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Literature summary for 3.5.4.5 extracted from
- Recombinant human cytidine deaminase: Expression, purification, and characterization (1996), Protein Expr. Purif., 8, 247-253.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA, PCR, vector pTrc99A, cloned and expressed in Escherichia coli DH5alpha | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,4,5,6-Tetrahydrouridine | recombinant CDA | Homo sapiens |  |
| 3-deazauridine | recombinant enzyme | Homo sapiens | |
| 5-fluoro-2'-deoxyuridine | recombinant enzyme | Homo sapiens | |
| 5-{(1E)-3-[(5-carboxypentyl)amino]-3-oxoprop-1-en-1-yl}-5,6-dihydrocytidine | recombinant enzyme | Homo sapiens | |
| CMP | recombinant CDA | Homo sapiens | |
| thymine riboside | recombinant CDA | Homo sapiens | |
| uridine | - |
Escherichia coli | |
| uridine | recombinant enzyme | Homo sapiens | |
| UTP | recombinant CDA | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.039 | - |
cytidine | recombinant CDA | Homo sapiens | |
| 0.039 | - |
deoxycytidine | recombinant CDA | Homo sapiens | |
| 0.04 | - |
5-methylcytidine | recombinant CDA | Homo sapiens | |
| 0.1 | - |
5-iododeoxycytidine | recombinant CDA | Homo sapiens | |
| 0.11 | - |
cytosine arabinoside | recombinant CDA | Homo sapiens | |
| 0.28 | - |
5-azacytidine | recombinant CDA | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
4 * 15000, SDS-PAGE, purified enzyme, homotetramer | Homo sapiens |
| 16000 | - |
estimated from cDNA open reading frame coding for human cytidine deaminase | Homo sapiens |
| 16170 | - |
deduced from cDNA nucleotide sequence | Homo sapiens |
| 32000 | - |
2 * 32000, homodimer, SDS-PAGE | Escherichia coli |
| 57000 | - |
gel filtration | Escherichia coli |
| 57000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cytidine + H2O | Bacillus subtilis | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Escherichia coli | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Homo sapiens | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Escherichia coli DH5-alpha | - |
uridine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Escherichia coli | - |
strain DH5alpha | - |
| Escherichia coli DH5-alpha | - |
strain DH5alpha | - |
| Homo sapiens | - |
human | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| granulocyte | - |
Homo sapiens | - |
| leukocyte | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| lymphocyte | - |
Homo sapiens | - |
| placenta | - |
Homo sapiens | - |
| spleen | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 105 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20 mM Tris-HCl, pH 7.5, 1 mM DDT, 1 mM EDTA, 20% glycerol, enzyme retains full activity for at least 1 month, 80% of activity lost in absence of glycerol | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-beta-D-arabinofuranosylcytosine + H2O | - |
Escherichia coli | ? | - |
? | |
| 1-beta-D-arabinofuranosylcytosine + H2O | - |
Homo sapiens | ? | - |
? | |
| 1-beta-D-arabinofuranosylcytosine + H2O | - |
Escherichia coli DH5-alpha | ? | - |
? | |
| 5-azacytidine + H2O | - |
Homo sapiens | 5-azauridine + NH3 | - |
? | |
| 5-azadeoxycytidine + H2O | - |
Homo sapiens | 5-azadeoxyuridine + NH3 | - |
? | |
| 5-methylcytidine + H2O | - |
Homo sapiens | 5-methyluridine + NH3 | - |
? | |
| cytidine + H2O | - |
Bacillus subtilis | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Escherichia coli | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Homo sapiens | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Escherichia coli DH5-alpha | uridine + NH3 | - |
? | |
| deoxycytidine + H2O | - |
Escherichia coli | deoxyuridine + NH3 | - |
? | |
| deoxycytidine + H2O | - |
Homo sapiens | deoxyuridine + NH3 | - |
? | |
| deoxycytidine + H2O | - |
Escherichia coli DH5-alpha | deoxyuridine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 32000, homodimer, SDS-PAGE | Escherichia coli |
| tetramer | - |
Bacillus subtilis |
| tetramer | 4 * 16000, amino acid sequence | Homo sapiens |
| tetramer | 4 * 15000, SDS-PAGE, purified enzyme, homotetramer | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 10.5 | deoxycytidine as substrate, beyond these values irreversible inactivation occurs | Homo sapiens |
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