Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | stimulates | Methanocaldococcus jannaschii |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli CodonPlus(DE3)-RIL cells | Methanocaldococcus jannaschii |
expression in Escherichia coli | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
C294S | the wild-type and the C294S mutant strains are stable after heating for 10 min at 60°C | Methanocaldococcus jannaschii |
E150R | less thermostable than wild-type enzyme, stable for only 10 min at 50°C | Methanocaldococcus jannaschii |
E150R | the mutant shows about 4fold increased activity compared to the wild type | Methanocaldococcus jannaschii |
Y136R | less thermostable than wild-type enzyme, stable for only 10 min at 50°C | Methanocaldococcus jannaschii |
Y136R | the mutant shows about 10fold increased activity compared to the wild type | Methanocaldococcus jannaschii |
Y136R/E150R | no activity with adenosine | Methanocaldococcus jannaschii |
Y136R/E150R | the mutant shows strongly reduced activity compared to the wild type | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the presence of 0.02 M dithiothreitol has no effect on the enzymatic activity | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
S-methyl-5'-thioadenosine | pH and temperature not specified in the publication, mutant enzyme Y136R | Methanocaldococcus jannaschii | |
0.022 | - |
S-methyl-5'-thioadenosine | mutant enzyme Y136R, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
0.048 | - |
S-methyl-5'-thioadenosine | pH and temperature not specified in the publication, mutant enzyme E150R | Methanocaldococcus jannaschii | |
0.048 | - |
S-methyl-5'-thioadenosine | mutant enzyme E150R, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
0.072 | - |
adenosine | mutant enzyme Y136R, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
0.073 | - |
S-methyl-5'-thioadenosine | pH and temperature not specified in the publication, mutant enzyme Y136R/E150R | Methanocaldococcus jannaschii | |
0.073 | - |
S-methyl-5'-thioadenosine | mutant enzyme Y136R/E150R, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
0.11 | - |
S-methyl-5'-thioadenosine | pH and temperature not specified in the publication, wild-type enzyme | Methanocaldococcus jannaschii | |
0.11 | - |
S-methyl-5'-thioadenosine | wild type enzyme, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
0.15 | - |
adenosine | mutant enzyme E150R, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
0.15 | - |
adenosine | wild type enzyme, at pH 9.0 and 60°C | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the activity found in the enzymes expressed with added Zn(II) and added Fe(II) are 2fold higher than when expressed with Ni(II) or without additional metal | Methanocaldococcus jannaschii | |
additional information | metal-dependent enzyme. The metal can not be removed from the enzyme nor can the nature of the catalytic metal be established. Dialysis of the wild-type enzyme for 3 days against a dipicolinic acid containing buffer fails to show any lowering enzyme activity, again indicating that chelation is not able to remove the bound metal in the active site. The catalytic metal is not removed by EDTA | Methanocaldococcus jannaschii | |
Ni2+ | the activity found in the enzymes expressed with added Zn(II) and added Fe(II) are 2fold higher than when expressed with Ni(II) or without additional metal | Methanocaldococcus jannaschii | |
Zn2+ | the activity found in the enzymes expressed with added Zn(II) and added Fe(II) are 2fold higher than when expressed with Ni(II) or without additional metal | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57500 | - |
4 * 57500 | Methanocaldococcus jannaschii |
230000 | - |
gel filtration | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-methyl-5'-thioadenosine + H2O | Methanocaldococcus jannaschii | - |
S-methyl-5'-thioinosine + NH3 | - |
? | |
S-methyl-5'-thioadenosine + H2O | Methanocaldococcus jannaschii DSM 2661 | - |
S-methyl-5'-thioinosine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58936 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q58936 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine + H2O | low activity | Methanocaldococcus jannaschii | inosine + NH3 | - |
? | |
adenosine + H2O | low activity | Methanocaldococcus jannaschii DSM 2661 | inosine + NH3 | - |
? | |
S-methyl-5'-thioadenosine + H2O | - |
Methanocaldococcus jannaschii | S-methyl-5'-thioinosine + NH3 | - |
? | |
