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Literature summary for 3.5.4.4 extracted from

  • Pospisilova, H.; Sebela, M.; Novak, O.; Frebort, I.
    Hydrolytic cleavage of N6-substituted adenine derivatives by eukaryotic adenine and adenosine deaminases (2008), Biosci. Rep., 28, 335-347.
    View publication on PubMed
Search for more literature for 3.5.4.4

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21 (DE3) Schizosaccharomyces pombe
overexpression in Escherichia coli BL21 (DE3) Saccharomyces cerevisiae
overexpression in Escherichia coli BL21 (DE3) Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 adenine
-
 Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39630
-
 calculated Saccharomyces cerevisiae
39950
-
 calculated Arabidopsis thaliana
41200
-
 calculated Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8LPL7
-
-
Saccharomyces cerevisiae P53909 this enzyme catalyses the reaction of EC 3.5.4.2 and EC 3.5.4.4; strain 23344c
-
Schizosaccharomyces pombe Q9P6I7 this enzyme catalyses the reaction of EC 3.5.4.2 and EC 3.5.4.4; wild-type 972 h
-

Purification (Commentary)

Purification (Comment) Organism
using Ni2+-nitrilotriacetate (Ni2+-NTA) column chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 326 nkat/mg with substrate adenine, reaction of EC 3.5.4.2, adenine deaminase Schizosaccharomyces pombe
0.12
-
 0.033 mM adenosine in 0.2 M phosphate buffer (pH 6.7), 30°C Arabidopsis thaliana

Storage Stability

Storage Stability Organism
activity of purified protein is stable for 1-2 weeks when stored at 4°C at concentrations above 10 mg/ml with the addition of 20% (v/v) glycerol. Prolonged storage in solution is possible at -20°C with 50% (v/v) glycerol and at -80°C with 10-20% (v/v) glycerol or as freeze-dried powder Schizosaccharomyces pombe
activity of purified protein is stable for 1-2 weeks when stored at 4°C at concentrations above 10 mg/ml with the addition of 20% (v/v) glycerol. Prolonged storage in solution is possible at -20°C with 50% (v/v) glycerol and at -80°C with 10-20% (v/v) glycerol or as freeze-dried powder Saccharomyces cerevisiae
activity of purified proteins is stable for 1-2 weeks when stored at 4°C at concentrations above 10 mg/ml with the addition of 20% (v/v) glycerol. Prolonged storage in solution is possible at -20°C with 50% (v/v) glycerol and at -80°C with 10-20% (v/v) glycerol or as freeze-dried powder Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine + H2O
-
 Schizosaccharomyces pombe hypoxanthine + NH3
-
 ?
adenine + H2O
-
 Arabidopsis thaliana ? + NH3
-
 ?
adenosine + H2O 1.4% of the activity of adenine Saccharomyces cerevisiae inosine + NH3
-
 ?
adenosine + H2O 2.0% of the activity of adenine Schizosaccharomyces pombe inosine + NH3
-
 ?
adenosine + H2O 30% of the activity of adenine Arabidopsis thaliana inosine + NH3
-
 ir
adenosine 3'-monophosphate + H2O 56% of the activity of adenine Arabidopsis thaliana inosine 3'-monophosphate + NH3
-
 ?
adenosine 3'-phosphate + H2O 3.3% of the activity of adenine Saccharomyces cerevisiae inosine 3'-phosphate + NH3
-
 ?
adenosine 3'-phosphate + H2O 4.5% of the activity of adenine Schizosaccharomyces pombe inosine 3'-phosphate + NH3
-
 ?
ATP + H2O 3.3% of the activity of adenine Schizosaccharomyces pombe ITP + NH3
-
 ?
ATP + H2O 3.3% of the activity of adenine Saccharomyces cerevisiae ITP + NH3
-
 ?
ATP + H2O 68% of the activity of adenine Arabidopsis thaliana ITP + NH3
-
 ?
additional information does not cleave cytokinins Arabidopsis thaliana ?
-
 ?
N6-isopentenyladenosine + H2O 1.9% of the activity of adenine Schizosaccharomyces pombe ?
-
 ?
N6-isopentenyladenosine + H2O 1.2% of the activity of adenine Saccharomyces cerevisiae inosine + 3-methylbut-3-en-1-amine
-
 ?

Synonyms

Synonyms Comment Organism
ADA
-
 Arabidopsis thaliana
adenosine deaminase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 0.2 M phosphate buffer, pH 6.7 Arabidopsis thaliana
30 37 0.2 M phosphate buffer, pH 7.0 Saccharomyces cerevisiae
33
-
 0.2 M phosphate buffer, pH 6.7 Schizosaccharomyces pombe

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 adenosine
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
 0.2 M phosphate buffer, 30°C Arabidopsis thaliana
6.7
-
 0.2 M phosphate buffer, 33°C Schizosaccharomyces pombe
7
-
 0.2 M phosphate buffer, 30-37°C Saccharomyces cerevisiae