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Literature summary for 3.5.4.4 extracted from
- Purification and characterization of adenosine deaminase from camel skeletal muscle (2002), Int. J. Biochem. Cell Biol., 34, 1608-1618.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 0.029 mM, 50% inhibition | Camelus dromedarius |  |
| Mg2+ | 0.055 mM, 50% inhibition | Camelus dromedarius | |
| Mn2+ | 0.046 mM, 50% inhibition | Camelus dromedarius | |
| purine riboside | - |
Camelus dromedarius | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0147 | - |
adenosine | pH 7.5, 25°C | Camelus dromedarius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
gel filtration | Camelus dromedarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Camelus dromedarius | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Camelus dromedarius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | - |
Camelus dromedarius | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25.87 | - |
- |
Camelus dromedarius |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM Tris-HCl, pH 7.5, 0.1 mM adenosine, 0.01 mg enzyme per ml, stable for 6 months | Camelus dromedarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine + H2O | - |
Camelus dromedarius | inosine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, SDS-PAGE | Camelus dromedarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Camelus dromedarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
half-life: 636 min | Camelus dromedarius |
| 50 | - |
half-life: 61 min | Camelus dromedarius |
| 70 | - |
20 min, complete inactivation | Camelus dromedarius |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18.5 | - |
adenosine | pH 7.5, 25°C | Camelus dromedarius | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.75 | - |
- |
Camelus dromedarius |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
purine riboside | pH 7.5, 25°C | Camelus dromedarius | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Camelus dromedarius | isoelectric focusing | - |
4.9 |
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