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Literature summary for 3.5.4.21 extracted from
- Creatinine deiminase (EC 3.5.4.21) from bacterium BN 11: purification, properties and applicability in a serum/urine creatinine assay (1991), Clin. Chim. Acta, 204, 223-238.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | analysis of creatinine in serum and urine | uncultured bacterium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
creatinine | - |
uncultured bacterium |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47500 | - |
6 * 47500, SDS-PAGE | uncultured bacterium |
| 285000 | - |
gel filtration, PAGE | uncultured bacterium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| creatinine + H2O | uncultured bacterium | - |
N-methylhydantoin + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured bacterium | - |
unclassified bacterium BN 11 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
uncultured bacterium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 78 | - |
- |
uncultured bacterium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50 mM potassium phosphate buffer, pH 7.65, 50% glycerol, loss in activity 0.038 U/ml per month | uncultured bacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| creatinine + H2O | - |
uncultured bacterium | N-methylhydantoin + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 47500, SDS-PAGE | uncultured bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 47 | - |
- |
uncultured bacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 8.75 | - |
uncultured bacterium |
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Release 2023.1 (January 2023)
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