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Literature summary for 3.5.4.41 extracted from

  • Miller, D.; O'Brien, K.; Xu, H.; White, R.H.
    Identification of a 5'-deoxyadenosine deaminase in Methanocaldococcus jannaschii and its possible role in recycling the radical S-adenosylmethionine enzyme reaction product 5'-deoxyadenosine (2013), J. Bacteriol., 196, 1064-1072.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
EDTA stimulates Methanocaldococcus jannaschii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
C294S the wild-type and the C294S mutant strains are stable after heating for 10 min at 60°C Methanocaldococcus jannaschii
E150R less thermostable than wild-type enzyme, stable for 10 min at 50°C, showing a significant loss of activity at 60°C. kcat/Km for 5'-deoxyadenosine is 93fold lower than the kcat/Km for the wild-type enzyme Methanocaldococcus jannaschii
Y136R less thermostable than wild-type enzyme, stable for 10 min at 50°C, showing a significant loss of activity at 60°C. kcat/Km for 5'-deoxyadenosine is 535fold lower than the kcat/Km for the wild-type enzyme Methanocaldococcus jannaschii
Y136R/E150R less thermostable than wild-type enzyme. kcat/Km for 5'-deoxyadenosine is 290000fold lower than the kcat/Km for the wild-type enzyme Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
5'-deoxyadenosine pH and temperature not specified in the publication, wild-type enzyme Methanocaldococcus jannaschii
0.021
-
5'-deoxyadenosine pH and temperature not specified in the publication, mutant enzyme E150R Methanocaldococcus jannaschii
0.032
-
5'-deoxyadenosine pH and temperature not specified in the publication, mutant enzyme Y136R Methanocaldococcus jannaschii
0.05
-
5'-deoxyadenosine pH and temperature not specified in the publication, mutant enzyme Y136R/E150R Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the activity found in the enzymes expressed with added Zn(II) and added Fe(II) are 2fold higher than when expressed with Ni(II) or without additional metal Methanocaldococcus jannaschii
additional information metal-dependent enzyme. The metal can not be removed from the enzyme nor can the nature of the catalytic metal be established. Dialysis of the wild-type enzyme for 3 days against a dipicolinic acid containing buffer fails to show any lowering enzyme activity, again indicating that chelation is not able to remove the bound metal in the active site. The catalytic metal is not removed by EDTA Methanocaldococcus jannaschii
Ni2+ the activity found in the enzymes expressed with added Zn(II) and added Fe(II) are 2fold higher than when expressed with Ni(II) or without additional metal Methanocaldococcus jannaschii
Zn2+ the activity found in the enzymes expressed with added Zn(II) and added Fe(II) are 2fold higher than when expressed with Ni(II) or without additional metal Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57500
-
4 * 57500 Methanocaldococcus jannaschii
230000
-
gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-deoxyadenosine + H2O Methanocaldococcus jannaschii the enzyme is involved in the recycling of 5'-deoxyadenosine, whereupon the 5'-deoxyribose moiety of 5'-deoxyinosine is further metabolized to deoxyhexoses used for the biosynthesis of aromatic amino acids in methanogens 5'-deoxyinosine + NH3
-
?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58936
-
-
Methanocaldococcus jannaschii DSM 2661 Q58936
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-deoxyadenosine + H2O the enzyme is involved in the recycling of 5'-deoxyadenosine, whereupon the 5'-deoxyribose moiety of 5'-deoxyinosine is further metabolized to deoxyhexoses used for the biosynthesis of aromatic amino acids in methanogens Methanocaldococcus jannaschii 5'-deoxyinosine + NH3
-
?
5'-deoxyadenosine + H2O catalytic efficiency of the enzyme for 5'-deoxyadenosine is 1000fold higher than for S-methyl-5'-thioadenosine, and 10000fold higher than with S-adenosyl-L-homocysteine or adenosine Methanocaldococcus jannaschii 5'-deoxyinosine + NH3
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 57500 Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
DadD
-
Methanocaldococcus jannaschii
MJ1541 locus name Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
10 min, mutant enzyme Y136R/E150R loses 65% of its activity, mutant enzyme Y136R loses 20% of its activity, mutant enzyme E150R loses% of its activity Methanocaldococcus jannaschii
60
-
10 min, wild-type enzyme loses 10% of its activity, mutant enzyme Y136R loses 60% of its activity, mutant enzyme E150R loses 75% of its activity Methanocaldococcus jannaschii
70
-
10 min, mutant enzyme loses 66% of its activity Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.12
-
5'-deoxyadenosine pH and temperature not specified in the publication, mutant enzyme Y136R/E150R Methanocaldococcus jannaschii
21
-
5'-deoxyadenosine pH and temperature not specified in the publication, mutant enzyme E150R Methanocaldococcus jannaschii
330
-
5'-deoxyadenosine pH and temperature not specified in the publication, mutant enzyme Y136R Methanocaldococcus jannaschii
120000
-
5'-deoxyadenosine pH and temperature not specified in the publication, wild-type enzyme Methanocaldococcus jannaschii

General Information

General Information Comment Organism
metabolism the enzyme is involved in the recycling of 5'-deoxyadenosine, whereupon the 5'-deoxyribose moiety of 5'-deoxyinosine is further metabolized to deoxyhexoses used for the biosynthesis of aromatic amino acids in methanogens Methanocaldococcus jannaschii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
31
-
5'-deoxyadenosine pH and temperature not specified in the publication, mutant enzyme Y136R/E150R Methanocaldococcus jannaschii
17000
-
5'-deoxyadenosine pH and temperature not specified in the publication, mutant enzyme Y136R Methanocaldococcus jannaschii
98000
-
5'-deoxyadenosine pH and temperature not specified in the publication, mutant enzyme E150R Methanocaldococcus jannaschii
9100000
-
5'-deoxyadenosine pH and temperature not specified in the publication, wild-type enzyme Methanocaldococcus jannaschii