momparler, L.F.; Raynal, N.J.; Bernstein, M.L.; Momparler, R.L.
    Inhibition of cytidine deaminase by zebularine enhances the antineoplastic action of 5-aza-2-deoxycytidine (2009), Cancer Chemother. Pharmacol., 63, 411-416.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
functional expression of the human enzyme in murine fibroblast cell lines 3T3 NIH and 3T3-CD3-V5 cells, and in the murine lymphocytic leukemia cell line L1210 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
zebularine inhibits the enzyme, but also DNA methylation, competitive versus 5-aza-2'-deoxycytidine, zebularine affects the antineoplastic action of 5-AZA-CdR on 3T3 cells and V5 cells transduced with CR deaminase gene, overview Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0477
-
5-aza-2'-deoxycytidine pH 7.4, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytidine + H2O Homo sapiens cytidine deaminase is a key enzyme in the catabolism of cytosine nucleoside analogues, since their deamination results in a loss of their pharmacological activity uridine + NH3
-
?
5-aza-2'-deoxycytidine + H2O Homo sapiens 5-AZA-CdR shows an antineoplastic action on 3T3 cells and V5 cells transduced with CR deaminase gene, pharmakokinetic analysis in mice, overview 5-aza-2'-deoxyuridine + NH3
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme partially from 3T3 NIH and 3T3-CD3-V5 cells by ammonium sulfate fractionation Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytidine + H2O
-
Homo sapiens uridine + NH3
-
?
cytidine + H2O cytidine deaminase is a key enzyme in the catabolism of cytosine nucleoside analogues, since their deamination results in a loss of their pharmacological activity Homo sapiens uridine + NH3
-
?
5-aza-2'-deoxycytidine + H2O
-
Homo sapiens 5-aza-2'-deoxyuridine + NH3
-
?
5-aza-2'-deoxycytidine + H2O 5-AZA-CdR shows an antineoplastic action on 3T3 cells and V5 cells transduced with CR deaminase gene, pharmakokinetic analysis in mice, overview Homo sapiens 5-aza-2'-deoxyuridine + NH3
-
?

Synonyms

Synonyms Comment Organism
CR deaminase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00095
-
zebularine pH 7.4, 37°C Homo sapiens