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Literature summary for 3.5.4.3 extracted from

  • Kumar, K.S.; Sitaramayya, A.; Krishnan, P.S.
    Guanine deaminase in rat liver and mouse liver and brain (1972), Biochem. J., 128, 1079-1088.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
GTP positive effector of isoenzyme A, no influence on isoenzyme B Rattus norvegicus

General Stability

General Stability Organism
addition of bovine serum albumin stabilizes the 2 isoenzymes in the cold and isoenzyme A in the frozen state Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ 0.1 mM FeSO4, 82% inhibition Rattus norvegicus
Fe3+ 0.25 mM FeCl3, 82% inhibition Rattus norvegicus
guanidine hydrochloride
-
Rattus norvegicus
HClO4
-
Mus musculus
HClO4
-
Rattus norvegicus
HgCl2 69% inhibition in 5 min Mus musculus
p-hydroxymercuribenzoate isoenzyme B more rapidly inactivated and the inhibition is less effectively reversed by thiol supplements than isoenzyme A Rattus norvegicus
Urea 8 M, both isoenzymes Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
guanine
-
Mus musculus
0.0053
-
guanine isoenzyme A Rattus norvegicus
0.0083
-
8-Azaguanine isoenzyme B Rattus norvegicus
0.018
-
8-Azaguanine isoenzyme A Rattus norvegicus
0.02
-
guanine isoenzyme B Rattus norvegicus
0.066
-
guanine
-
Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.1 mM, 18% activation of isoenzyme A Rattus norvegicus
Cu2+ 0.005 mM 11% activation, isoenzyme A 45% inhibition Rattus norvegicus
Mg2+ 1.0 mM MgSO4, 27% activation of isoenzyme A, 22% of isoenzyme B, 0.5 mM 18% activation of isoenzyme A, 11% activation of isoenzyme B, 1.0 mM MgCl2, 45% activation of isoenzyme A, 33% activation of isoenzyme B, 0.5 mM MgCl2, no effect on isoenzyme A, 11% activation of isoenzyme B Rattus norvegicus
Zn2+ 0.1 mM 11% activation of isoenzyme B, isoenzyme A 27% inhibition Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanine + H2O Mus musculus
-
xanthine + NH3
-
?
guanine + H2O Rattus norvegicus
-
xanthine + NH3
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
mouse
-
Mus musculus
-
albino mice
-
Rattus norvegicus
-
rat
-
Rattus norvegicus
-
albino rats
-

Purification (Commentary)

Purification (Comment) Organism
2 isoenzymes, A and B Rattus norvegicus
liver and brain, 2 isoenzymes, A and B Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Mus musculus
-
brain
-
Rattus norvegicus
-
liver
-
Mus musculus
-
liver 2 isoenzymes Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.42
-
brain enzyme Mus musculus
5.1
-
liver enzyme Mus musculus
9.9
-
liver enzyme, isoenzyme A Rattus norvegicus
33.6
-
isoenzyme B Rattus norvegicus

Storage Stability

Storage Stability Organism
-18°C, isoenzyme A loses about 60% of activity on thawing after 7 days, isoenzyme B is unaltered in activity Rattus norvegicus
0-4°C, activity of isoenzyme A completely lost in 16 days, isoenzyme B loses 30% activity Rattus norvegicus
0°C, retains its activity unaltered during storage for a month Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-azaguanine + H2O
-
Rattus norvegicus 8-azaxanthine + NH3
-
?
guanine + H2O
-
Mus musculus xanthine + NH3
-
?
guanine + H2O
-
Rattus norvegicus xanthine + NH3
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
after incubation for 30 min both isoenzymes A and B retains full activity Rattus norvegicus
60
-
isoenzyme A 33% residual activity, isoenzyme B 60% activity lost Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
8-azaguanine as substrate Rattus norvegicus
7 8
-
Mus musculus
8
-
-
Mus musculus
8
-
-
Rattus norvegicus