Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant strains in which the TAD1 gene is disrupted are viable but lack Tad1p enzyme activity and their tRNAAla is not modified at position A37 | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | high salt concentrations (above 100 mM KCl) inhibit the reaction | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000006 | - |
adenine37 in tRNAAla | 30°C, pH 7,9 | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | the KCl optimum for A37I37 conversion by Tad1p is 25 mM | Saccharomyces cerevisiae | |
Mg2+ | tRNA editing is strictly magnesium dependent with an optimal concentration between 23 mM | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine37 in tRNAAla + H2O | Saccharomyces cerevisiae | - |
hypoxanthine37 in tRNAAla + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P53065 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine37 in tRNAAla + H2O | - |
Saccharomyces cerevisiae | hypoxanthine37 in tRNAAla + NH3 | - |
? | |
adenine37 in tRNAAla + H2O | pre-mRNA and extended dsRNA are not used as substrates by Tad1p in vitro. Tad1p requires both the local conformation of the anticodon loop and a correct folding of the L-shaped tRNAAla substrate to recognize and deaminate A37 | Saccharomyces cerevisiae | hypoxanthine37 in tRNAAla + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Tad1p/scADAT1 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0705 | - |
adenine37 in tRNAAla | 30°C, pH 7,9 | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | the TAD1 gene is not essential in yeast under optimal growth conditions at 30°C | Saccharomyces cerevisiae |