Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Deinococcus radiodurans |
expressed in Escherichia coli BL21(DE3) cells | Streptomyces avermitilis |
expressed in Escherichia coli BL21(DE3) cells | Nitratiruptor sp. SB155-2 |
expressed in Escherichia coli Rosetta2 (DE3) cells | Acidothermus cellulolyticus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 30% (w/v) PEG MME500, 0.1 M Bis-Tris, pH 6.5, and 50 mM calcium chloride | Deinococcus radiodurans |
Protein Variants | Comment | Organism |
---|---|---|
R87A | the mutant shows reduced activity towards 6-amino-6-deoxyfutalosine and adenosine compared to the wild type enzyme | Streptomyces avermitilis |
R87M | the mutant shows reduced activity towards 6-amino-6-deoxyfutalosine and increased activity towards adenosine compared to the wild type enzyme | Streptomyces avermitilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
6-amino-6-deoxyfutalosine | wild type enzyme, at pH 7.5 and at 30°C | Nitratiruptor sp. SB155-2 | |
0.0018 | - |
6-amino-6-deoxyfutalosine | wild type enzyme, at pH 7.5 and at 30°C | Deinococcus radiodurans | |
0.0034 | - |
6-amino-6-deoxyfutalosine | mutant enzyme R87A, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
0.0048 | - |
6-amino-6-deoxyfutalosine | wild type enzyme, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
0.006 | - |
6-amino-6-deoxyfutalosine | wild type enzyme, at pH 7.5 and at 30°C | Acidothermus cellulolyticus | |
0.008 | - |
6-amino-6-deoxyfutalosine | mutant enzyme R87M, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
0.0083 | - |
adenosine | mutant enzyme R87A, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
0.01 | - |
adenosine | mutant enzyme R87M, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
0.037 | - |
2'-deoxyadenosine | wild type enzyme, at pH 7.5 and at 30°C | Deinococcus radiodurans | |
0.042 | - |
5'-deoxyadenosine | wild type enzyme, at pH 7.5 and at 30°C | Nitratiruptor sp. SB155-2 | |
0.061 | - |
1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-beta-D-ribofuranuronamide | wild type enzyme, at pH 7.5 and at 30°C | Acidothermus cellulolyticus | |
0.094 | - |
5'-methylthioadenosine | wild type enzyme, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
0.1 | 1 | 5'-methylthioadenosine | wild type enzyme, at pH 7.5 and at 30°C | Nitratiruptor sp. SB155-2 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains Zn2+ | Deinococcus radiodurans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-amino-6-deoxyfutalosine + H2O | Acidothermus cellulolyticus | - |
futalosine + NH3 | - |
? | |
6-amino-6-deoxyfutalosine + H2O | Deinococcus radiodurans | - |
futalosine + NH3 | - |
? | |
6-amino-6-deoxyfutalosine + H2O | Streptomyces avermitilis | - |
futalosine + NH3 | - |
? | |
6-amino-6-deoxyfutalosine + H2O | Nitratiruptor sp. SB155-2 | - |
futalosine + NH3 | - |
? | |
6-amino-6-deoxyfutalosine + H2O | Acidothermus cellulolyticus 11B | - |
futalosine + NH3 | - |
? | |
6-amino-6-deoxyfutalosine + H2O | Streptomyces avermitilis MA-4680 | - |
futalosine + NH3 | - |
? | |
6-amino-6-deoxyfutalosine + H2O | Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 | - |
futalosine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidothermus cellulolyticus | A0LRH8 | - |
- |
Acidothermus cellulolyticus 11B | A0LRH8 | - |
- |
Deinococcus radiodurans | Q9RW45 | - |
- |
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 | Q9RW45 | - |
- |
Nitratiruptor sp. SB155-2 | A6Q234 | - |
- |
Streptomyces avermitilis | Q82K09 | - |
- |
