Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.4.30 extracted from

  • Huffman, J.L.; Li, H.; White, R.H.; Tainer, J.A.
    Structural basis for recognition and catalysis by the bifunctional dCTP deaminase and dUTPase from Methanococcus jannaschii (2003), J. Mol. Biol., 331, 885-896.
    View publication on PubMed
Search for more literature for 3.5.4.30

Crystallization (Commentary)

Crystallization (Comment) Organism
apo form complexed with dCTP and dUTP, hanging drop vapor diffusion method Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77900
-
 enzyme with addition of six histidines at the N-terminus, gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dCTP + H2O Methanocaldococcus jannaschii first step in conversion of dCTP to dTTP dUMP + diphosphate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q57872
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dCTP + H2O
-
 Methanocaldococcus jannaschii dUMP + diphosphate + NH3
-
 ?
dCTP + H2O first step in conversion of dCTP to dTTP Methanocaldococcus jannaschii dUMP + diphosphate + NH3
-
 ?
additional information enzyme has also dUTP diphosphatase activity, dCTP deamination and dUTP diphosphatase activities occur independently at the same active site Methanocaldococcus jannaschii ?
-
 ?

Subunits

Subunits Comment Organism
hexamer two trimers can assemble into a hexamer by stacking on top of each other Methanocaldococcus jannaschii
trimer two trimers can assemble into a hexamer by stacking on top of each other Methanocaldococcus jannaschii