Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | positive effector of isoenzyme A, no influence on isoenzyme B | Rattus norvegicus |
General Stability | Organism |
---|---|
addition of bovine serum albumin stabilizes the 2 isoenzymes in the cold and isoenzyme A in the frozen state | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 0.1 mM FeSO4, 82% inhibition | Rattus norvegicus | |
Fe3+ | 0.25 mM FeCl3, 82% inhibition | Rattus norvegicus | |
guanidine hydrochloride | - |
Rattus norvegicus | |
HClO4 | - |
Mus musculus | |
HClO4 | - |
Rattus norvegicus | |
HgCl2 | 69% inhibition in 5 min | Mus musculus | |
p-hydroxymercuribenzoate | isoenzyme B more rapidly inactivated and the inhibition is less effectively reversed by thiol supplements than isoenzyme A | Rattus norvegicus | |
Urea | 8 M, both isoenzymes | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
guanine | - |
Mus musculus | |
0.0053 | - |
guanine | isoenzyme A | Rattus norvegicus | |
0.0083 | - |
8-Azaguanine | isoenzyme B | Rattus norvegicus | |
0.018 | - |
8-Azaguanine | isoenzyme A | Rattus norvegicus | |
0.02 | - |
guanine | isoenzyme B | Rattus norvegicus | |
0.066 | - |
guanine | - |
Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.1 mM, 18% activation of isoenzyme A | Rattus norvegicus | |
Cu2+ | 0.005 mM 11% activation, isoenzyme A 45% inhibition | Rattus norvegicus | |
Mg2+ | 1.0 mM MgSO4, 27% activation of isoenzyme A, 22% of isoenzyme B, 0.5 mM 18% activation of isoenzyme A, 11% activation of isoenzyme B, 1.0 mM MgCl2, 45% activation of isoenzyme A, 33% activation of isoenzyme B, 0.5 mM MgCl2, no effect on isoenzyme A, 11% activation of isoenzyme B | Rattus norvegicus | |
Zn2+ | 0.1 mM 11% activation of isoenzyme B, isoenzyme A 27% inhibition | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + H2O | Mus musculus | - |
xanthine + NH3 | - |
? | |
guanine + H2O | Rattus norvegicus | - |
xanthine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
mouse | - |
Mus musculus | - |
albino mice | - |
Rattus norvegicus | - |
rat | - |
Rattus norvegicus | - |
albino rats | - |
Purification (Comment) | Organism |
---|---|
2 isoenzymes, A and B | Rattus norvegicus |
liver and brain, 2 isoenzymes, A and B | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
brain | - |
Rattus norvegicus | - |
liver | - |
Mus musculus | - |
liver | 2 isoenzymes | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.42 | - |
brain enzyme | Mus musculus |
5.1 | - |
liver enzyme | Mus musculus |
9.9 | - |
liver enzyme, isoenzyme A | Rattus norvegicus |
33.6 | - |
isoenzyme B | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-18°C, isoenzyme A loses about 60% of activity on thawing after 7 days, isoenzyme B is unaltered in activity | Rattus norvegicus |
0-4°C, activity of isoenzyme A completely lost in 16 days, isoenzyme B loses 30% activity | Rattus norvegicus |
0°C, retains its activity unaltered during storage for a month | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-azaguanine + H2O | - |
Rattus norvegicus | 8-azaxanthine + NH3 | - |
? | |
guanine + H2O | - |
Mus musculus | xanthine + NH3 | - |
? | |
guanine + H2O | - |
Rattus norvegicus | xanthine + NH3 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
after incubation for 30 min both isoenzymes A and B retains full activity | Rattus norvegicus |
60 | - |
isoenzyme A 33% residual activity, isoenzyme B 60% activity lost | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
8-azaguanine as substrate | Rattus norvegicus |
7 | 8 | - |
Mus musculus |
8 | - |
- |
Mus musculus |
8 | - |
- |
Rattus norvegicus |