Literature summary for 3.5.4.4 extracted from

Doshi, K.M.; Loukanina, N.N.; Polowick, P.L.; Holbrook, L.A.
Seed specific expression and analysis of recombinant human adenosine deaminase (hADA) in three host plant species (2016), Transgenic Res., 25, 629-637 .

Cloned(Commentary)

Cloned (Comment)	Organism
the activity of human adenosine deaminase (hADA) expressed in transgenic seeds of three different plant species: pea (Pisum sativum L.), Nicotiana benthamiana L. and tarwi (Lupinus mutabilis Sweet) is compared. All three species are transformed with the same expression vector containing the hADA gene driven by the seed-specific promoter LegA2 with an apoplast targeting pinII signal peptide. During the study, several independent transgenic lines are generated and screened from each plant species and only lines with a single copy of the gene of interest are used for hADA expression analysis. A stable transgenic canola line expressing the ADA protein, under the control of 35S constitutive promoter is used as both as a positive control and for comparative study with the seed specific promoter. The highest activity of the hADA enzyme (U/g seed) is reported in tarwi (4.26 U/g) followed by pea (3.23 U/g) and Nicotiana benthamiana (1.69 U/g)	Homo sapiens

Cloned (Comment)

Organism

the activity of human adenosine deaminase (hADA) expressed in transgenic seeds of three different plant species: pea (Pisum sativum L.), Nicotiana benthamiana L. and tarwi (Lupinus mutabilis Sweet) is compared. All three species are transformed with the same expression vector containing the hADA gene driven by the seed-specific promoter LegA2 with an apoplast targeting pinII signal peptide. During the study, several independent transgenic lines are generated and screened from each plant species and only lines with a single copy of the gene of interest are used for hADA expression analysis. A stable transgenic canola line expressing the ADA protein, under the control of 35S constitutive promoter is used as both as a positive control and for comparative study with the seed specific promoter. The highest activity of the hADA enzyme (U/g seed) is reported in tarwi (4.26 U/g) followed by pea (3.23 U/g) and Nicotiana benthamiana (1.69 U/g)

Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
adenosine + H2O	Homo sapiens	-	inosine + NH3	-	ir

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P00813	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
adenosine + H2O	-	Homo sapiens	inosine + NH3	-	ir

Synonyms

Synonyms	Comment	Organism
HadA	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens