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Literature summary for 3.5.4.25 extracted from

  • Singh, M.; Kumar, P.; Yadav, S.; Gautam, R.; Sharma, N.; Karthikeyan, S.
    The crystal structure reveals the molecular mechanism of bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II (Rv1415) from Mycobacterium tuberculosis (2013), Acta Crystallogr. Sect. D, 69, 1633-1644.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M Na HEPES pH 7.5, 15% (w/v) PEG 8000 Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1267
-
GTP full-length bifunctional enzyme ribA2, at pH 8.0 and 37°C Mycobacterium tuberculosis
0.1505
-
GTP GCHII-domain of enzyme ribA2, at pH 8.0 and 37°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mycobacterium tuberculosis
Zn2+ contains zinc Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48163
-
2 * 48163, MALDI-TOF mass spectrometry Mycobacterium tuberculosis
90000
-
gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Mycobacterium tuberculosis
-
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
-
?
GTP + H2O Mycobacterium tuberculosis H37Rv
-
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WHF1
-
-
Mycobacterium tuberculosis H37Rv P9WHF1
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Ni-NTA column chromatography, and Sephacryl S-200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
Mycobacterium tuberculosis formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
-
?
GTP + H2O
-
Mycobacterium tuberculosis H37Rv formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 48163, MALDI-TOF mass spectrometry Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
GCHII
-
Mycobacterium tuberculosis
ribA2 bifunctional enzyme with 3,4-dihydroxy-2-butanone 4-phosphate synthase and GTP cyclohydrolase II activities Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0033
-
GTP full-length bifunctional enzyme ribA2, at pH 8.0 and 37°C Mycobacterium tuberculosis
0.005
-
GTP GCHII-domain of enzyme ribA2, at pH 8.0 and 37°C Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4
-
GTP full-length bifunctional enzyme ribA2, at pH 8.0 and 37°C Mycobacterium tuberculosis
2.1
-
GTP GCHII-domain of enzyme ribA2, at pH 8.0 and 37°C Mycobacterium tuberculosis