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Literature summary for 3.5.4.27 extracted from
- Overproduction and one-step purification of the N5,N10-methenyltetrahydromethanopterin cyclohydrolase (Mch) from the hyperthermophilic Methanopyrus kandleri (1998), Extremophiles, 2, 15-22.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Methanopyrus kandleri |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin | recombinant enzyme | Methanopyrus kandleri |  |
| 0.04 | - |
N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin | - |
Methanopyrus kandleri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
? * 41000, SDS-PAGE | Methanopyrus kandleri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | Methanosarcina barkeri | - |
? | - |
? | |
| 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | Methanopyrus kandleri | - |
? | - |
? | |
| 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | Methanothermobacter thermautotrophicus | enzyme is involved in methanogenesis from CO2 and H2, which represents the energy metabolism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
- |
- |
| Methanopyrus kandleri | - |
- |
- |
| Methanosarcina barkeri | - |
- |
- |
| Methanothermobacter thermautotrophicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanopyrus kandleri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5410 | - |
- |
Methanopyrus kandleri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
Methanosarcina barkeri | N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
| 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
Methanothermobacter thermautotrophicus | N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
| 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
Methanocaldococcus jannaschii | N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
| 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
Methanopyrus kandleri | N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
| 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
Methanosarcina barkeri | ? | - |
? | |
| 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
Methanopyrus kandleri | ? | - |
? | |
| 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | enzyme is involved in methanogenesis from CO2 and H2, which represents the energy metabolism | Methanothermobacter thermautotrophicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | ? * 41000, SDS-PAGE | Methanopyrus kandleri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
pH 8.0, 40 mM K2HPO4, stable up to | Methanopyrus kandleri |
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