Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.4.42 extracted from

  • Shapir, N.; Osborne, J.P.; Johnson, G.; Sadowsky, M.J.; Wackett, L.P.
    Purification, substrate range, and metal center of AtzC: the N-isopropylammelide aminohydrolase involved in bacterial atrazine metabolism (2002), J. Bacteriol., 184, 5376-5384.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 5 mM, 30% residual activity, Mn2+, Co2+ or Ni2+ restores Pseudomonas sp.
2-amino-4-hydroxy-1,3,5-s-triazine i.e, 5-azacytosine, competitive Pseudomonas sp.
8-Hydroxyquinoline-5-sulfonic acid 5 mM, 30% residual activity, Mn2+, Ni2+ or Co2+ restores Pseudomonas sp.
ammeline competitive Pseudomonas sp.
EDTA 5 mM, about 10% loss of activity Pseudomonas sp.
N-ethylammeline
-
Pseudomonas sp.
N-hydroxyethylammeline competitve Pseudomonas sp.
N-isopropylammeline
-
Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.155
-
N-dimethylammelide pH 7.6, 25°C Pseudomonas sp.
0.299
-
N-tert-butylammelide pH 7.6, 25°C Pseudomonas sp.
0.308
-
N-ethylammelide pH 7.6, 25°C Pseudomonas sp.
0.406
-
N-isopropylammelide pH 7.6, 25°C Pseudomonas sp.
0.58
-
N-cyclopropylammelide pH 7.6, 25°C Pseudomonas sp.
0.817
-
N-methylammelide pH 7.6, 25°C Pseudomonas sp.
1.32
-
ammelide pH 7.6, 25°C Pseudomonas sp.
3.86
-
N-hydroxyethylammelide pH 7.6, 25°C Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ completely restores activity of metal-depleted enzyme Pseudomonas sp.
Mn2+ completely restores activity of metal-depleted enzyme Pseudomonas sp.
Ni2+ completely restores activity of metal-depleted enzyme Pseudomonas sp.
Zn2+ 0.5 mol per mol of subunit Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
4 * 44000, SDS-PAGE, 4 * 44900, deduced from gene sequence Pseudomonas sp.
44900
-
4 * 44000, SDS-PAGE, 4 * 44900, deduced from gene sequence Pseudomonas sp.
174000
-
gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pseudomonas sp.

Renatured (Commentary)

Renatured (Comment) Organism
activity of metal-depleted enzyme may be restored by Zn2+, Fe2+, Mn2+, Co2+, Ni2+ Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.2
-
25°C, pH 7.6 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ammelide + H2O
-
Pseudomonas sp. cyanuric acid + NH3
-
?
N-cyclopropylammelide + H2O
-
Pseudomonas sp. cyanuric acid + cyclopropylamine
-
?
N-dimethylammelide + H2O
-
Pseudomonas sp. cyanuric acid + dimethylamine
-
?
N-ethylammelide + H2O
-
Pseudomonas sp. cyanuric acid + ethylamine
-
?
N-hydroxyethylammelide + H2O
-
Pseudomonas sp. cyanuric acid + 2-aminoethanol
-
?
N-isopropylammelide + H2O
-
Pseudomonas sp. cyanuric acid + isopropylamine
-
?
N-methylammelide + H2O
-
Pseudomonas sp. cyanuric acid + methylamine
-
?
N-tert-butylammelide + H2O
-
Pseudomonas sp. cyanuric acid + tert-butylamine
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 44000, SDS-PAGE, 4 * 44900, deduced from gene sequence Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.015
-
2-amino-4-hydroxy-1,3,5-s-triazine pH 7.6, 25°C Pseudomonas sp.
0.129
-
N-hydroxyethylammeline pH 7.6, 25°C Pseudomonas sp.
0.687
-
ammeline pH 7.6, 25°C Pseudomonas sp.