Cloned (Comment) | Organism |
---|---|
functional expression of the human enzyme in murine fibroblast cell lines 3T3 NIH and 3T3-CD3-V5 cells, and in the murine lymphocytic leukemia cell line L1210 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
zebularine | inhibits the enzyme, but also DNA methylation, competitive versus 5-aza-2'-deoxycytidine, zebularine affects the antineoplastic action of 5-AZA-CdR on 3T3 cells and V5 cells transduced with CR deaminase gene, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0477 | - |
5-aza-2'-deoxycytidine | pH 7.4, 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-aza-2'-deoxycytidine + H2O | Homo sapiens | 5-AZA-CdR shows an antineoplastic action on 3T3 cells and V5 cells transduced with CR deaminase gene, pharmakokinetic analysis in mice, overview | 5-aza-2'-deoxyuridine + NH3 | - |
? | |
cytidine + H2O | Homo sapiens | cytidine deaminase is a key enzyme in the catabolism of cytosine nucleoside analogues, since their deamination results in a loss of their pharmacological activity | uridine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme partially from 3T3 NIH and 3T3-CD3-V5 cells by ammonium sulfate fractionation | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-aza-2'-deoxycytidine + H2O | - |
Homo sapiens | 5-aza-2'-deoxyuridine + NH3 | - |
? | |
5-aza-2'-deoxycytidine + H2O | 5-AZA-CdR shows an antineoplastic action on 3T3 cells and V5 cells transduced with CR deaminase gene, pharmakokinetic analysis in mice, overview | Homo sapiens | 5-aza-2'-deoxyuridine + NH3 | - |
? | |
cytidine + H2O | - |
Homo sapiens | uridine + NH3 | - |
? | |
cytidine + H2O | cytidine deaminase is a key enzyme in the catabolism of cytosine nucleoside analogues, since their deamination results in a loss of their pharmacological activity | Homo sapiens | uridine + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CR deaminase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00095 | - |
zebularine | pH 7.4, 37°C | Homo sapiens |