Streptomyces avermitilis MA-4680 | Q82K09 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Acidothermus cellulolyticus |
HisTrap column chromatography and Superdex 200 gel filtration | Deinococcus radiodurans |
HisTrap column chromatography and Superdex 200 gel filtration | Streptomyces avermitilis |
Strep-Tactin column column chromatography, HisTrap column chromatography, and Superdex 200 gel filtration | Nitratiruptor sp. SB155-2 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-beta-D-ribofuranuronamide + H2O | - |
Acidothermus cellulolyticus | ? | - |
? | |
1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-beta-D-ribofuranuronamide + H2O | - |
Deinococcus radiodurans | ? | - |
? | |
1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-beta-D-ribofuranuronamide + H2O | - |
Streptomyces avermitilis | ? | - |
? | |
1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-beta-D-ribofuranuronamide + H2O | - |
Nitratiruptor sp. SB155-2 | ? | - |
? | |
1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-beta-D-ribofuranuronamide + H2O | - |
Acidothermus cellulolyticus 11B | ? | - |
? | |
1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-beta-D-ribofuranuronamide + H2O | - |
Streptomyces avermitilis MA-4680 | ? | - |
? | |
1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-beta-D-ribofuranuronamide + H2O | - |
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 | ? | - |
? | |
2'-deoxyadenosine + H2O | - |
Acidothermus cellulolyticus | ? | - |
? | |
2'-deoxyadenosine + H2O | - |
Deinococcus radiodurans | ? | - |
? | |
2'-deoxyadenosine + H2O | - |
Streptomyces avermitilis | ? | - |
? | |
2'-deoxyadenosine + H2O | - |
Nitratiruptor sp. SB155-2 | ? | - |
? | |
3'-deoxyadenosine + H2O | - |
Acidothermus cellulolyticus | ? | - |
? | |
3'-deoxyadenosine + H2O | - |
Deinococcus radiodurans | ? | - |
? | |
3'-deoxyadenosine + H2O | - |
Streptomyces avermitilis | ? | - |
? | |
3'-deoxyadenosine + H2O | - |
Nitratiruptor sp. SB155-2 | ? | - |
? | |
5'-deoxyadenosine + H2O | - |
Acidothermus cellulolyticus | ? | - |
? | |
5'-deoxyadenosine + H2O | - |
Deinococcus radiodurans | ? | - |
? | |
5'-deoxyadenosine + H2O | - |
Streptomyces avermitilis | ? | - |
? | |
5'-deoxyadenosine + H2O | - |
Nitratiruptor sp. SB155-2 | ? | - |
? | |
5'-methylthioadenosine + H2O | - |
Acidothermus cellulolyticus | ? | - |
? | |
5'-methylthioadenosine + H2O | - |
Deinococcus radiodurans | ? | - |
? | |
5'-methylthioadenosine + H2O | - |
Streptomyces avermitilis | ? | - |
? | |
5'-methylthioadenosine + H2O | - |
Nitratiruptor sp. SB155-2 | ? | - |
? | |
5'-methylthioadenosine + H2O | - |
Acidothermus cellulolyticus 11B | ? | - |
? | |
5'-methylthioadenosine + H2O | - |
Streptomyces avermitilis MA-4680 | ? | - |
? | |
5'-methylthioadenosine + H2O | - |
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 | ? | - |
? | |
6-amino-6-deoxyfutalosine + H2O | - |
Acidothermus cellulolyticus | futalosine + NH3 | - |
? | |
6-amino-6-deoxyfutalosine + H2O | - |
Deinococcus radiodurans | futalosine + NH3 | - |
? | |
6-amino-6-deoxyfutalosine + H2O | - |
Streptomyces avermitilis | futalosine + NH3 | - |
? | |
6-amino-6-deoxyfutalosine + H2O | - |
Nitratiruptor sp. SB155-2 | futalosine + NH3 | - |
? | |
6-amino-6-deoxyfutalosine + H2O | - |
Acidothermus cellulolyticus 11B | futalosine + NH3 | - |
? | |
6-amino-6-deoxyfutalosine + H2O | - |
Streptomyces avermitilis MA-4680 | futalosine + NH3 | - |
? | |
6-amino-6-deoxyfutalosine + H2O | - |
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 | futalosine + NH3 | - |
? | |
adenosine + H2O | - |
Acidothermus cellulolyticus | ? | - |
? | |
