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Literature summary for 3.5.4.23 extracted from

  • Li, L.; Wu, J.; Deng, Z.; Zabriskie, T.M.; He, X.
    Streptomyces lividans blasticidin S deaminase and its application in engineering a blasticidin S-producing strain for ease of genetic manipulation (2013), Appl. Environ. Microbiol., 79, 2349-2357.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)/pLysE cells Streptomyces lividans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
blasticidin S + H2O Streptomyces lividans
-
 deaminohydroxyblasticidin S + NH3
-
 ?
blasticidin S + H2O Streptomyces lividans HXY16
-
 deaminohydroxyblasticidin S + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces lividans
-
-
-
Streptomyces lividans HXY16
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography, and gel filtration Streptomyces lividans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-amino-1-(beta-D-glucuronosyl)pyrimidin-2(1H)-one + H2O low activity Streptomyces lividans 1-(beta-D-glucuronosyl)-4-hydroxypyrimidin-2(1H)-one
-
 ?
4-amino-1-(beta-D-glucuronosyl)pyrimidin-2(1H)-one + H2O low activity Streptomyces lividans HXY16 1-(beta-D-glucuronosyl)-4-hydroxypyrimidin-2(1H)-one
-
 ?
blasticidin S + H2O
-
 Streptomyces lividans deaminohydroxyblasticidin S + NH3
-
 ?
blasticidin S + H2O
-
 Streptomyces lividans HXY16 deaminohydroxyblasticidin S + NH3
-
 ?
mildiomycin + H2O low activity Streptomyces lividans ?
-
 ?
mildiomycin + H2O low activity Streptomyces lividans HXY16 ?
-
 ?
additional information the enzyme does not catalyze the deamination of CMP Streptomyces lividans ?
-
 ?
additional information the enzyme does not catalyze the deamination of CMP Streptomyces lividans HXY16 ?
-
 ?

Synonyms

Synonyms Comment Organism
BSD
-
 Streptomyces lividans