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Literature summary for 3.5.4.4 extracted from
- Adenosine deaminase from camel tick Hyalomma dromedarii: purification and characterization (2006), Exp. Appl. Acarol., 40, 101-111.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
adenosine | pH 7.5, 37°C, ADAII | Hyalomma dromedarii |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Hyalomma dromedarii | |
| Hg2+ | - |
Hyalomma dromedarii | |
| lithium ion | - |
Hyalomma dromedarii | |
| Mg2+ | 91% inhibition | Hyalomma dromedarii | |
| Ni2+ | - |
Hyalomma dromedarii | |
| Zn2+ | - |
Hyalomma dromedarii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
ADAII, gel filtration | Hyalomma dromedarii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine + H2O | Hyalomma dromedarii | adenosine deaminase is involved in purine metabolism and is a key enzyme for the control of the cellular levels of adenosine | inosine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hyalomma dromedarii | - |
three enzyme forms ADAI, ADAII and ADAIII | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzymes ADAI, ADAII and ADAIII, separation by acetone precipitation and anion exchange chromatography, 2fold purification of ADA1 and 5fold of ADAIII, further purification 62.9fold to homogeneity of ADAII by gel filtration | Hyalomma dromedarii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | enzyme activity during embryogenesis, overview | Hyalomma dromedarii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.32 | - |
enzyme at day 9 of embryogenesis | Hyalomma dromedarii |
| 1.99 | - |
enzyme at the first day of embryogenesis | Hyalomma dromedarii |
| 2.16 | - |
enzyme at day 24 of embryogenesis | Hyalomma dromedarii |
| 150.8 | - |
purified ADAII | Hyalomma dromedarii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxyadenosine + H2O | - |
Hyalomma dromedarii | 2'-deoxyinosine + NH3 | - |
? | |
| adenosine + H2O | - |
Hyalomma dromedarii | inosine + NH3 | - |
? | |
| adenosine + H2O | adenosine deaminase is involved in purine metabolism and is a key enzyme for the control of the cellular levels of adenosine | Hyalomma dromedarii | inosine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 x 42000, ADAII, SDS-PAGE | Hyalomma dromedarii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADAI | - |
Hyalomma dromedarii |
| ADAII | - |
Hyalomma dromedarii |
| ADAIII | - |
Hyalomma dromedarii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
ADAII | Hyalomma dromedarii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 80 | temperature profile | Hyalomma dromedarii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 80 | stable up to 40°C, loss of 85% of activity at 70°C, thermal stability profile, overview | Hyalomma dromedarii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
ADAII | Hyalomma dromedarii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | 20% of maximal activity at pH 5.5, 23% at pH 9.0, pH profile | Hyalomma dromedarii |
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