Protein Variants | Comment | Organism |
---|---|---|
E58Q/C87A/C90A | the mutant enzyme does not bind to single-stranded DNA at all, though it retains some binding to RNA | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytosine in single-stranded DNA + H2O | Homo sapiens | AID mediates hypermutation by deaminating single stranded DNA. In vivo, single stranded DNA may arise transiently during transcription | uracil in single-stranded DNA + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9GZX7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytosine in single-stranded DNA + H2O | AID mediates hypermutation by deaminating single stranded DNA. In vivo, single stranded DNA may arise transiently during transcription | Homo sapiens | uracil in single-stranded DNA + NH3 | - |
? | |
cytosine in single-stranded DNA + H2O | the purified, tetrameric enzyme can deaminate cytidine residues in single-stranded DNA, but not in RNA. It deaminates only single-stranded DNA. Neither cytidine nor CTP (or dCTP) are good substrates for the enzyme in vitro | Homo sapiens | uracil in single-stranded DNA + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
activation-induced deaminase | - |
Homo sapiens |
AID | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 37 | the enzyme works with varying efficiencies from room temperature to 37°C | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
30 min, protein is active | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.6 | 9 | wide range of pH | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | AID mediates hypermutation by deaminating single stranded DNA. In vivo, single stranded DNA may arise transiently during transcription | Homo sapiens |