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Literature summary for 3.5.4.31 extracted from

  • Guan, R.; Ho, M.C.; Froehlich, R.F.; Tyler, P.C.; Almo, S.C.; Schramm, V.L.
    Methylthioadenosine deaminase in an alternative quorum sensing pathway in Pseudomonas aeruginosa (2012), Biochemistry, 51, 9094-9103.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.31

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with 5'-methylthiocoformycin, hanging or sitting drop vapor diffusion method, using 1.26 M sodium phosphate (monobasic) and 0.14 M potassium phosphate (dibasic) at a final pH of 5.6 at 18°C Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
2'-deoxycoformycin
-
 Pseudomonas aeruginosa
5'-methylthio-2'-deoxycoformycin
-
 Pseudomonas aeruginosa
5'-methylthiocoformycin
-
 Pseudomonas aeruginosa
5'-phenylthio-2'-deoxycoformycin
-
 Pseudomonas aeruginosa
5'-propylthio-2'-deoxycoformycin
-
 Pseudomonas aeruginosa
coformycin
-
 Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
 S-methyl-5'-thioadenosine at pH 7.4 and 25°C Pseudomonas aeruginosa
0.046
-
 adenosine at pH 7.4 and 25°C Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-methyl-5'-thioadenosine + H2O Pseudomonas aeruginosa 40fold greater catalytic efficiency for S-methyl-5'-thioadenosine than for adenosine S-methyl-5'-thioinosine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HZ64
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine + H2O
-
 Pseudomonas aeruginosa inosine + NH3
-
 ?
additional information the enzyme is inactive with adenine, S-adenosylhomocysteine and guanosine Pseudomonas aeruginosa ?
-
 ?
S-methyl-5'-thioadenosine + H2O 40fold greater catalytic efficiency for S-methyl-5'-thioadenosine than for adenosine Pseudomonas aeruginosa S-methyl-5'-thioinosine + NH3
-
 ?

Synonyms

Synonyms Comment Organism
MTA deaminase
-
 Pseudomonas aeruginosa
MTADA
-
 Pseudomonas aeruginosa
PA3170
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.1
-
 adenosine at pH 7.4 and 25°C Pseudomonas aeruginosa
24.6
-
 S-methyl-5'-thioadenosine at pH 7.4 and 25°C Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000000048
-
 5'-methylthiocoformycin at pH 7.4 and 25°C Pseudomonas aeruginosa
0.000000067
-
 5'-propylthio-2'-deoxycoformycin at pH 7.4 and 25°C Pseudomonas aeruginosa
0.00000008
-
 5'-methylthio-2'-deoxycoformycin at pH 7.4 and 25°C Pseudomonas aeruginosa
0.00000013
-
 5'-phenylthio-2'-deoxycoformycin at pH 7.4 and 25°C Pseudomonas aeruginosa
0.000037
-
 2'-deoxycoformycin at pH 7.4 and 25°C Pseudomonas aeruginosa
0.00009
-
 coformycin at pH 7.4 and 25°C Pseudomonas aeruginosa

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000003
-
 at pH 7.4 and 25°C Pseudomonas aeruginosa 5'-methylthiocoformycin

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
370
-
 adenosine at pH 7.4 and 25°C Pseudomonas aeruginosa
16000
-
 S-methyl-5'-thioadenosine at pH 7.4 and 25°C Pseudomonas aeruginosa