S-methyl-5'-thioadenosine + H2O | catalytic efficiency of the enzyme for 5'-deoxyadenosine is 1000fold higher than for S-methyl-5'-thioadenosine, and 10000fold higher than with S-adenosyl-L-homocysteine or adenosine | Methanocaldococcus jannaschii | S-methyl-5'-thioinosine + NH3 | - |
? | |
S-methyl-5'-thioadenosine + H2O | - |
Methanocaldococcus jannaschii DSM 2661 | S-methyl-5'-thioinosine + NH3 | - |
? | |
S-methyl-5'-thioadenosine + H2O | catalytic efficiency of the enzyme for 5'-deoxyadenosine is 1000fold higher than for S-methyl-5'-thioadenosine, and 10000fold higher than with S-adenosyl-L-homocysteine or adenosine | Methanocaldococcus jannaschii DSM 2661 | S-methyl-5'-thioinosine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 57500 | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
DadD | - |
Methanocaldococcus jannaschii |
MJ1541 | - |
Methanocaldococcus jannaschii |
MJ1541 | locus name | Methanocaldococcus jannaschii |
MtaD | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, mutant enzyme Y136R/E150R loses 65% of its activity, mutant enzyme Y136R loses 20% of its activity, mutant enzyme E150R loses% of its activity | Methanocaldococcus jannaschii |
60 | - |
10 min, wild-type enzyme loses 10% of its activity, mutant enzyme Y136R loses 60% of its activity, mutant enzyme E150R loses 75% of its activity | Methanocaldococcus jannaschii |
60 | 70 | the enzyme shows 90% of retained activity after 10 min at 60°C and 34% of retained activity after 10 min at 70°C | Methanocaldococcus jannaschii |
70 | - |
10 min, mutant enzyme loses 66% of its activity | Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.39 | - |
S-methyl-5'-thioadenosine | pH and temperature not specified in the publication, mutant enzyme Y136R/E150R | Methanocaldococcus jannaschii | |
1.39 | - |
S-methyl-5'-thioadenosine | mutant enzyme Y136R/E150R, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
110 | - |
adenosine | mutant enzyme E150R, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
110 | - |
adenosine | wild type enzyme, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
140 | - |
adenosine | mutant enzyme Y136R, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
160 | - |
S-methyl-5'-thioadenosine | pH and temperature not specified in the publication, wild-type enzyme | Methanocaldococcus jannaschii | |
160 | - |
S-methyl-5'-thioadenosine | wild type enzyme, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
200 | - |
S-methyl-5'-thioadenosine | pH and temperature not specified in the publication, mutant enzyme E150R | Methanocaldococcus jannaschii | |
200 | - |
S-methyl-5'-thioadenosine | mutant enzyme E150R, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
220 | - |
S-methyl-5'-thioadenosine | pH and temperature not specified in the publication, mutant enzyme Y136R | Methanocaldococcus jannaschii | |
220 | - |
S-methyl-5'-thioadenosine | mutant enzyme Y136R, at pH 9.0 and 60°C | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Methanocaldococcus jannaschii |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the recycling of 5'-deoxyadenosine, whereupon the 5'-deoxyribose moiety of 5'-deoxyinosine is further metabolized to deoxyhexoses used for the biosynthesis of aromatic amino acids in methanogens | Methanocaldococcus jannaschii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19 | - |
S-methyl-5'-thioadenosine | pH and temperature not specified in the publication, mutant enzyme Y136R/E150R | Methanocaldococcus jannaschii | |
19 | - |
S-methyl-5'-thioadenosine | mutant enzyme Y136R/E150R, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
750 | - |
adenosine | mutant enzyme E150R, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
750 | - |
adenosine | wild type enzyme, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
1100 | - |
S-methyl-5'-thioadenosine | pH and temperature not specified in the publication, wild-type enzyme | Methanocaldococcus jannaschii | |
1100 | - |
S-methyl-5'-thioadenosine | wild type enzyme, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
2900 | - |
adenosine | mutant enzyme Y136R, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
4200 | - |
S-methyl-5'-thioadenosine | pH and temperature not specified in the publication, mutant enzyme E150R | Methanocaldococcus jannaschii | |
4200 | - |
S-methyl-5'-thioadenosine | mutant enzyme E150R, at pH 9.0 and 60°C | Methanocaldococcus jannaschii | |
11000 | - |
S-methyl-5'-thioadenosine | pH and temperature not specified in the publication, mutant enzyme Y136R | Methanocaldococcus jannaschii | |
11000 | - |
S-methyl-5'-thioadenosine | mutant enzyme Y136R, at pH 9.0 and 60°C | Methanocaldococcus jannaschii |