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Literature summary for 3.5.4.4 extracted from

  • Gracia, E.; Cortes, A.; Meana, J.J.; Garcia-Sevilla, J.; Herhsfield, M.S.; Canela, E.I.; Mallol, J.; Lluis, C.; Franco, R.; Casado, V.
    Human adenosine deaminase as an allosteric modulator of human A(1) adenosine receptor: abolishment of negative cooperativity for [H](R)-pia binding to the caudate nucleus (2008), J. Neurochem., 107, 161-170.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli S Phi 3834 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
Homo sapiens
N6-methyladenosine
-
Homo sapiens
purine riboside
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
adenosine 25°C, 50 mM Tris-HCl buffer, pH 7.4, enzyme concentration 1.5 nM Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
SDS-PAGE, calculated value 40754 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenosine + H2O Homo sapiens
-
inosine + NH3
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity, protamine sulfate treatment, Q Sepharose chromatography, purine riboside-Sepharose chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2
-
protamine sulfate supernatant Homo sapiens
52
-
Q Sepharose chromatography Homo sapiens
260
-
purine riboside Sepharose chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine + H2O
-
Homo sapiens inosine + NH3
-
?
adenosine + H2O adenosine deaminase markedly enhances the agonist and antagonist affinity and abolishes the negative cooperativity on agonist binding to human striatial adenosine A1 receptors Homo sapiens inosine + NH3
-
?

Subunits

Subunits Comment Organism
dimer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
ADA
-
Homo sapiens
Adenosine aminohydrolase
-
Homo sapiens
adenosine deaminase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
180
-
adenosine 25°C, 50 mM Tris-HCl buffer, pH 7.4, enzyme concentration 1.5 nM Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.008
-
purine riboside 25°C, 50 mM Tris-HCl buffer, pH 7.4, enzyme concentration 1.5 nM Homo sapiens
0.018
-
N6-methyladenosine 25°C, 50 mM Tris-HCl buffer, pH 7.4, enzyme concentration 1.5 nM Homo sapiens