adenosine + H2O | - |
Deinococcus radiodurans | ? | - |
? | |
adenosine + H2O | - |
Streptomyces avermitilis | ? | - |
? | |
adenosine + H2O | - |
Nitratiruptor sp. SB155-2 | ? | - |
? | |
AMP + H2O | - |
Acidothermus cellulolyticus | ? | - |
? | |
AMP + H2O | - |
Deinococcus radiodurans | ? | - |
? | |
AMP + H2O | - |
Streptomyces avermitilis | ? | - |
? | |
AMP + H2O | - |
Nitratiruptor sp. SB155-2 | ? | - |
? | |
AMP + H2O | - |
Acidothermus cellulolyticus 11B | ? | - |
? | |
AMP + H2O | - |
Streptomyces avermitilis MA-4680 | ? | - |
? | |
AMP + H2O | - |
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 | ? | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Acidothermus cellulolyticus | ? | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Deinococcus radiodurans | ? | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Streptomyces avermitilis | ? | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Nitratiruptor sp. SB155-2 | ? | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Acidothermus cellulolyticus 11B | ? | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Streptomyces avermitilis MA-4680 | ? | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Acel0264 | - |
Acidothermus cellulolyticus |
Dr0824 | - |
Deinococcus radiodurans |
Nis0429 | - |
Nitratiruptor sp. SB155-2 |
Sav2595 | - |
Streptomyces avermitilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-beta-D-ribofuranuronamide | wild type enzyme, at pH 7.5 and at 30°C | Acidothermus cellulolyticus | |
0.017 | - |
5'-deoxyadenosine | wild type enzyme, at pH 7.5 and at 30°C | Nitratiruptor sp. SB155-2 | |
0.094 | - |
6-amino-6-deoxyfutalosine | mutant enzyme R87A, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
0.106 | - |
adenosine | mutant enzyme R87A, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
0.31 | - |
5'-methylthioadenosine | wild type enzyme, at pH 7.5 and at 30°C | Nitratiruptor sp. SB155-2 | |
0.4 | - |
5'-methylthioadenosine | wild type enzyme, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
0.41 | - |
adenosine | mutant enzyme R87M, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
0.42 | - |
6-amino-6-deoxyfutalosine | mutant enzyme R87M, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
0.72 | - |
2'-deoxyadenosine | wild type enzyme, at pH 7.5 and at 30°C | Deinococcus radiodurans | |
1.2 | - |
6-amino-6-deoxyfutalosine | wild type enzyme, at pH 7.5 and at 30°C | Nitratiruptor sp. SB155-2 | |
3.8 | - |
6-amino-6-deoxyfutalosine | wild type enzyme, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
6.8 | - |
6-amino-6-deoxyfutalosine | wild type enzyme, at pH 7.5 and at 30°C | Acidothermus cellulolyticus | |
8.6 | - |
6-amino-6-deoxyfutalosine | wild type enzyme, at pH 7.5 and at 30°C | Deinococcus radiodurans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
AMP | wild type enzyme, at pH 7.5 and at 30°C | Nitratiruptor sp. SB155-2 | |
0.005 | - |
2'-deoxyadenosine | wild type enzyme, at pH 7.5 and at 30°C | Nitratiruptor sp. SB155-2 | |
0.006 | - |
3'-deoxyadenosine | wild type enzyme, at pH 7.5 and at 30°C | Nitratiruptor sp. SB155-2 | |
0.011 | - |
adenosine | wild type enzyme, at pH 7.5 and at 30°C | Nitratiruptor sp. SB155-2 | |
0.02 | - |
adenosine | wild type enzyme, at pH 7.5 and at 30°C | Acidothermus cellulolyticus | |
0.021 | - |
AMP | wild type enzyme, at pH 7.5 and at 30°C | Deinococcus radiodurans | |
0.024 | - |
S-adenosyl-L-homocysteine | wild type enzyme, at pH 7.5 and at 30°C | Acidothermus cellulolyticus | |
0.024 | - |
3'-deoxyadenosine | wild type enzyme, at pH 7.5 and at 30°C | Deinococcus radiodurans | |
