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Literature summary for 3.5.4.28 extracted from
- Purification and characterization of S-adenosylhomocysteine deaminase from streptonigrin-producing Streptomyces flocculus (1989), J. Bacteriol., 171, 6840-6844.
General Stability
| General Stability | Organism |
|---|---|
| unstable to freezing and thawing | Streptomyces albus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 1 mM, 45% loss of activity. 1 mM, 91% loss of activity | Streptomyces albus |  |
| Deoxycoformycin | - |
Streptomyces albus | |
| additional information | not inhibited by metal chelators, iodoacetamide and NEM | Streptomyces albus | |
| PCMB | 0.1 mM, 40% loss of activity | Streptomyces albus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
S-adenosyl-L-homocysteine | - |
Streptomyces albus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56100 | - |
1 * 56100, SDS-PAGE | Streptomyces albus |
| 58300 | - |
sucrose density gradient centrifugation | Streptomyces albus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-homocysteine + H2O | Streptomyces albus | major route of S-adenosylhomocysteine metabolism | adenosine + L-homocysteine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces albus | - |
ATCC 13257 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces albus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.4 | - |
- |
Streptomyces albus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-homocysteine + H2O | - |
Streptomyces albus | adenosine + L-homocysteine | - |
? | |
| S-adenosyl-L-homocysteine + H2O | major route of S-adenosylhomocysteine metabolism | Streptomyces albus | adenosine + L-homocysteine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 56100, SDS-PAGE | Streptomyces albus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Streptomyces albus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
stable at room temperature in presence of 1 mM DTT | Streptomyces albus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26 | - |
S-adenosyl-L-homocysteine | - |
Streptomyces albus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Streptomyces albus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | more than 90% of activity maximum at pH 6.5 and 7.5, rapid drop of activity below pH 6.0 and above 9.0 | Streptomyces albus |
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