Application | Comment | Organism |
---|---|---|
food industry | in beer samples treated with adenine deaminase and guanine deaminase, the adenine concentration in beer drops 66-67% and guanine concentration in beer drops from 68.8 microM to a minimal amount | Kluyveromyces lactis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Kluyveromyces lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
guanine | pH 6.0, 30°C | Kluyveromyces lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
10600 | - |
gel filtration | Kluyveromyces lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces lactis | - |
- |
- |
Kluyveromyces lactis MTCC 458 | - |
- |
- |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris, 100 mM NaCl, 10% glycerol and pH 7.0, stable for a month | Kluyveromyces lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-thioguanine + H2O | - |
Kluyveromyces lactis | 6-thioxanthine + NH3 | - |
? | |
8-azaxanthine + H2O | - |
Kluyveromyces lactis | 8-azaxanthine + HCl | - |
? | |
guanine + H2O | preferred substrate | Kluyveromyces lactis | xanthine + NH3 | - |
? | |
guanine + H2O | preferred substrate | Kluyveromyces lactis MTCC 458 | xanthine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | x * 55000, SDS-PAGE | Kluyveromyces lactis |
Synonyms | Comment | Organism |
---|---|---|
GDA | - |
Kluyveromyces lactis |
KLLAOD9492 | - |
Kluyveromyces lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Kluyveromyces lactis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.3 | - |
guanine | pH 6.0, 30°C | Kluyveromyces lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Kluyveromyces lactis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Kluyveromyces lactis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
870 | - |
guanine | pH 6.0, 30°C | Kluyveromyces lactis |