Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M Na HEPES pH 7.5, 15% (w/v) PEG 8000 | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1267 | - |
GTP | full-length bifunctional enzyme ribA2, at pH 8.0 and 37°C | Mycobacterium tuberculosis | |
0.1505 | - |
GTP | GCHII-domain of enzyme ribA2, at pH 8.0 and 37°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mycobacterium tuberculosis | |
Zn2+ | contains zinc | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48163 | - |
2 * 48163, MALDI-TOF mass spectrometry | Mycobacterium tuberculosis |
90000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Mycobacterium tuberculosis | - |
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate | - |
? | |
GTP + H2O | Mycobacterium tuberculosis H37Rv | - |
formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WHF1 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WHF1 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Ni-NTA column chromatography, and Sephacryl S-200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Mycobacterium tuberculosis | formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate | - |
? | |
GTP + H2O | - |
Mycobacterium tuberculosis H37Rv | formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 48163, MALDI-TOF mass spectrometry | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
GCHII | - |
Mycobacterium tuberculosis |
ribA2 | bifunctional enzyme with 3,4-dihydroxy-2-butanone 4-phosphate synthase and GTP cyclohydrolase II activities | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
GTP | full-length bifunctional enzyme ribA2, at pH 8.0 and 37°C | Mycobacterium tuberculosis | |
0.005 | - |
GTP | GCHII-domain of enzyme ribA2, at pH 8.0 and 37°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
GTP | full-length bifunctional enzyme ribA2, at pH 8.0 and 37°C | Mycobacterium tuberculosis | |
2.1 | - |
GTP | GCHII-domain of enzyme ribA2, at pH 8.0 and 37°C | Mycobacterium tuberculosis |