BRENDA - Enzyme Database
show all sequences of 3.5.4.44

A blueprint of ectoine metabolism from the genome of the industrial producer Halomonas elongata DSM 2581 T

Schwibbert, K.; Marin-Sanguino, A.; Bagyan, I.; Heidrich, G.; Lentzen, G.; Seitz, H.; Rampp, M.; Schuster, S.C.; Klenk, H.P.; Pfeiffer, F.; Oesterhelt, D.; Kunte, H.J.; Environ. Microbiol. 13, 1973-1994 (2011)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Halomonas elongata
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44900
-
-
Halomonas elongata
Organism
Organism
UniProt
Commentary
Textmining
Halomonas elongata
E1V7W1
-
-
Halomonas elongata DSM 2581
E1V7W1
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ectoine + H2O
-
740379
Halomonas elongata
N2-acetyl-L-2,4-diaminobutanoate
-
-
-
?
ectoine + H2O
-
740379
Halomonas elongata DSM 2581
N2-acetyl-L-2,4-diaminobutanoate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 44900, calculated
Halomonas elongata
Synonyms
Synonyms
Commentary
Organism
doeA
-
Halomonas elongata
Helo_3665
-
Halomonas elongata
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Halomonas elongata
calculated
-
5
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Halomonas elongata
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44900
-
-
Halomonas elongata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ectoine + H2O
-
740379
Halomonas elongata
N2-acetyl-L-2,4-diaminobutanoate
-
-
-
?
ectoine + H2O
-
740379
Halomonas elongata DSM 2581
N2-acetyl-L-2,4-diaminobutanoate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 44900, calculated
Halomonas elongata
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Halomonas elongata
calculated
-
5
General Information
General Information
Commentary
Organism
physiological function
the degradation of ectoine proceeds via hydrolysis of ectoine by DoeA to Nalpha-acetyl-L-2,4-diaminobutanoate, followed by deacetylation to diaminobutanoate by DoeB and a transaminase reaction by DoeD leading to aspartate-semialdehyde. Deletion of doeA results in a mutant that cannot utilize ectoine as carbon and nitrogen source
Halomonas elongata
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the degradation of ectoine proceeds via hydrolysis of ectoine by DoeA to Nalpha-acetyl-L-2,4-diaminobutanoate, followed by deacetylation to diaminobutanoate by DoeB and a transaminase reaction by DoeD leading to aspartate-semialdehyde. Deletion of doeA results in a mutant that cannot utilize ectoine as carbon and nitrogen source
Halomonas elongata
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740379
Schwibbert
A blueprint of ectoine metabol ...
Halomonas elongata, Halomonas elongata DSM 2581
Environ. Microbiol.
13
1973-1994
2011
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-