0.028 | - |
5'-methylthioadenosine | wild type enzyme, at pH 7.5 and at 30°C | Acidothermus cellulolyticus | |
0.032 | - |
AMP | wild type enzyme, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
0.034 | - |
1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-beta-D-ribofuranuronamide | wild type enzyme, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
0.044 | - |
3'-deoxyadenosine | wild type enzyme, at pH 7.5 and at 30°C | Acidothermus cellulolyticus | |
0.045 | - |
AMP | wild type enzyme, at pH 7.5 and at 30°C | Acidothermus cellulolyticus | |
0.047 | - |
5'-deoxyadenosine | wild type enzyme, at pH 7.5 and at 30°C | Acidothermus cellulolyticus | |
0.051 | - |
2'-deoxyadenosine | wild type enzyme, at pH 7.5 and at 30°C | Acidothermus cellulolyticus | |
0.079 | - |
S-adenosyl-L-homocysteine | wild type enzyme, at pH 7.5 and at 30°C | Nitratiruptor sp. SB155-2 | |
0.11 | - |
1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-beta-D-ribofuranuronamide | wild type enzyme, at pH 7.5 and at 30°C | Acidothermus cellulolyticus | |
0.11 | - |
1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-beta-D-ribofuranuronamide | wild type enzyme, at pH 7.5 and at 30°C | Nitratiruptor sp. SB155-2 | |
0.24 | - |
3'-deoxyadenosine | wild type enzyme, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
0.31 | - |
S-adenosyl-L-homocysteine | wild type enzyme, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
0.41 | - |
5'-deoxyadenosine | wild type enzyme, at pH 7.5 and at 30°C | Nitratiruptor sp. SB155-2 | |
1.1 | - |
2'-deoxyadenosine | wild type enzyme, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
2.8 | - |
5'-methylthioadenosine | wild type enzyme, at pH 7.5 and at 30°C | Nitratiruptor sp. SB155-2 | |
2.9 | - |
5'-deoxyadenosine | wild type enzyme, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
4.3 | - |
5'-methylthioadenosine | wild type enzyme, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
7 | - |
S-adenosyl-L-homocysteine | wild type enzyme, at pH 7.5 and at 30°C | Deinococcus radiodurans | |
10 | - |
1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-beta-D-ribofuranuronamide | wild type enzyme, at pH 7.5 and at 30°C | Deinococcus radiodurans | |
13 | - |
adenosine | mutant enzyme R87A, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
18 | - |
adenosine | wild type enzyme, at pH 7.5 and at 30°C | Deinococcus radiodurans | |
19 | - |
2'-deoxyadenosine | wild type enzyme, at pH 7.5 and at 30°C | Deinococcus radiodurans | |
22 | - |
adenosine | wild type enzyme, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
27 | - |
6-amino-6-deoxyfutalosine | mutant enzyme R87A, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
34 | - |
5'-methylthioadenosine | wild type enzyme, at pH 7.5 and at 30°C | Deinococcus radiodurans | |
41 | - |
adenosine | mutant enzyme R87M, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
53 | - |
6-amino-6-deoxyfutalosine | mutant enzyme R87M, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
74 | - |
5'-deoxyadenosine | wild type enzyme, at pH 7.5 and at 30°C | Deinococcus radiodurans | |
790 | - |
6-amino-6-deoxyfutalosine | wild type enzyme, at pH 7.5 and at 30°C | Streptomyces avermitilis | |
1100 | - |
6-amino-6-deoxyfutalosine | wild type enzyme, at pH 7.5 and at 30°C | Acidothermus cellulolyticus | |
1300 | - |
6-amino-6-deoxyfutalosine | wild type enzyme, at pH 7.5 and at 30°C | Nitratiruptor sp. SB155-2 | |
4800 | - |
6-amino-6-deoxyfutalosine | wild type enzyme, at pH 7.5 and at 30°C | Deinococcus radiodurans |